ENSG00000132406

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254742 ENSG00000132406 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM128 protein_coding protein_coding 16.90753 11.799 23.32897 3.077241 0.3146671 0.9828544 1.920551 0.8871745 2.986905 0.07307442 0.3641668 1.740013 0.11176250 0.08353333 0.1279000 0.04436667 3.384947e-01 2.104958e-07 FALSE TRUE
ENST00000382753 ENSG00000132406 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM128 protein_coding protein_coding 16.90753 11.799 23.32897 3.077241 0.3146671 0.9828544 11.721438 9.6249176 14.721131 2.47962650 0.5836307 0.612524 0.72151667 0.81690000 0.6307000 -0.18620000 2.104958e-07 2.104958e-07 FALSE TRUE
MSTRG.24493.5 ENSG00000132406 HEK293_OSMI2_2hA HEK293_TMG_2hB TMEM128 protein_coding   16.90753 11.799 23.32897 3.077241 0.3146671 0.9828544 1.803303 0.7402899 2.682428 0.25265963 0.7357401 1.843388 0.09924583 0.06043333 0.1143667 0.05393333 2.991923e-01 2.104958e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132406 E001 85.46881 0.0017981746 0.011285379 0.031843155 4 4235542 4235829 288 - 1.895 1.998 0.348
ENSG00000132406 E002 91.04587 0.0056382613 0.425996306 0.568864876 4 4235830 4235969 140 - 1.950 1.995 0.154
ENSG00000132406 E003 200.52156 0.0003174990 0.827265722 0.891398495 4 4235970 4236250 281 - 2.309 2.314 0.017
ENSG00000132406 E004 62.34491 0.0004066491 0.823503085 0.888796704 4 4236251 4236254 4 - 1.806 1.816 0.033
ENSG00000132406 E005 57.72779 0.0004149923 0.420238795 0.563454606 4 4236255 4236256 2 - 1.760 1.797 0.123
ENSG00000132406 E006 14.59335 0.0354594531 0.198511777 0.324333658 4 4236836 4237077 242 - 1.265 1.105 -0.572
ENSG00000132406 E007 130.62179 0.0002475623 0.924591078 0.956521060 4 4237827 4237935 109 - 2.128 2.125 -0.010
ENSG00000132406 E008 145.53638 0.0003131957 0.862975185 0.915641095 4 4240321 4240479 159 - 2.176 2.172 -0.016
ENSG00000132406 E009 138.05516 0.0020500247 0.374145562 0.518566307 4 4246202 4246343 142 - 2.164 2.124 -0.135
ENSG00000132406 E010 34.45103 0.0113865083 0.001818135 0.006657415 4 4247601 4248105 505 - 1.647 1.376 -0.929
ENSG00000132406 E011 78.78448 0.0024079329 0.897254132 0.938386267 4 4248106 4248257 152 - 1.910 1.896 -0.047