ENSG00000132294

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254624 ENSG00000132294 HEK293_OSMI2_2hA HEK293_TMG_2hB EFR3A protein_coding protein_coding 6.692945 2.275936 10.50308 0.1770537 0.2915359 2.201328 4.46084654 0.4133309 10.02610055 0.2998890 0.27519176 4.567269 0.5766958 0.1992667 0.955066667 0.7558000 0.001386238 0.001386238 FALSE TRUE
ENST00000519656 ENSG00000132294 HEK293_OSMI2_2hA HEK293_TMG_2hB EFR3A protein_coding protein_coding 6.692945 2.275936 10.50308 0.1770537 0.2915359 2.201328 0.96775271 0.6155266 0.00000000 0.3734243 0.00000000 -5.966999 0.1411833 0.2619333 0.000000000 -0.2619333 0.055798887 0.001386238 FALSE TRUE
ENST00000521940 ENSG00000132294 HEK293_OSMI2_2hA HEK293_TMG_2hB EFR3A protein_coding processed_transcript 6.692945 2.275936 10.50308 0.1770537 0.2915359 2.201328 0.40107689 0.9238281 0.11000422 0.5175241 0.05807243 -2.960072 0.1730125 0.3957333 0.010600000 -0.3851333 0.221785047 0.001386238   FALSE
ENST00000523074 ENSG00000132294 HEK293_OSMI2_2hA HEK293_TMG_2hB EFR3A protein_coding retained_intron 6.692945 2.275936 10.50308 0.1770537 0.2915359 2.201328 0.08066237 0.3232505 0.08877393 0.3232505 0.02652497 -1.754405 0.0213875 0.1430667 0.008333333 -0.1347333 0.962493498 0.001386238   FALSE
MSTRG.32110.3 ENSG00000132294 HEK293_OSMI2_2hA HEK293_TMG_2hB EFR3A protein_coding   6.692945 2.275936 10.50308 0.1770537 0.2915359 2.201328 0.52620784 0.0000000 0.00000000 0.0000000 0.00000000 0.000000 0.0621750 0.0000000 0.000000000 0.0000000   0.001386238 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132294 E001 2.8225252 0.0057611303 2.545739e-01 3.912886e-01 8 131904093 131904108 16 + 0.458 0.305 -0.880
ENSG00000132294 E002 22.9114390 0.0009576517 3.106741e-04 1.422955e-03 8 131904109 131904322 214 + 1.212 0.910 -1.101
ENSG00000132294 E003 0.3686942 0.0280545108 1.735158e-01 2.927619e-01 8 131905278 131905330 53 + 0.043 0.305 3.294
ENSG00000132294 E004 0.0000000       8 131938225 131938315 91 +      
ENSG00000132294 E005 0.0000000       8 131939881 131939900 20 +      
ENSG00000132294 E006 0.0000000       8 131939901 131939944 44 +      
ENSG00000132294 E007 0.0000000       8 131939945 131940219 275 +      
ENSG00000132294 E008 26.2233891 0.0036210643 4.001119e-03 1.314534e-02 8 131940499 131940575 77 + 1.257 1.087 -0.606
ENSG00000132294 E009 0.1515154 0.0424598593 8.411361e-01   8 131940576 131940644 69 + 0.043 0.000 -6.873
ENSG00000132294 E010 42.0860392 0.0005405181 9.180924e-05 4.834608e-04 8 131944745 131944872 128 + 1.453 1.263 -0.660
ENSG00000132294 E011 49.8007350 0.0016105139 4.943268e-07 4.375700e-06 8 131946483 131946633 151 + 1.530 1.238 -1.012
ENSG00000132294 E012 44.1259602 0.0005419395 3.242598e-06 2.408077e-05 8 131949969 131950090 122 + 1.479 1.211 -0.931
ENSG00000132294 E013 52.3128518 0.0020488164 2.071145e-05 1.280254e-04 8 131953818 131953967 150 + 1.544 1.350 -0.669
ENSG00000132294 E014 43.7953001 0.0005231184 1.017032e-03 4.017864e-03 8 131955768 131955905 138 + 1.464 1.350 -0.393
ENSG00000132294 E015 28.5123381 0.0007168161 4.699096e-03 1.509324e-02 8 131959585 131959663 79 + 1.288 1.153 -0.476
ENSG00000132294 E016 45.8843124 0.0004789449 2.477816e-05 1.504486e-04 8 131968295 131968430 136 + 1.491 1.286 -0.709
ENSG00000132294 E017 58.5469163 0.0010086668 3.844488e-07 3.477480e-06 8 131970476 131970643 168 + 1.595 1.350 -0.843
ENSG00000132294 E018 55.5362317 0.0004149175 1.752760e-06 1.380744e-05 8 131976027 131976141 115 + 1.570 1.350 -0.759
ENSG00000132294 E019 43.4833351 0.0005294740 2.729218e-04 1.269469e-03 8 131977041 131977092 52 + 1.462 1.309 -0.532
ENSG00000132294 E020 66.7206562 0.0067341950 2.130777e-02 5.423768e-02 8 131978847 131979019 173 + 1.630 1.622 -0.027
ENSG00000132294 E021 45.8107011 0.0111282126 4.345681e-01 5.769039e-01 8 131979346 131979421 76 + 1.458 1.554 0.329
ENSG00000132294 E022 52.3988419 0.0004450649 2.987517e-02 7.171988e-02 8 131984139 131984300 162 + 1.528 1.539 0.036
ENSG00000132294 E023 42.2307467 0.0005746308 8.280139e-01 8.919081e-01 8 131984929 131985060 132 + 1.414 1.599 0.632
ENSG00000132294 E024 34.5703795 0.0007176170 8.680899e-02 1.702939e-01 8 131986194 131986261 68 + 1.355 1.369 0.050
ENSG00000132294 E025 42.2609377 0.0005205090 2.952933e-01 4.364330e-01 8 131987575 131987702 128 + 1.426 1.513 0.297
ENSG00000132294 E026 0.4418608 0.0245763347 1.000000e+00 1.000000e+00 8 131996390 131996405 16 + 0.118 0.000 -8.458
ENSG00000132294 E027 41.2646107 0.0005678422 1.897474e-01 3.134446e-01 8 131996406 131996497 92 + 1.421 1.485 0.218
ENSG00000132294 E028 1.6263095 0.0082896560 1.589503e-01 2.737316e-01 8 132000755 132000768 14 + 0.331 0.000 -10.332
ENSG00000132294 E029 3.4460758 0.0050703264 9.541827e-01 9.752846e-01 8 132000769 132000998 230 + 0.474 0.608 0.629
ENSG00000132294 E030 42.2275015 0.0005019682 1.542238e-01 2.674126e-01 8 132001759 132001807 49 + 1.430 1.485 0.189
ENSG00000132294 E031 60.3993359 0.0004575003 6.282061e-01 7.437910e-01 8 132002603 132002706 104 + 1.568 1.707 0.472
ENSG00000132294 E032 43.4643419 0.0006402938 1.848529e-01 3.073015e-01 8 132003236 132003285 50 + 1.409 1.671 0.895
ENSG00000132294 E033 391.0367250 0.0177663748 1.580899e-14 5.066766e-13 8 132010790 132013642 2853 + 2.291 2.816 1.748