ENSG00000132394

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254730 ENSG00000132394 HEK293_OSMI2_2hA HEK293_TMG_2hB EEFSEC protein_coding protein_coding 33.27218 56.78876 17.55433 2.075655 0.5514462 -1.693211 27.4056806 47.116500 13.821017 2.9798099 0.46642554 -1.768631 0.82040833 0.8285667 0.78736667 -0.04120000 6.284005e-01 1.832749e-21 FALSE  
MSTRG.23634.2 ENSG00000132394 HEK293_OSMI2_2hA HEK293_TMG_2hB EEFSEC protein_coding   33.27218 56.78876 17.55433 2.075655 0.5514462 -1.693211 0.2394867 0.088723 1.036820 0.0451826 0.09660321 3.406484 0.01153333 0.0016000 0.05886667 0.05726667 1.832749e-21 1.832749e-21 FALSE  
MSTRG.23634.4 ENSG00000132394 HEK293_OSMI2_2hA HEK293_TMG_2hB EEFSEC protein_coding   33.27218 56.78876 17.55433 2.075655 0.5514462 -1.693211 4.2994646 8.054036 1.481557 1.1996296 0.39773983 -2.434682 0.12075000 0.1427667 0.08450000 -0.05826667 3.966613e-01 1.832749e-21 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132394 E001 9.5901565 0.1037531431 1.584889e-01 2.731057e-01 3 128153481 128153494 14 + 1.087 0.868 -0.815
ENSG00000132394 E002 16.2879665 0.1961244547 1.869434e-01 3.098881e-01 3 128153495 128153500 6 + 1.308 1.079 -0.813
ENSG00000132394 E003 81.0263983 0.0156515861 7.655507e-02 1.539806e-01 3 128153501 128153605 105 + 1.894 1.778 -0.391
ENSG00000132394 E004 88.7377082 0.0134674773 4.859798e-02 1.066793e-01 3 128153606 128153650 45 + 1.937 1.815 -0.410
ENSG00000132394 E005 76.9791329 0.0170818193 5.366265e-02 1.156620e-01 3 128153651 128153667 17 + 1.883 1.753 -0.441
ENSG00000132394 E006 210.8084717 0.0085951742 5.260294e-05 2.947492e-04 3 128153668 128153823 156 + 2.361 2.172 -0.631
ENSG00000132394 E007 336.8278260 0.0002000472 5.949153e-09 7.663352e-08 3 128246836 128247043 208 + 2.511 2.389 -0.406
ENSG00000132394 E008 245.4465484 0.0001859111 9.157425e-01 9.507208e-01 3 128262128 128262224 97 + 2.268 2.280 0.038
ENSG00000132394 E009 331.5382085 0.0009060169 2.817619e-01 4.216765e-01 3 128264617 128264781 165 + 2.424 2.405 -0.065
ENSG00000132394 E010 1.2825016 0.2095854327 7.163120e-01 8.120103e-01 3 128336904 128337092 189 + 0.351 0.273 -0.512
ENSG00000132394 E011 1012.0837212 0.0001498465 4.307586e-01 5.733680e-01 3 128341233 128341889 657 + 2.877 2.896 0.063
ENSG00000132394 E012 470.5420589 0.0009910804 6.474491e-04 2.707009e-03 3 128358217 128358373 157 + 2.485 2.578 0.307
ENSG00000132394 E013 4.5412045 0.0459076346 3.777586e-01 5.220649e-01 3 128367678 128367794 117 + 0.777 0.638 -0.577
ENSG00000132394 E014 694.5900337 0.0044907028 1.267864e-07 1.262579e-06 3 128408069 128408646 578 + 2.577 2.760 0.610
ENSG00000132394 E015 0.9297165 0.0672480999 2.338569e-02 5.855061e-02 3 128434079 128434188 110 + 0.544 0.127 -2.882