ENSG00000131781

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000254090 ENSG00000131781 HEK293_OSMI2_2hA HEK293_TMG_2hB FMO5 protein_coding protein_coding 0.4839276 0.3433527 0.7107444 0.03461781 0.07074854 1.028379 0.223571364 0.16278269 0.31903990 0.05619653 0.06116608 0.9293038 0.48678750 0.5185333 0.44790000 -0.07063333 0.926703340 0.001030077 FALSE TRUE
ENST00000369272 ENSG00000131781 HEK293_OSMI2_2hA HEK293_TMG_2hB FMO5 protein_coding protein_coding 0.4839276 0.3433527 0.7107444 0.03461781 0.07074854 1.028379 0.046205233 0.00000000 0.04228319 0.00000000 0.02585438 2.3863472 0.09381667 0.0000000 0.06826667 0.06826667 0.671968509 0.001030077 FALSE TRUE
ENST00000441068 ENSG00000131781 HEK293_OSMI2_2hA HEK293_TMG_2hB FMO5 protein_coding protein_coding 0.4839276 0.3433527 0.7107444 0.03461781 0.07074854 1.028379 0.062758325 0.00000000 0.22416812 0.00000000 0.01770032 4.5494728 0.11459167 0.0000000 0.32000000 0.32000000 0.001030077 0.001030077 FALSE TRUE
ENST00000527849 ENSG00000131781 HEK293_OSMI2_2hA HEK293_TMG_2hB FMO5 protein_coding nonsense_mediated_decay 0.4839276 0.3433527 0.7107444 0.03461781 0.07074854 1.028379 0.052565716 0.04566845 0.08245813 0.02499506 0.03506077 0.7319403 0.11664167 0.1281000 0.10913333 -0.01896667 0.883700661 0.001030077 TRUE TRUE
ENST00000578284 ENSG00000131781 HEK293_OSMI2_2hA HEK293_TMG_2hB FMO5 protein_coding protein_coding 0.4839276 0.3433527 0.7107444 0.03461781 0.07074854 1.028379 0.060874802 0.09949751 0.04279502 0.09949751 0.04279502 -1.0524244 0.12159583 0.2572667 0.05470000 -0.20256667 0.898303544 0.001030077 FALSE TRUE
ENST00000619062 ENSG00000131781 HEK293_OSMI2_2hA HEK293_TMG_2hB FMO5 protein_coding retained_intron 0.4839276 0.3433527 0.7107444 0.03461781 0.07074854 1.028379 0.007534156 0.03540408 0.00000000 0.03540408 0.00000000 -2.1828220 0.01817917 0.0961000 0.00000000 -0.09610000 0.577734869 0.001030077 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131781 E001 0.0000000       1 147175351 147176390 1040 -      
ENSG00000131781 E002 1.0330470 0.012167424 0.022479415 0.05668310 1 147184305 147184491 187 - 0.439 0.000 -13.249
ENSG00000131781 E003 2.2520647 0.006884141 0.006411899 0.01969159 1 147184492 147184630 139 - 0.653 0.153 -3.048
ENSG00000131781 E004 8.6443217 0.005117639 0.748948995 0.83603984 1 147186261 147187245 985 - 1.000 0.956 -0.164
ENSG00000131781 E005 2.9623993 0.005616853 0.006077998 0.01881622 1 147190177 147190249 73 - 0.741 0.266 -2.411
ENSG00000131781 E006 2.5142077 0.012073321 0.252431194 0.38878365 1 147201152 147201343 192 - 0.628 0.430 -0.945
ENSG00000131781 E007 3.5920891 0.005067097 0.856940596 0.91158866 1 147201344 147201504 161 - 0.677 0.642 -0.148
ENSG00000131781 E008 0.2214452 0.040908795 0.282103612   1 147208439 147208851 413 - 0.000 0.154 11.692
ENSG00000131781 E009 6.0119487 0.003169546 0.463964172 0.60371682 1 147208852 147209051 200 - 0.813 0.890 0.299
ENSG00000131781 E010 3.6922355 0.004825710 0.923581016 0.95585186 1 147212393 147212446 54 - 0.677 0.682 0.021
ENSG00000131781 E011 4.3845846 0.004195250 0.483642044 0.62168063 1 147212447 147212535 89 - 0.699 0.784 0.343
ENSG00000131781 E012 3.0268386 0.005749132 0.331074147 0.47467007 1 147213308 147213358 51 - 0.544 0.682 0.607
ENSG00000131781 E013 3.6827578 0.004648165 0.792619396 0.86740665 1 147213359 147213470 112 - 0.653 0.682 0.122
ENSG00000131781 E014 0.0000000       1 147214782 147215753 972 -      
ENSG00000131781 E015 4.5934315 0.005046415 0.479536383 0.61791248 1 147215754 147215942 189 - 0.699 0.784 0.346
ENSG00000131781 E016 5.8950645 0.003615506 0.756764692 0.84180896 1 147224895 147225066 172 - 0.813 0.841 0.109
ENSG00000131781 E017 0.0000000       1 147225067 147225276 210 -      
ENSG00000131781 E018 0.0000000       1 147225277 147225286 10 -      
ENSG00000131781 E019 1.4340170 0.149321830 0.202868774 0.32981387 1 147225287 147225573 287 - 0.243 0.500 1.528
ENSG00000131781 E020 0.7761102 0.077620106 0.128415635 0.23195015 1 147225574 147225638 65 - 0.097 0.359 2.360
ENSG00000131781 E021 0.1451727 0.043579257 0.817628220   1 147225729 147225873 145 - 0.097 0.000 -10.442
ENSG00000131781 E022 3.4805260 0.135913088 0.310802812 0.45323496 1 147242813 147243050 238 - 0.545 0.731 0.804