ENSG00000132359

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000574709 ENSG00000132359 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1GAP2 protein_coding processed_transcript 4.111476 4.61794 4.208769 0.228243 0.1049964 -0.1335483 0.5502974 1.21685402 0.00000000 0.26320770 0.00000000 -6.9388198 0.12649583 0.26376667 0.00000000 -0.263766667 3.378375e-10 3.378375e-10 FALSE TRUE
ENST00000575979 ENSG00000132359 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1GAP2 protein_coding retained_intron 4.111476 4.61794 4.208769 0.228243 0.1049964 -0.1335483 0.2648119 0.06897494 0.07835138 0.06897494 0.07835138 0.1618577 0.06817917 0.01483333 0.01790000 0.003066667 1.000000e+00 3.378375e-10 FALSE FALSE
ENST00000637138 ENSG00000132359 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1GAP2 protein_coding protein_coding 4.111476 4.61794 4.208769 0.228243 0.1049964 -0.1335483 0.2307836 0.53536190 0.21913328 0.13822944 0.10957238 -1.2510269 0.05111667 0.11410000 0.05226667 -0.061833333 5.526516e-01 3.378375e-10 FALSE TRUE
MSTRG.13422.10 ENSG00000132359 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1GAP2 protein_coding   4.111476 4.61794 4.208769 0.228243 0.1049964 -0.1335483 1.6983713 1.75241479 2.20624185 0.24383683 0.12248545 0.3305618 0.41758333 0.37843333 0.52450000 0.146066667 1.726179e-01 3.378375e-10 FALSE TRUE
MSTRG.13422.5 ENSG00000132359 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1GAP2 protein_coding   4.111476 4.61794 4.208769 0.228243 0.1049964 -0.1335483 0.1086570 0.00000000 0.28738010 0.00000000 0.18385583 4.8942362 0.02528750 0.00000000 0.06733333 0.067333333 1.948927e-01 3.378375e-10 FALSE TRUE
MSTRG.13422.9 ENSG00000132359 HEK293_OSMI2_2hA HEK293_TMG_2hB RAP1GAP2 protein_coding   4.111476 4.61794 4.208769 0.228243 0.1049964 -0.1335483 0.6348063 0.32325007 1.16848794 0.08941556 0.09326412 1.8222599 0.15661250 0.07233333 0.27810000 0.205766667 1.026122e-03 3.378375e-10 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132359 E001 3.3483974 0.0051176390 3.072923e-06 2.293398e-05 17 2755705 2755767 63 + 0.000 0.835 13.382
ENSG00000132359 E002 6.5078210 0.0032560549 5.027229e-04 2.170153e-03 17 2770329 2770445 117 + 0.517 1.017 2.039
ENSG00000132359 E003 0.2214452 0.0388328059 5.285619e-01   17 2776613 2776720 108 + 0.000 0.122 11.760
ENSG00000132359 E004 0.6589510 0.0190084335 3.598368e-01 5.041488e-01 17 2776887 2776947 61 + 0.123 0.295 1.571
ENSG00000132359 E005 0.2214452 0.0388328059 5.285619e-01   17 2777056 2777278 223 + 0.000 0.122 11.760
ENSG00000132359 E006 0.1515154 0.0428343392 4.479521e-01   17 2786466 2786499 34 + 0.123 0.000 -12.388
ENSG00000132359 E007 0.0000000       17 2796438 2796481 44 +      
ENSG00000132359 E008 0.0000000       17 2796482 2796571 90 +      
ENSG00000132359 E009 1.8788966 0.0073934741 1.720505e-01 2.908686e-01 17 2800515 2800550 36 + 0.297 0.555 1.401
ENSG00000132359 E010 4.8168895 0.0040127253 5.061848e-01 6.418558e-01 17 2905284 2905368 85 + 0.692 0.813 0.489
ENSG00000132359 E011 4.9350068 0.0397854051 7.236324e-01 8.174150e-01 17 2957759 2957794 36 + 0.720 0.813 0.371
ENSG00000132359 E012 2.2099409 0.0227722242 7.425609e-01 8.314279e-01 17 2962670 2962714 45 + 0.472 0.554 0.396
ENSG00000132359 E013 4.8391163 0.0034991744 3.289417e-01 4.724193e-01 17 2963430 2963462 33 + 0.817 0.717 -0.401
ENSG00000132359 E014 12.9702480 0.0036321596 8.151859e-02 1.619237e-01 17 2963856 2964068 213 + 1.007 1.211 0.737
ENSG00000132359 E015 10.1593682 0.0019536057 4.924219e-03 1.571905e-02 17 2965540 2965643 104 + 0.817 1.155 1.258
ENSG00000132359 E016 0.2998086 0.0292434321 9.442772e-01   17 2975611 2975614 4 + 0.123 0.122 -0.016
ENSG00000132359 E017 11.4099990 0.0066742147 3.093468e-02 7.378452e-02 17 2975615 2975771 157 + 1.187 1.003 -0.668
ENSG00000132359 E018 3.2202744 0.0049617672 2.938693e-02 7.074754e-02 17 2975772 2975775 4 + 0.771 0.469 -1.337
ENSG00000132359 E019 22.9041748 0.0009495471 2.905584e-01 4.313803e-01 17 2980287 2980365 79 + 1.396 1.356 -0.137
ENSG00000132359 E020 20.2980323 0.0635908560 3.089792e-01 4.512655e-01 17 2981195 2981248 54 + 1.367 1.287 -0.278
ENSG00000132359 E021 27.6375201 0.0008378267 2.219248e-01 3.526948e-01 17 2984983 2985066 84 + 1.479 1.435 -0.149
ENSG00000132359 E022 23.9229230 0.0009839408 2.549732e-01 3.917465e-01 17 2991297 2991344 48 + 1.418 1.374 -0.151
ENSG00000132359 E023 23.8620570 0.0007978811 2.191599e-02 5.550174e-02 17 2991345 2991397 53 + 1.454 1.331 -0.428
ENSG00000132359 E024 38.5008090 0.0006641003 4.735692e-04 2.059082e-03 17 2995337 2995466 130 + 1.676 1.519 -0.534
ENSG00000132359 E025 43.1863804 0.0043685422 3.010737e-03 1.029781e-02 17 2998221 2998376 156 + 1.716 1.575 -0.479
ENSG00000132359 E026 0.3697384 0.0250531687 2.252665e-01 3.567403e-01 17 3005341 3005368 28 + 0.000 0.217 12.808
ENSG00000132359 E027 24.0393406 0.0008338998 5.671641e-01 6.940272e-01 17 3005369 3005440 72 + 1.401 1.397 -0.014
ENSG00000132359 E028 29.8912297 0.0007488880 2.457436e-02 6.100047e-02 17 3005955 3006041 87 + 1.540 1.435 -0.360
ENSG00000132359 E029 29.0190955 0.0008108989 8.217607e-02 1.629485e-01 17 3008011 3008100 90 + 1.510 1.435 -0.258
ENSG00000132359 E030 18.9609364 0.0010155381 6.018928e-02 1.269348e-01 17 3008101 3008145 45 + 1.354 1.244 -0.385
ENSG00000132359 E031 29.8491379 0.0006988815 3.509789e-02 8.183031e-02 17 3018061 3018198 138 + 1.536 1.440 -0.328
ENSG00000132359 E032 28.3077777 0.0009658363 1.531784e-03 5.739327e-03 17 3020477 3020595 119 + 1.544 1.375 -0.584
ENSG00000132359 E033 30.2810139 0.0007029002 4.258600e-02 9.579423e-02 17 3026008 3026121 114 + 1.536 1.446 -0.310
ENSG00000132359 E034 0.3289534 0.0289725897 9.451981e-01   17 3026122 3026248 127 + 0.123 0.122 -0.013
ENSG00000132359 E035 22.5705737 0.0008565472 6.437676e-01 7.562234e-01 17 3026350 3026464 115 + 1.366 1.368 0.008
ENSG00000132359 E036 27.7218394 0.0013001858 8.127531e-01 8.812795e-01 17 3026944 3027070 127 + 1.423 1.475 0.178
ENSG00000132359 E037 21.5348156 0.0010127730 4.241987e-01 5.671754e-01 17 3030922 3030998 77 + 1.366 1.343 -0.079
ENSG00000132359 E038 16.0068076 0.0309131082 8.478598e-01 9.055287e-01 17 3032411 3032449 39 + 1.197 1.256 0.211
ENSG00000132359 E039 0.5212538 0.0203582629 6.225554e-01 7.393277e-01 17 3033081 3033391 311 + 0.123 0.217 0.987
ENSG00000132359 E040 72.1366183 0.0004677363 1.396524e-01 2.475438e-01 17 3033392 3034212 821 + 1.873 1.849 -0.079
ENSG00000132359 E041 11.4966988 0.0227984802 4.124433e-01 5.559866e-01 17 3034213 3034229 17 + 1.117 1.060 -0.208
ENSG00000132359 E042 24.5618252 0.0110525257 2.859605e-01 4.263281e-01 17 3034230 3034400 171 + 1.423 1.373 -0.174
ENSG00000132359 E043 33.4411108 0.0057847528 1.322893e-01 2.372941e-01 17 3034401 3034693 293 + 1.444 1.585 0.484
ENSG00000132359 E044 16.7600662 0.0011427874 5.497870e-03 1.728120e-02 17 3034694 3034751 58 + 1.072 1.344 0.965
ENSG00000132359 E045 267.1091396 0.0062936159 7.516560e-08 7.818965e-07 17 3034752 3037741 2990 + 2.285 2.513 0.760