ENSG00000132405

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000409757 ENSG00000132405 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D14 protein_coding protein_coding 24.33187 21.39206 25.91022 1.943589 0.7835375 0.2763282 5.112338 2.800442 5.749095 0.3579013 0.3661956 1.0350451 0.2063625 0.1304333 0.2217333 0.09130000 6.598689e-04 8.641202e-16 FALSE TRUE
ENST00000446947 ENSG00000132405 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D14 protein_coding protein_coding 24.33187 21.39206 25.91022 1.943589 0.7835375 0.2763282 4.866057 8.849748 0.000000 2.5469378 0.0000000 -9.7911218 0.2114292 0.4043000 0.0000000 -0.40430000 8.641202e-16 8.641202e-16 FALSE TRUE
ENST00000448507 ENSG00000132405 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D14 protein_coding protein_coding 24.33187 21.39206 25.91022 1.943589 0.7835375 0.2763282 3.235797 2.783169 4.302542 0.8358044 0.4863338 0.6266357 0.1336625 0.1367000 0.1664333 0.02973333 7.611368e-01 8.641202e-16 FALSE TRUE
ENST00000451522 ENSG00000132405 HEK293_OSMI2_2hA HEK293_TMG_2hB TBC1D14 protein_coding protein_coding 24.33187 21.39206 25.91022 1.943589 0.7835375 0.2763282 10.405538 6.464058 15.044206 0.8706187 0.4279543 1.2174245 0.4201792 0.3055333 0.5807000 0.27516667 1.632156e-04 8.641202e-16 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132405 E001 0.1472490 0.0429533699 5.690031e-01   4 6909242 6909443 202 + 0.106 0.000 -8.583
ENSG00000132405 E002 8.7546952 0.2001744775 5.483600e-01 6.782167e-01 4 6909444 6909505 62 + 0.991 0.975 -0.061
ENSG00000132405 E003 18.9995055 0.0062253991 2.853461e-03 9.833728e-03 4 6909826 6909951 126 + 1.372 1.184 -0.660
ENSG00000132405 E004 1.0955132 0.2150780782 5.953813e-01 7.174939e-01 4 6910248 6910601 354 + 0.261 0.401 0.881
ENSG00000132405 E005 157.0129902 0.0110829088 2.788234e-02 6.778168e-02 4 6923373 6924111 739 + 2.222 2.169 -0.174
ENSG00000132405 E006 0.1515154 0.0429525578 5.686543e-01   4 6954220 6954338 119 + 0.106 0.000 -10.657
ENSG00000132405 E007 58.3457198 0.0055230838 3.241375e-01 4.675058e-01 4 6967304 6967424 121 + 1.765 1.785 0.066
ENSG00000132405 E008 35.4842525 0.0006119475 2.483946e-04 1.168495e-03 4 6987162 6987349 188 + 1.623 1.486 -0.469
ENSG00000132405 E009 0.0000000       4 6987508 6987564 57 +      
ENSG00000132405 E010 83.4169781 0.0061012807 2.944435e-02 7.086337e-02 4 6994184 6994208 25 + 1.947 1.906 -0.135
ENSG00000132405 E011 148.2391133 0.0003004019 4.694848e-08 5.093488e-07 4 6994209 6994302 94 + 2.211 2.130 -0.270
ENSG00000132405 E012 149.5253156 0.0005713692 6.525622e-09 8.330589e-08 4 6996325 6996407 83 + 2.220 2.122 -0.327
ENSG00000132405 E013 168.3979640 0.0002453211 2.278832e-09 3.161610e-08 4 6999085 6999182 98 + 2.266 2.181 -0.283
ENSG00000132405 E014 109.3135957 0.0003165069 5.775416e-06 4.053253e-05 4 6999183 6999202 20 + 2.075 2.000 -0.251
ENSG00000132405 E015 176.6840410 0.0035653449 1.360232e-04 6.858796e-04 4 7001145 7001251 107 + 2.280 2.207 -0.244
ENSG00000132405 E016 0.8211642 0.1697747660 3.020270e-01 4.438540e-01 4 7001252 7001476 225 + 0.323 0.143 -1.502
ENSG00000132405 E017 141.3949346 0.0023753148 1.074420e-02 3.055414e-02 4 7004844 7004924 81 + 2.162 2.145 -0.057
ENSG00000132405 E018 142.6834103 0.0012721141 9.097091e-02 1.766737e-01 4 7006632 7006726 95 + 2.148 2.169 0.071
ENSG00000132405 E019 4.3241790 0.0037641094 5.652467e-01 6.923673e-01 4 7007524 7007625 102 + 0.733 0.719 -0.057
ENSG00000132405 E020 127.4161879 0.0003173410 8.439232e-01 9.028325e-01 4 7009877 7009948 72 + 2.069 2.155 0.290
ENSG00000132405 E021 167.3866576 0.0004978488 7.914253e-01 8.665954e-01 4 7010653 7010781 129 + 2.194 2.267 0.244
ENSG00000132405 E022 135.0949683 0.0003725899 4.080233e-01 5.516102e-01 4 7014448 7014557 110 + 2.109 2.163 0.183
ENSG00000132405 E023 234.5686260 0.0021795108 3.448304e-02 8.068161e-02 4 7025004 7025262 259 + 2.367 2.382 0.048
ENSG00000132405 E024 1847.2882800 0.0048890881 2.259141e-08 2.607931e-07 4 7030327 7033118 2792 + 3.169 3.357 0.623