ENSG00000132429

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254765 ENSG00000132429 HEK293_OSMI2_2hA HEK293_TMG_2hB POPDC3 protein_coding protein_coding 7.351895 9.329867 8.588101 0.263193 0.3420233 -0.119384 0.8849353 1.135919 0.7741742 0.03517677 0.08597304 -0.5472593 0.1226208 0.1217667 0.0898000 -0.03196667 3.516005e-01 1.283144e-06 FALSE TRUE
ENST00000474760 ENSG00000132429 HEK293_OSMI2_2hA HEK293_TMG_2hB POPDC3 protein_coding processed_transcript 7.351895 9.329867 8.588101 0.263193 0.3420233 -0.119384 3.7937986 3.609755 4.6350523 0.35750405 0.54007921 0.3598028 0.5270583 0.3893667 0.5424667 0.15310000 3.468206e-01 1.283144e-06   FALSE
ENST00000489134 ENSG00000132429 HEK293_OSMI2_2hA HEK293_TMG_2hB POPDC3 protein_coding processed_transcript 7.351895 9.329867 8.588101 0.263193 0.3420233 -0.119384 1.6821747 3.319803 1.4221576 0.08364678 0.15039998 -1.2172465 0.2248875 0.3559000 0.1655667 -0.19033333 1.283144e-06 1.283144e-06   FALSE
MSTRG.28701.4 ENSG00000132429 HEK293_OSMI2_2hA HEK293_TMG_2hB POPDC3 protein_coding   7.351895 9.329867 8.588101 0.263193 0.3420233 -0.119384 0.8642254 1.073714 1.5484558 0.53698553 0.78437804 0.5241330 0.1070500 0.1123000 0.1776667 0.06536667 9.475616e-01 1.283144e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132429 E001 13.501403 0.0014394683 0.0036743880 0.012223678 6 105157900 105158299 400 - 0.957 1.238 1.017
ENSG00000132429 E002 20.424123 0.0009640312 0.0591299008 0.125145034 6 105158300 105158359 60 - 1.223 1.369 0.509
ENSG00000132429 E003 62.810282 0.0005809566 0.0215002972 0.054643308 6 105158360 105158580 221 - 1.725 1.824 0.335
ENSG00000132429 E004 92.215977 0.0004488254 0.1981584472 0.323896045 6 105158581 105158751 171 - 1.925 1.969 0.145
ENSG00000132429 E005 70.100207 0.0004456023 0.9309619224 0.960550674 6 105159711 105159819 109 - 1.840 1.837 -0.012
ENSG00000132429 E006 1.764016 0.0078863519 0.8125842382 0.881131746 6 105159820 105160121 302 - 0.465 0.424 -0.211
ENSG00000132429 E007 10.468636 0.0017055308 0.2937496267 0.434799481 6 105161425 105161795 371 - 1.110 1.005 -0.383
ENSG00000132429 E008 101.568538 0.0004564239 0.0003670904 0.001646874 6 105161796 105162160 365 - 2.066 1.956 -0.368
ENSG00000132429 E009 1.063246 0.1263744394 0.6886992416 0.790774690 6 105163667 105163765 99 - 0.247 0.326 0.544
ENSG00000132429 E010 2.883702 0.4859195899 0.8680354392 0.919112614 6 105176193 105176261 69 - 0.566 0.581 0.066
ENSG00000132429 E011 27.449443 0.0009006728 0.0471742647 0.104141956 6 105179833 105180014 182 - 1.516 1.389 -0.439