ENSG00000131931

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254250 ENSG00000131931 HEK293_OSMI2_2hA HEK293_TMG_2hB THAP1 protein_coding protein_coding 5.177106 1.811098 9.05964 0.1076748 0.257086 2.316237 3.2347605 1.601621 4.864521 0.18070988 0.30524092 1.596748 0.70542917 0.8790333 0.5381667 -0.3408667 1.251480e-03 1.725175e-12 FALSE TRUE
ENST00000345117 ENSG00000131931 HEK293_OSMI2_2hA HEK293_TMG_2hB THAP1 protein_coding protein_coding 5.177106 1.811098 9.05964 0.1076748 0.257086 2.316237 0.2994749 0.185250 0.811024 0.07525028 0.08448492 2.072102 0.05225833 0.1069000 0.0892000 -0.0177000 9.009439e-01 1.725175e-12   FALSE
ENST00000529779 ENSG00000131931 HEK293_OSMI2_2hA HEK293_TMG_2hB THAP1 protein_coding protein_coding 5.177106 1.811098 9.05964 0.1076748 0.257086 2.316237 1.5988377 0.000000 3.283730 0.00000000 0.39850679 8.363578 0.23257917 0.0000000 0.3616333 0.3616333 1.725175e-12 1.725175e-12 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131931 E001 2.1454870 0.0067313248 0.3476704187 0.491861084 8 42836674 42836675 2 - 0.509 0.331 -0.964
ENSG00000131931 E002 124.4094955 0.0003066322 0.0473327713 0.104421030 8 42836676 42838144 1469 - 2.035 2.090 0.185
ENSG00000131931 E003 40.8564371 0.0011717346 0.9692674478 0.984789367 8 42838145 42838231 87 - 1.577 1.574 -0.009
ENSG00000131931 E004 40.9032087 0.0005366276 0.1365241214 0.243218375 8 42838232 42838336 105 - 1.553 1.636 0.283
ENSG00000131931 E005 21.2597032 0.0018536221 0.7938319514 0.868232071 8 42839186 42839207 22 - 1.308 1.285 -0.079
ENSG00000131931 E006 44.4991041 0.0005712640 0.8379298942 0.898672483 8 42839208 42839381 174 - 1.612 1.600 -0.040
ENSG00000131931 E007 0.9201396 0.0160719500 0.6498529530 0.760988332 8 42842488 42843023 536 - 0.285 0.196 -0.699
ENSG00000131931 E008 56.1967134 0.0007443461 0.0009964516 0.003945713 8 42843024 42843325 302 - 1.753 1.581 -0.586