ENSG00000131584

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000354700 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding protein_coding 30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 9.686472 15.8556747 5.184047 1.33598313 0.15288198 -1.61097786 0.32987917 0.55990000 0.24023333 -0.31966667 6.849668e-15 6.849668e-15 FALSE TRUE
ENST00000467278 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding retained_intron 30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 2.762544 1.5714205 1.871405 0.45629454 0.28071934 0.25058964 0.08490417 0.05313333 0.08556667 0.03243333 2.319771e-01 6.849668e-15 FALSE TRUE
ENST00000470659 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding retained_intron 30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 2.569125 1.3457491 2.618458 0.10753094 0.79939543 0.95512666 0.08494167 0.04793333 0.11636667 0.06843333 6.639491e-02 6.849668e-15 FALSE FALSE
ENST00000472541 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding retained_intron 30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 2.310182 1.2200503 1.710625 0.34881660 0.17094158 0.48421525 0.07347083 0.04263333 0.07923333 0.03660000 2.163301e-01 6.849668e-15 FALSE TRUE
ENST00000478065 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding retained_intron 30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 1.688249 0.8311843 1.333685 0.05605124 0.09964227 0.67570163 0.05615000 0.02946667 0.06120000 0.03173333 7.622505e-04 6.849668e-15   FALSE
ENST00000493992 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding retained_intron 30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 3.478088 1.4779509 2.856055 0.13171250 0.29034803 0.94573959 0.11599167 0.05213333 0.13110000 0.07896667 1.804571e-07 6.849668e-15   FALSE
MSTRG.91.19 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding   30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 2.530342 1.9475128 3.143667 0.49513338 0.43781084 0.68800868 0.08751667 0.06623333 0.14553333 0.07930000 1.368961e-02 6.849668e-15 FALSE TRUE
MSTRG.91.9 ENSG00000131584 HEK293_OSMI2_2hA HEK293_TMG_2hB ACAP3 protein_coding   30.06067 28.51621 21.76804 3.249251 1.497926 -0.389414 1.953098 1.7586050 1.785385 0.15527808 0.24133260 0.02168124 0.06450000 0.06223333 0.08136667 0.01913333 3.188690e-01 6.849668e-15 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131584 E001 1.544645 0.0089687092 1.498164e-01 2.614310e-01 1 1292390 1292390 1 - 0.170 0.443 1.892
ENSG00000131584 E002 209.861702 0.0087831313 1.106408e-06 9.098260e-06 1 1292391 1292869 479 - 2.066 2.335 0.899
ENSG00000131584 E003 378.281428 0.0001479355 9.880470e-17 4.251250e-15 1 1292870 1293563 694 - 2.390 2.566 0.586
ENSG00000131584 E004 28.286833 0.0008143205 2.031623e-02 5.214747e-02 1 1293564 1293567 4 - 1.271 1.449 0.617
ENSG00000131584 E005 89.271661 0.0031224126 1.624684e-02 4.326885e-02 1 1293568 1293708 141 - 1.803 1.929 0.425
ENSG00000131584 E006 87.912765 0.0028120495 5.212697e-02 1.129470e-01 1 1293823 1293933 111 - 1.816 1.917 0.340
ENSG00000131584 E007 76.738040 0.0003903822 4.519869e-01 5.928860e-01 1 1294090 1294199 110 - 1.855 1.823 -0.109
ENSG00000131584 E008 64.189123 0.0006091621 1.842738e-04 8.961655e-04 1 1294402 1294498 97 - 1.875 1.702 -0.585
ENSG00000131584 E009 114.235566 0.0043770828 1.012356e-01 1.923514e-01 1 1294499 1294628 130 - 2.054 1.986 -0.229
ENSG00000131584 E010 107.206261 0.0002828136 6.102739e-01 7.296191e-01 1 1294718 1294816 99 - 1.965 1.984 0.066
ENSG00000131584 E011 14.411778 0.0163733360 2.284420e-05 1.397180e-04 1 1294817 1295008 192 - 1.412 0.965 -1.595
ENSG00000131584 E012 168.556549 0.0029881449 6.861732e-02 1.410718e-01 1 1295447 1295554 108 - 2.120 2.198 0.260
ENSG00000131584 E013 207.864255 0.0006264477 1.097971e-01 2.052650e-01 1 1295736 1295938 203 - 2.229 2.278 0.162
ENSG00000131584 E014 118.625808 0.0003174057 3.326611e-01 4.763382e-01 1 1296015 1296109 95 - 1.995 2.031 0.120
ENSG00000131584 E015 92.143178 0.0023707758 7.123577e-01 8.091303e-01 1 1296211 1296280 70 - 1.899 1.919 0.067
ENSG00000131584 E016 52.383747 0.0038843168 4.368797e-01 5.789096e-01 1 1296425 1296435 11 - 1.638 1.688 0.167
ENSG00000131584 E017 76.314058 0.0005572526 6.452752e-01 7.574115e-01 1 1296436 1296465 30 - 1.816 1.837 0.073
ENSG00000131584 E018 165.805227 0.0002376387 9.900262e-03 2.851631e-02 1 1296466 1296633 168 - 2.106 2.186 0.271
ENSG00000131584 E019 73.798490 0.0003799977 1.431554e-01 2.523153e-01 1 1297822 1297836 15 - 1.769 1.837 0.229
ENSG00000131584 E020 127.788080 0.0002860370 9.836907e-02 1.880326e-01 1 1297837 1297933 97 - 2.014 2.072 0.196
ENSG00000131584 E021 124.616385 0.0002988682 2.218883e-01 3.526500e-01 1 1298013 1298113 101 - 2.012 2.055 0.147
ENSG00000131584 E022 16.847313 0.0011166499 1.993482e-06 1.551217e-05 1 1298114 1298184 71 - 1.443 1.050 -1.389
ENSG00000131584 E023 35.197002 0.0006670142 2.923891e-09 3.976756e-08 1 1298185 1298369 185 - 1.732 1.382 -1.199
ENSG00000131584 E024 93.384072 0.0003105930 1.792246e-01 3.001552e-01 1 1298370 1298421 52 - 1.881 1.936 0.186
ENSG00000131584 E025 126.794719 0.0003128186 8.837298e-03 2.587475e-02 1 1298567 1298679 113 - 1.980 2.075 0.317
ENSG00000131584 E026 14.715219 0.0084624376 3.210937e-04 1.465330e-03 1 1298680 1298743 64 - 1.361 1.015 -1.231
ENSG00000131584 E027 63.267223 0.0090087847 5.313508e-09 6.899438e-08 1 1298744 1299344 601 - 1.982 1.622 -1.216
ENSG00000131584 E028 70.527029 0.0003956898 2.968029e-02 7.133362e-02 1 1299345 1299356 12 - 1.725 1.828 0.351
ENSG00000131584 E029 55.762364 0.0091683820 1.493937e-04 7.444026e-04 1 1299357 1299830 474 - 1.861 1.619 -0.817
ENSG00000131584 E030 107.333264 0.0003031153 3.510505e-01 4.952524e-01 1 1299831 1299896 66 - 1.956 1.991 0.120
ENSG00000131584 E031 73.086447 0.0004117197 7.788660e-01 8.577415e-01 1 1299897 1299905 9 - 1.810 1.823 0.044
ENSG00000131584 E032 119.956211 0.0031880140 3.756417e-01 5.200502e-01 1 1299973 1300068 96 - 2.003 2.044 0.136
ENSG00000131584 E033 96.931330 0.0002966761 2.320918e-01 3.649282e-01 1 1300158 1300195 38 - 1.902 1.950 0.162
ENSG00000131584 E034 73.649887 0.0003440520 2.496390e-01 3.854923e-01 1 1300196 1300202 7 - 1.780 1.833 0.179
ENSG00000131584 E035 175.041275 0.0001948612 7.254727e-01 8.187352e-01 1 1300509 1300692 184 - 2.183 2.194 0.036
ENSG00000131584 E036 32.678094 0.0022914785 4.516189e-10 7.131009e-09 1 1300747 1300930 184 - 1.721 1.322 -1.366
ENSG00000131584 E037 11.833797 0.0039264639 2.594689e-07 2.431727e-06 1 1301448 1301855 408 - 1.361 0.858 -1.822
ENSG00000131584 E038 11.979664 0.0014559059 2.120227e-09 2.955417e-08 1 1301856 1301987 132 - 1.387 0.816 -2.076
ENSG00000131584 E039 103.581444 0.0003605354 2.643570e-01 4.023172e-01 1 1301988 1302046 59 - 1.991 1.949 -0.139
ENSG00000131584 E040 84.693003 0.0003697646 1.165312e-01 2.149939e-01 1 1302922 1302975 54 - 1.922 1.858 -0.217
ENSG00000131584 E041 80.635142 0.0005952339 5.437751e-02 1.169128e-01 1 1303162 1303209 48 - 1.915 1.833 -0.275
ENSG00000131584 E042 81.214279 0.0019295017 1.401872e-02 3.826862e-02 1 1303210 1303281 72 - 1.939 1.828 -0.373
ENSG00000131584 E043 37.420833 0.0075993037 1.570838e-08 1.866473e-07 1 1303282 1304085 804 - 1.783 1.389 -1.347
ENSG00000131584 E044 81.524993 0.0003484515 5.672788e-06 3.987728e-05 1 1304086 1304143 58 - 1.987 1.802 -0.621
ENSG00000131584 E045 53.832209 0.0038989352 1.240805e-08 1.504042e-07 1 1304144 1305929 1786 - 1.891 1.572 -1.080
ENSG00000131584 E046 7.552424 0.0023146266 3.958548e-01 5.396381e-01 1 1307072 1307156 85 - 0.960 0.858 -0.386
ENSG00000131584 E047 5.222763 0.0076058399 3.572130e-05 2.083526e-04 1 1307337 1307383 47 - 1.061 0.508 -2.241
ENSG00000131584 E048 48.546626 0.0011663290 2.573879e-07 2.414287e-06 1 1307769 1307930 162 - 1.819 1.547 -0.924
ENSG00000131584 E049 2.611177 0.0674974814 1.283046e-01 2.317836e-01 1 1309442 1309609 168 - 0.683 0.412 -1.269