ENSG00000132323

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254654 ENSG00000132323 HEK293_OSMI2_2hA HEK293_TMG_2hB ILKAP protein_coding protein_coding 58.79784 71.86457 54.61268 7.150399 0.2227718 -0.3959812 44.901080 60.084082 37.767321 6.9214032 0.4302403 -0.6697025 0.7539750 0.83340000 0.6915667 -0.1418333 6.835556e-06 2.395144e-06 FALSE TRUE
ENST00000479400 ENSG00000132323 HEK293_OSMI2_2hA HEK293_TMG_2hB ILKAP protein_coding retained_intron 58.79784 71.86457 54.61268 7.150399 0.2227718 -0.3959812 4.673954 2.781852 6.056909 0.1519758 0.3117831 1.1197393 0.0842125 0.03986667 0.1109667 0.0711000 2.395144e-06 2.395144e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132323 E001 0.2965864 0.0610299485 4.015995e-01   2 238170402 238170402 1 - 0.000 0.175 10.919
ENSG00000132323 E002 0.2965864 0.0610299485 4.015995e-01   2 238170403 238170403 1 - 0.000 0.175 10.921
ENSG00000132323 E003 1.0380523 0.8380436236 3.761870e-01 5.205401e-01 2 238170404 238170407 4 - 0.000 0.407 12.570
ENSG00000132323 E004 1.1863455 0.9238658305 3.878549e-01 5.319918e-01 2 238170408 238170408 1 - 0.000 0.441 12.749
ENSG00000132323 E005 383.4944486 0.0013169893 2.726011e-08 3.094090e-07 2 238170409 238170676 268 - 2.463 2.605 0.473
ENSG00000132323 E006 21.5551534 0.0215766050 1.204242e-01 2.205743e-01 2 238170677 238170942 266 - 1.211 1.369 0.551
ENSG00000132323 E007 347.7222937 0.0001738271 1.062216e-13 3.006629e-12 2 238170943 238171024 82 - 2.413 2.569 0.519
ENSG00000132323 E008 492.7446787 0.0027929287 6.813621e-03 2.073840e-02 2 238173534 238173653 120 - 2.616 2.694 0.262
ENSG00000132323 E009 10.7552090 0.0040038046 1.492710e-02 4.032418e-02 2 238173654 238173926 273 - 1.200 0.953 -0.897
ENSG00000132323 E010 657.4295064 0.0014604921 5.900939e-02 1.249389e-01 2 238182065 238182186 122 - 2.768 2.808 0.135
ENSG00000132323 E011 513.6885967 0.0001712040 9.679607e-02 1.855801e-01 2 238183653 238183715 63 - 2.673 2.701 0.094
ENSG00000132323 E012 324.4463167 0.0001636220 3.348712e-01 4.786086e-01 2 238183716 238183722 7 - 2.477 2.497 0.067
ENSG00000132323 E013 345.5026603 0.0001767980 9.949888e-02 1.897137e-01 2 238183723 238183740 18 - 2.495 2.528 0.111
ENSG00000132323 E014 519.0518957 0.0001594136 1.395612e-03 5.292441e-03 2 238184020 238184113 94 - 2.658 2.711 0.175
ENSG00000132323 E015 481.3617907 0.0003259583 2.912778e-01 4.321368e-01 2 238185181 238185287 107 - 2.674 2.653 -0.068
ENSG00000132323 E016 18.4193310 0.0501296080 3.054286e-04 1.401505e-03 2 238185288 238187057 1770 - 1.523 1.039 -1.701
ENSG00000132323 E017 487.7663420 0.0001578556 4.137244e-03 1.352750e-02 2 238188131 238188257 127 - 2.700 2.652 -0.159
ENSG00000132323 E018 45.8080459 0.0008585552 5.842805e-23 5.160382e-21 2 238188258 238188594 337 - 1.918 1.397 -1.773
ENSG00000132323 E019 8.6548433 0.0019632287 2.673152e-05 1.609422e-04 2 238189691 238189697 7 - 1.212 0.759 -1.688
ENSG00000132323 E020 17.4427411 0.0010349008 8.102188e-11 1.452162e-09 2 238189698 238189852 155 - 1.522 0.999 -1.844
ENSG00000132323 E021 198.7642957 0.0001854778 1.291501e-01 2.329930e-01 2 238189853 238189858 6 - 2.306 2.267 -0.130
ENSG00000132323 E022 197.4857119 0.0001963568 3.661240e-01 5.104597e-01 2 238189859 238189862 4 - 2.293 2.269 -0.077
ENSG00000132323 E023 399.5866211 0.0006274775 1.617650e-03 6.016905e-03 2 238189863 238189972 110 - 2.625 2.555 -0.231
ENSG00000132323 E024 279.2026951 0.0020931647 1.260574e-04 6.415539e-04 2 238194275 238194331 57 - 2.501 2.379 -0.408
ENSG00000132323 E025 4.6044089 0.0549899378 6.125411e-03 1.893762e-02 2 238194332 238194408 77 - 0.978 0.512 -1.920
ENSG00000132323 E026 8.5238182 0.0213099832 2.787195e-07 2.595742e-06 2 238194409 238194702 294 - 1.284 0.626 -2.499
ENSG00000132323 E027 3.8543125 0.0110993627 1.087291e-08 1.331334e-07 2 238194703 238194781 79 - 1.049 0.176 -4.352
ENSG00000132323 E028 13.8400876 0.0012708048 8.792356e-05 4.652750e-04 2 238194782 238194804 23 - 1.355 1.010 -1.229
ENSG00000132323 E029 272.0363969 0.0056854331 2.857832e-02 6.916650e-02 2 238194805 238194870 66 - 2.477 2.373 -0.346
ENSG00000132323 E030 216.9590991 0.0094139776 3.175038e-02 7.536856e-02 2 238203499 238203715 217 - 2.393 2.266 -0.424