ENSG00000131828

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000422285 ENSG00000131828 HEK293_OSMI2_2hA HEK293_TMG_2hB PDHA1 protein_coding protein_coding 99.28718 112.5608 95.71951 2.859965 2.029009 -0.2337972 47.358489 65.217705 40.02523 5.089402 0.8178477 -0.70421493 0.4701792 0.57873333 0.4182000 -0.16053333 9.942719e-03 3.979478e-05 FALSE TRUE
ENST00000479146 ENSG00000131828 HEK293_OSMI2_2hA HEK293_TMG_2hB PDHA1 protein_coding retained_intron 99.28718 112.5608 95.71951 2.859965 2.029009 -0.2337972 7.864983 4.404624 10.95445 0.401122 1.4892826 1.31247077 0.0818375 0.03926667 0.1142333 0.07496667 3.979478e-05 3.979478e-05 FALSE FALSE
ENST00000492364 ENSG00000131828 HEK293_OSMI2_2hA HEK293_TMG_2hB PDHA1 protein_coding retained_intron 99.28718 112.5608 95.71951 2.859965 2.029009 -0.2337972 33.894608 33.240133 34.21141 5.168223 0.8613281 0.04153903 0.3437292 0.29600000 0.3574333 0.06143333 5.957219e-01 3.979478e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131828 E001 3.800849 4.295487e-03 1.071312e-02 3.048361e-02 X 19343893 19343919 27 + 0.872 0.507 -1.540
ENSG00000131828 E002 10.021530 1.764388e-03 1.633754e-03 6.069295e-03 X 19343920 19343926 7 + 1.205 0.884 -1.177
ENSG00000131828 E003 17.849644 2.836184e-03 1.640911e-05 1.037914e-04 X 19343927 19343935 9 + 1.455 1.100 -1.248
ENSG00000131828 E004 148.672374 2.095105e-04 1.663020e-20 1.129226e-18 X 19343936 19343962 27 + 2.313 2.042 -0.907
ENSG00000131828 E005 553.121936 1.834647e-03 1.382553e-04 6.958936e-04 X 19343963 19344094 132 + 2.782 2.679 -0.341
ENSG00000131828 E006 3.151950 2.932148e-02 6.565159e-01 7.660538e-01 X 19345736 19345819 84 + 0.647 0.570 -0.341
ENSG00000131828 E007 29.016246 2.702356e-03 1.205440e-01 2.207426e-01 X 19346510 19346623 114 + 1.527 1.424 -0.356
ENSG00000131828 E008 565.546887 3.540227e-03 2.249179e-01 3.563276e-01 X 19349312 19349371 60 + 2.755 2.708 -0.156
ENSG00000131828 E009 1164.767282 1.937291e-03 8.454732e-02 1.666691e-01 X 19349937 19350110 174 + 3.068 3.022 -0.154
ENSG00000131828 E010 10.486223 1.656102e-02 7.973718e-01 8.707012e-01 X 19351260 19351280 21 + 1.053 1.017 -0.134
ENSG00000131828 E011 1141.119753 1.661279e-03 1.466379e-01 2.571179e-01 X 19351281 19351407 127 + 3.054 3.017 -0.125
ENSG00000131828 E012 9.082847 4.899082e-02 3.044825e-04 1.397570e-03 X 19351408 19351498 91 + 1.260 0.718 -2.026
ENSG00000131828 E013 32.499645 8.801991e-03 7.046794e-19 3.957222e-17 X 19352829 19353081 253 + 1.833 1.147 -2.364
ENSG00000131828 E014 1006.202528 7.808237e-04 2.157205e-01 3.452559e-01 X 19353082 19353173 92 + 2.992 2.967 -0.084
ENSG00000131828 E015 27.377633 7.915041e-04 5.343582e-17 2.372453e-15 X 19353174 19353408 235 + 1.721 1.170 -1.903
ENSG00000131828 E016 824.145250 9.905251e-05 6.064321e-03 1.877940e-02 X 19354491 19354583 93 + 2.866 2.904 0.124
ENSG00000131828 E017 40.339116 1.340043e-03 1.825979e-07 1.763130e-06 X 19354951 19355348 398 + 1.768 1.474 -1.000
ENSG00000131828 E018 1412.238400 7.083812e-05 1.085849e-06 8.945119e-06 X 19355349 19355504 156 + 3.091 3.141 0.166
ENSG00000131828 E019 2.437820 5.954324e-03 1.937310e-03 7.032336e-03 X 19355582 19355685 104 + 0.789 0.298 -2.389
ENSG00000131828 E020 1155.385170 7.833640e-04 1.964958e-06 1.531096e-05 X 19355686 19355757 72 + 2.981 3.060 0.263
ENSG00000131828 E021 11.720748 1.543316e-03 7.846175e-02 1.570162e-01 X 19357382 19357651 270 + 1.194 1.025 -0.606
ENSG00000131828 E022 1221.475889 8.469894e-04 4.543017e-05 2.585887e-04 X 19357652 19357719 68 + 3.013 3.081 0.226
ENSG00000131828 E023 1499.508725 1.261968e-03 1.480760e-04 7.387499e-04 X 19358916 19359024 109 + 3.101 3.169 0.228
ENSG00000131828 E024 2422.537468 1.081274e-03 8.149130e-02 1.618896e-01 X 19359489 19361718 2230 + 3.375 3.347 -0.093