ENSG00000131653

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000326181 ENSG00000131653 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAF7 protein_coding protein_coding 45.99544 62.09373 38.708 5.620727 0.5786943 -0.6816754 28.526573 44.647893 15.584583 2.722191 0.4358668 -1.5178702 0.5987583 0.72306667 0.4030333 -0.32003333 1.918613e-12 1.918613e-12 FALSE TRUE
ENST00000569686 ENSG00000131653 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAF7 protein_coding retained_intron 45.99544 62.09373 38.708 5.620727 0.5786943 -0.6816754 2.151449 1.534893 5.464462 1.534893 1.2891216 1.8252105 0.0503000 0.02146667 0.1408333 0.11936667 7.576955e-02 1.918613e-12 FALSE FALSE
ENST00000570169 ENSG00000131653 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAF7 protein_coding retained_intron 45.99544 62.09373 38.708 5.620727 0.5786943 -0.6816754 4.307301 3.009336 6.467378 0.798548 0.5275845 1.1011784 0.1033125 0.04703333 0.1667333 0.11970000 2.378188e-07 1.918613e-12 FALSE FALSE
MSTRG.11844.4 ENSG00000131653 HEK293_OSMI2_2hA HEK293_TMG_2hB TRAF7 protein_coding   45.99544 62.09373 38.708 5.620727 0.5786943 -0.6816754 6.392238 7.202369 7.958643 1.664361 0.3438051 0.1438608 0.1434375 0.11760000 0.2057667 0.08816667 1.187725e-01 1.918613e-12 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131653 E001 0.658951 0.0217331589 8.477086e-01 9.054336e-01 16 2155503 2155571 69 + 0.176 0.214 0.349
ENSG00000131653 E002 5.416453 0.0093177399 3.632415e-01 5.076740e-01 16 2155698 2155777 80 + 0.844 0.720 -0.492
ENSG00000131653 E003 5.093740 0.0482056608 9.368628e-01 9.643087e-01 16 2155778 2155781 4 + 0.739 0.737 -0.009
ENSG00000131653 E004 66.572066 0.0003917902 3.376824e-01 4.815751e-01 16 2155782 2155803 22 + 1.798 1.757 -0.139
ENSG00000131653 E005 125.169740 0.0007510587 9.094652e-01 9.464911e-01 16 2155804 2155828 25 + 2.036 2.045 0.030
ENSG00000131653 E006 148.299879 0.0003895898 4.108072e-01 5.543966e-01 16 2155829 2155858 30 + 2.132 2.110 -0.075
ENSG00000131653 E007 1.001723 0.0124415477 3.029578e-01 4.448803e-01 16 2163542 2163799 258 + 0.397 0.214 -1.230
ENSG00000131653 E008 272.362899 0.0001624037 7.208038e-04 2.973321e-03 16 2163883 2164001 119 + 2.431 2.356 -0.251
ENSG00000131653 E009 13.266557 0.0129496961 6.351443e-02 1.325485e-01 16 2165857 2165878 22 + 1.229 1.035 -0.698
ENSG00000131653 E010 258.177379 0.0001586855 5.681054e-05 3.156369e-04 16 2165879 2165936 58 + 2.419 2.326 -0.308
ENSG00000131653 E011 335.690245 0.0001568795 1.339635e-06 1.082629e-05 16 2168077 2168168 92 + 2.536 2.438 -0.326
ENSG00000131653 E012 33.375333 0.0027410293 4.747630e-03 1.522616e-02 16 2168169 2168515 347 + 1.300 1.521 0.765
ENSG00000131653 E013 332.030752 0.0015926698 7.271178e-04 2.996395e-03 16 2170614 2170730 117 + 2.524 2.437 -0.290
ENSG00000131653 E014 310.836101 0.0037588191 3.144810e-02 7.478125e-02 16 2171264 2171356 93 + 2.484 2.416 -0.227
ENSG00000131653 E015 3.221490 0.2916817805 1.115981e-01 2.079025e-01 16 2171357 2171382 26 + 0.778 0.456 -1.429
ENSG00000131653 E016 222.665049 0.0075989066 7.296461e-03 2.199177e-02 16 2171572 2171605 34 + 2.375 2.256 -0.396
ENSG00000131653 E017 451.217523 0.0012026558 8.930826e-04 3.590049e-03 16 2172191 2172374 184 + 2.648 2.576 -0.242
ENSG00000131653 E018 496.795368 0.0002462535 1.229697e-03 4.741465e-03 16 2172465 2172593 129 + 2.678 2.623 -0.181
ENSG00000131653 E019 232.057750 0.0016968750 2.468781e-02 6.124094e-02 16 2172594 2172599 6 + 2.357 2.291 -0.221
ENSG00000131653 E020 686.851091 0.0004472295 3.477031e-05 2.034144e-04 16 2173182 2173399 218 + 2.826 2.761 -0.217
ENSG00000131653 E021 306.852813 0.0001675704 3.301434e-03 1.115316e-02 16 2173481 2173512 32 + 2.473 2.412 -0.204
ENSG00000131653 E022 344.921675 0.0004909018 2.305168e-03 8.167941e-03 16 2173513 2173554 42 + 2.524 2.460 -0.214
ENSG00000131653 E023 355.635587 0.0021727774 9.913105e-03 2.854810e-02 16 2173788 2173836 49 + 2.541 2.473 -0.226
ENSG00000131653 E024 491.797372 0.0008666903 4.693474e-03 1.507800e-02 16 2173921 2174048 128 + 2.672 2.617 -0.183
ENSG00000131653 E025 367.029549 0.0006602029 6.847456e-01 7.876703e-01 16 2174251 2174333 83 + 2.493 2.509 0.053
ENSG00000131653 E026 299.963455 0.0002770394 3.629856e-01 5.074095e-01 16 2175111 2175150 40 + 2.396 2.423 0.090
ENSG00000131653 E027 8.907102 0.0241802298 2.728929e-04 1.269366e-03 16 2175151 2175219 69 + 1.229 0.771 -1.701
ENSG00000131653 E028 509.631204 0.0001120737 2.226001e-02 5.621215e-02 16 2175301 2175417 117 + 2.613 2.659 0.153
ENSG00000131653 E029 604.543537 0.0001083732 1.009689e-01 1.919486e-01 16 2175500 2175622 123 + 2.697 2.729 0.106
ENSG00000131653 E030 663.871996 0.0001467052 3.503810e-02 8.172393e-02 16 2175834 2175953 120 + 2.734 2.773 0.130
ENSG00000131653 E031 626.208608 0.0002063882 1.695888e-03 6.270307e-03 16 2176049 2176180 132 + 2.694 2.752 0.195
ENSG00000131653 E032 433.291341 0.0020205521 6.704305e-07 5.779271e-06 16 2176265 2176384 120 + 2.462 2.616 0.510
ENSG00000131653 E033 453.900399 0.0036973894 3.242967e-07 2.979738e-06 16 2176560 2176787 228 + 2.453 2.643 0.633
ENSG00000131653 E034 556.098639 0.0027919659 4.669081e-05 2.650000e-04 16 2176788 2177323 536 + 2.584 2.719 0.449
ENSG00000131653 E035 199.860956 0.0009736446 3.233200e-06 2.401851e-05 16 2177324 2177436 113 + 2.116 2.276 0.537
ENSG00000131653 E036 614.055667 0.0013589217 2.331801e-14 7.264473e-13 16 2177437 2178635 1199 + 2.580 2.774 0.646