ENSG00000132300

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254630 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding protein_coding 44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 8.498169 3.8587732 13.065829 0.32137198 0.2412422 1.7569544 0.21517917 0.26450000 0.19186667 -0.072633333 5.607272e-03 2.140639e-17 FALSE TRUE
ENST00000409783 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding protein_coding 44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 1.452487 0.0000000 3.473514 0.00000000 0.1491710 8.4443995 0.02223333 0.00000000 0.05093333 0.050933333 2.140639e-17 2.140639e-17 FALSE FALSE
ENST00000464541 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding retained_intron 44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 2.305998 0.7364000 3.110299 0.06166436 0.2119345 2.0636631 0.05113333 0.05050000 0.04560000 -0.004900000 8.420809e-01 2.140639e-17   FALSE
ENST00000467273 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding retained_intron 44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 2.513154 0.5576658 2.694716 0.22313500 0.2773814 2.2523633 0.05161667 0.03690000 0.03946667 0.002566667 9.208659e-01 2.140639e-17 FALSE FALSE
ENST00000476215 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding retained_intron 44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 2.624434 0.5122298 4.317142 0.14605321 0.2301179 3.0506579 0.05572917 0.03453333 0.06346667 0.028933333 1.366150e-01 2.140639e-17   FALSE
ENST00000484203 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding retained_intron 44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 7.614652 1.6509479 11.825108 0.18328519 0.2204495 2.8329942 0.14752917 0.11503333 0.17363333 0.058600000 8.902132e-02 2.140639e-17 FALSE TRUE
ENST00000487043 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding retained_intron 44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 3.229550 2.7190116 4.074109 0.59595856 0.4825427 0.5816427 0.10721667 0.18530000 0.06003333 -0.125266667 1.344480e-03 2.140639e-17 FALSE TRUE
MSTRG.18785.6 ENSG00000132300 HEK293_OSMI2_2hA HEK293_TMG_2hB PTCD3 protein_coding   44.19304 14.54548 68.15875 0.8015377 1.080757 2.227548 9.610847 3.2458055 14.475989 0.63053593 1.1062826 2.1535718 0.20375000 0.22753333 0.21230000 -0.015233333 9.857031e-01 2.140639e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132300 E001 1.6396603 0.3242475052 4.383122e-01 5.802064e-01 2 86106223 86106223 1 + 0.441 0.204 -1.553
ENSG00000132300 E002 1.6396603 0.3242475052 4.383122e-01 5.802064e-01 2 86106224 86106225 2 + 0.441 0.204 -1.553
ENSG00000132300 E003 1.9384248 0.0946660502 1.919479e-01 3.162144e-01 2 86106226 86106233 8 + 0.494 0.204 -1.826
ENSG00000132300 E004 2.0835974 0.1315739844 1.863819e-01 3.092089e-01 2 86106234 86106234 1 + 0.518 0.204 -1.939
ENSG00000132300 E005 2.2287701 0.1679850950 1.846927e-01 3.070895e-01 2 86106235 86106235 1 + 0.540 0.204 -2.044
ENSG00000132300 E006 15.7962717 0.0011156622 2.209096e-02 5.586569e-02 2 86106236 86106243 8 + 1.225 0.998 -0.816
ENSG00000132300 E007 17.2753296 0.0010697571 4.894701e-02 1.072838e-01 2 86106244 86106247 4 + 1.251 1.070 -0.647
ENSG00000132300 E008 79.0249621 0.0003387776 1.158678e-01 2.140463e-01 2 86106248 86106351 104 + 1.862 1.804 -0.194
ENSG00000132300 E009 0.0000000       2 86107118 86107200 83 +      
ENSG00000132300 E010 0.3030308 0.3805673506 1.000000e+00   2 86108345 86108349 5 + 0.132 0.000 -9.895
ENSG00000132300 E011 92.0768961 0.0004047779 2.137619e-02 5.438406e-02 2 86108350 86108402 53 + 1.933 1.847 -0.289
ENSG00000132300 E012 81.4689595 0.0003179437 2.036556e-03 7.340134e-03 2 86108500 86108536 37 + 1.892 1.762 -0.439
ENSG00000132300 E013 0.7727888 0.0665127501 1.002852e-01 1.909153e-01 2 86108537 86108925 389 + 0.132 0.444 2.329
ENSG00000132300 E014 0.4513240 0.1809263373 7.018881e-01 8.008506e-01 2 86110978 86111112 135 + 0.132 0.202 0.735
ENSG00000132300 E015 101.9582555 0.0002994029 1.701316e-02 4.496314e-02 2 86111113 86111158 46 + 1.979 1.896 -0.280
ENSG00000132300 E016 0.7363589 0.0155462002 2.163284e-01 3.459833e-01 2 86111790 86112095 306 + 0.276 0.000 -11.209
ENSG00000132300 E017 128.9814850 0.0026047708 1.557371e-02 4.178670e-02 2 86116530 86116598 69 + 2.082 1.990 -0.310
ENSG00000132300 E018 171.8582120 0.0007755653 2.351196e-03 8.311446e-03 2 86117055 86117159 105 + 2.204 2.114 -0.299
ENSG00000132300 E019 183.4228108 0.0042733636 6.856340e-03 2.085236e-02 2 86118921 86119044 124 + 2.236 2.128 -0.360
ENSG00000132300 E020 0.8232405 0.1411811690 9.052306e-01 9.436745e-01 2 86119045 86119181 137 + 0.233 0.205 -0.237
ENSG00000132300 E021 7.2599274 0.0396851422 4.256224e-01 5.685419e-01 2 86119544 86119614 71 + 0.889 0.766 -0.479
ENSG00000132300 E022 0.6611159 0.0290115179 9.643258e-01 9.815162e-01 2 86121465 86121478 14 + 0.185 0.204 0.171
ENSG00000132300 E023 103.7949630 0.0002571353 8.292792e-04 3.363689e-03 2 86121479 86121493 15 + 1.996 1.872 -0.416
ENSG00000132300 E024 182.5744595 0.0002854339 1.524123e-06 1.216877e-05 2 86121494 86121594 101 + 2.241 2.102 -0.465
ENSG00000132300 E025 161.5016687 0.0001994604 1.013351e-04 5.280166e-04 2 86123701 86123762 62 + 2.184 2.070 -0.381
ENSG00000132300 E026 183.5322365 0.0017073884 1.535371e-02 4.129262e-02 2 86124995 86125082 88 + 2.229 2.155 -0.249
ENSG00000132300 E027 162.8674615 0.0005061135 1.466611e-01 2.571473e-01 2 86125455 86125515 61 + 2.167 2.134 -0.111
ENSG00000132300 E028 142.3319029 0.0045001565 4.575696e-01 5.978949e-01 2 86125795 86125880 86 + 2.107 2.084 -0.076
ENSG00000132300 E029 185.9268198 0.0059450649 2.976813e-01 4.390274e-01 2 86127161 86127305 145 + 2.226 2.188 -0.127
ENSG00000132300 E030 43.2552678 0.0008353499 5.265943e-04 2.259199e-03 2 86127306 86127661 356 + 1.642 1.426 -0.740
ENSG00000132300 E031 16.7301577 0.0116814236 8.783750e-01 9.258947e-01 2 86127719 86127871 153 + 1.187 1.219 0.114
ENSG00000132300 E032 12.4267919 0.0094454333 3.327003e-01 4.763778e-01 2 86127872 86127929 58 + 1.098 0.999 -0.360
ENSG00000132300 E033 16.4909308 0.0011960475 8.250319e-01 8.897708e-01 2 86127930 86127940 11 + 1.191 1.186 -0.020
ENSG00000132300 E034 106.2311794 0.0003090753 2.532329e-01 3.897301e-01 2 86127941 86127948 8 + 1.982 1.952 -0.101
ENSG00000132300 E035 127.9664634 0.0003370256 7.714070e-01 8.524407e-01 2 86127949 86127991 43 + 2.048 2.074 0.087
ENSG00000132300 E036 99.5532167 0.0004467600 6.443335e-01 7.566577e-01 2 86130648 86130650 3 + 1.949 1.946 -0.011
ENSG00000132300 E037 172.3550957 0.0012336468 5.565311e-01 6.851558e-01 2 86130651 86130737 87 + 2.174 2.209 0.118
ENSG00000132300 E038 15.6089355 0.0011356431 5.137568e-01 6.485274e-01 2 86130738 86130889 152 + 1.181 1.132 -0.177
ENSG00000132300 E039 21.4472447 0.0011767956 8.783763e-02 1.718415e-01 2 86130890 86131077 188 + 1.337 1.202 -0.475
ENSG00000132300 E040 130.6805675 0.0027328906 3.242835e-02 7.668417e-02 2 86131078 86131106 29 + 2.034 2.139 0.353
ENSG00000132300 E041 221.7316552 0.0003140630 3.030693e-01 4.449750e-01 2 86132318 86132424 107 + 2.282 2.326 0.148
ENSG00000132300 E042 26.8909176 0.0013323675 4.698229e-04 2.044670e-03 2 86132425 86132823 399 + 1.454 1.168 -1.000
ENSG00000132300 E043 10.0119034 0.0016545475 2.342663e-02 5.863682e-02 2 86132824 86132877 54 + 1.045 0.762 -1.079
ENSG00000132300 E044 14.4410909 0.0044072181 2.166835e-01 3.463963e-01 2 86132878 86133063 186 + 1.166 1.047 -0.427
ENSG00000132300 E045 8.8480505 0.0130231486 2.273845e-01 3.592756e-01 2 86133064 86133177 114 + 0.898 1.069 0.631
ENSG00000132300 E046 179.2605665 0.0002442368 2.353068e-03 8.317600e-03 2 86133178 86133256 79 + 2.172 2.278 0.354
ENSG00000132300 E047 7.0450738 0.0023837802 9.559536e-01 9.763836e-01 2 86133257 86133268 12 + 0.858 0.879 0.083
ENSG00000132300 E048 10.3963440 0.0015743420 6.381055e-02 1.330399e-01 2 86133269 86133322 54 + 1.059 0.843 -0.808
ENSG00000132300 E049 9.2965619 0.0017644395 9.123670e-02 1.770610e-01 2 86133323 86133345 23 + 1.009 0.805 -0.778
ENSG00000132300 E050 204.4797150 0.0007437127 1.962790e-03 7.109568e-03 2 86133346 86133436 91 + 2.228 2.337 0.363
ENSG00000132300 E051 86.2019269 0.0002921204 7.137310e-02 1.456139e-01 2 86134292 86134295 4 + 1.862 1.954 0.310
ENSG00000132300 E052 190.0463484 0.0002817150 1.473897e-02 3.990132e-02 2 86134296 86134377 82 + 2.205 2.291 0.290
ENSG00000132300 E053 245.6539318 0.0011212119 1.108059e-03 4.329913e-03 2 86134839 86134987 149 + 2.308 2.418 0.370
ENSG00000132300 E054 9.5448506 0.0090552458 1.693453e-02 4.478719e-02 2 86134988 86135198 211 + 1.039 0.714 -1.247
ENSG00000132300 E055 140.7324622 0.0030591465 4.920838e-04 2.130147e-03 2 86136521 86136562 42 + 2.050 2.212 0.541
ENSG00000132300 E056 22.4637976 0.0269637127 1.261834e-02 3.501036e-02 2 86136563 86136881 319 + 1.229 1.515 0.996
ENSG00000132300 E057 277.2314839 0.0029867118 1.828386e-03 6.691048e-03 2 86136982 86137140 159 + 2.355 2.480 0.416
ENSG00000132300 E058 825.1590503 0.0052103496 4.209983e-02 9.490890e-02 2 86137469 86142158 4690 + 2.839 2.925 0.289