ENSG00000132254

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000396777 ENSG00000132254 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFIP2 protein_coding protein_coding 130.9985 220.5917 90.69837 5.478793 2.377012 -1.282137 75.981707 132.30535 48.192269 1.559088 1.5244045 -1.456808 0.57156667 0.60020000 0.53116667 -0.06903333 0.003391121 6.073989e-29 FALSE  
ENST00000423813 ENSG00000132254 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFIP2 protein_coding protein_coding 130.9985 220.5917 90.69837 5.478793 2.377012 -1.282137 8.891259 18.55341 6.204393 2.729424 0.4654466 -1.578776 0.06442917 0.08366667 0.06826667 -0.01540000 0.622089502 6.073989e-29 FALSE  
ENST00000531037 ENSG00000132254 HEK293_OSMI2_2hA HEK293_TMG_2hB ARFIP2 protein_coding nonsense_mediated_decay 130.9985 220.5917 90.69837 5.478793 2.377012 -1.282137 26.304251 54.30054 14.748816 1.973379 1.3871500 -1.879655 0.18327083 0.24613333 0.16283333 -0.08330000 0.007903595 6.073989e-29 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000132254 E001 133.27508 1.192590e-03 3.104352e-16 1.256646e-14 11 6474683 6475679 997 - 2.247 1.982 -0.885
ENSG00000132254 E002 92.53238 1.903753e-03 3.048525e-29 4.796794e-27 11 6475680 6476518 839 - 2.195 1.749 -1.499
ENSG00000132254 E003 38.81029 5.852117e-04 2.798286e-01 4.195980e-01 11 6476519 6476553 35 - 1.564 1.522 -0.143
ENSG00000132254 E004 904.90616 1.978879e-03 1.016020e-04 5.292620e-04 11 6476554 6476733 180 - 2.787 2.914 0.424
ENSG00000132254 E005 1291.98835 2.946629e-03 1.113141e-04 5.737797e-04 11 6476734 6476813 80 - 2.933 3.070 0.456
ENSG00000132254 E006 3103.32011 1.399948e-03 1.139420e-12 2.768141e-11 11 6476814 6477268 455 - 3.302 3.453 0.501
ENSG00000132254 E007 1800.39075 6.558886e-05 3.693981e-04 1.655736e-03 11 6477718 6477892 175 - 3.137 3.199 0.208
ENSG00000132254 E008 1057.00013 8.886634e-05 2.746769e-01 4.138668e-01 11 6478041 6478083 43 - 2.944 2.957 0.043
ENSG00000132254 E009 1451.52100 2.689230e-04 4.388333e-01 5.806683e-01 11 6478084 6478198 115 - 3.078 3.095 0.056
ENSG00000132254 E010 20.35509 1.764452e-02 2.408196e-05 1.466633e-04 11 6478579 6478652 74 - 1.517 1.129 -1.359
ENSG00000132254 E011 1162.43252 7.010420e-04 1.055354e-01 1.989172e-01 11 6478738 6478858 121 - 2.953 3.007 0.179
ENSG00000132254 E012 1166.92579 1.374552e-03 6.870896e-01 7.895011e-01 11 6478859 6478959 101 - 2.982 3.000 0.060
ENSG00000132254 E013 1345.94398 1.195791e-03 3.275880e-03 1.107987e-02 11 6479140 6479258 119 - 3.080 3.051 -0.098
ENSG00000132254 E014 35.63310 6.171117e-04 8.973011e-20 5.546199e-18 11 6479259 6479305 47 - 1.819 1.308 -1.747
ENSG00000132254 E015 35.31804 1.546278e-03 8.346556e-23 7.256677e-21 11 6479306 6479357 52 - 1.846 1.277 -1.947
ENSG00000132254 E016 64.27672 3.876049e-04 1.020340e-37 3.048902e-35 11 6479358 6479656 299 - 2.087 1.545 -1.831
ENSG00000132254 E017 60.39892 4.448192e-04 3.447749e-33 7.363761e-31 11 6479657 6479971 315 - 2.050 1.525 -1.775
ENSG00000132254 E018 1168.23982 2.192878e-04 4.571383e-06 3.283793e-05 11 6479972 6480068 97 - 3.022 2.988 -0.113
ENSG00000132254 E019 17.13888 2.044742e-03 3.433774e-11 6.545766e-10 11 6480069 6480109 41 - 1.524 0.998 -1.855
ENSG00000132254 E020 23.95531 1.290052e-02 9.210423e-14 2.632600e-12 11 6480110 6480322 213 - 1.712 1.089 -2.164
ENSG00000132254 E021 1080.84613 8.540286e-04 5.179928e-05 2.907017e-04 11 6480323 6480423 101 - 2.998 2.952 -0.154
ENSG00000132254 E022 313.24459 1.904307e-04 4.660352e-08 5.058380e-07 11 6480424 6480463 40 - 2.499 2.402 -0.325
ENSG00000132254 E023 43.22574 1.223819e-02 5.227837e-12 1.139436e-10 11 6480707 6481230 524 - 1.893 1.396 -1.693
ENSG00000132254 E024 765.76773 3.713501e-03 4.203379e-01 5.635453e-01 11 6481231 6481284 54 - 2.814 2.814 0.001
ENSG00000132254 E025 594.30843 3.986003e-03 6.160611e-01 7.342018e-01 11 6481285 6481479 195 - 2.698 2.706 0.026