ENSG00000131873

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254190 ENSG00000131873 HEK293_OSMI2_2hA HEK293_TMG_2hB CHSY1 protein_coding protein_coding 15.23502 10.40479 21.80322 1.038156 0.6862269 1.066569 12.404345 9.8876519 17.226050 1.0308317 0.7412967 0.800271 0.8395333 0.94980000 0.7895000 -0.1603000 5.678625e-07 5.678625e-07 FALSE TRUE
ENST00000543813 ENSG00000131873 HEK293_OSMI2_2hA HEK293_TMG_2hB CHSY1 protein_coding nonsense_mediated_decay 15.23502 10.40479 21.80322 1.038156 0.6862269 1.066569 2.479729 0.4322352 3.958818 0.1508857 0.2165547 3.165824 0.1380583 0.04263333 0.1825667 0.1399333 4.936363e-04 5.678625e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131873 E001 725.6446725 0.0051596236 6.674869e-23 5.865185e-21 15 101175727 101177100 1374 - 2.684 3.035 1.168
ENSG00000131873 E002 768.4566430 0.0001469800 2.167204e-06 1.672452e-05 15 101177101 101178576 1476 - 2.855 2.898 0.141
ENSG00000131873 E003 68.6695750 0.0006768239 9.828115e-04 3.900997e-03 15 101178577 101178601 25 - 1.847 1.794 -0.181
ENSG00000131873 E004 198.0002853 0.0023628344 6.851664e-08 7.183724e-07 15 101178602 101178980 379 - 2.313 2.220 -0.309
ENSG00000131873 E005 0.0000000       15 101186679 101186794 116 -      
ENSG00000131873 E006 0.1472490 0.0449809131 8.458787e-01   15 101187541 101187685 145 - 0.087 0.000 -8.878
ENSG00000131873 E007 0.9232720 0.0137599071 1.444355e-01 2.540586e-01 15 101187686 101187992 307 - 0.159 0.464 2.107
ENSG00000131873 E008 11.3047183 0.0015061745 6.194693e-01 7.368637e-01 15 101187993 101188219 227 - 1.057 1.096 0.141
ENSG00000131873 E009 0.0000000       15 101189428 101189567 140 -      
ENSG00000131873 E010 115.7340372 0.0009763631 2.568305e-08 2.931247e-07 15 101235082 101235254 173 - 2.087 1.974 -0.378
ENSG00000131873 E011 157.3619275 0.0004014636 5.866033e-17 2.592261e-15 15 101235255 101235508 254 - 2.234 2.072 -0.542
ENSG00000131873 E012 75.6514791 0.0004055483 1.590796e-09 2.272101e-08 15 101235509 101235577 69 - 1.921 1.752 -0.571
ENSG00000131873 E013 0.1451727 0.0433262778 8.463840e-01   15 101242548 101242617 70 - 0.087 0.000 -8.919
ENSG00000131873 E014 86.0170763 0.0003394790 7.096336e-25 7.690959e-23 15 101251137 101252069 933 - 2.021 1.667 -1.194