ENSG00000131473

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000352035 ENSG00000131473 HEK293_OSMI2_2hA HEK293_TMG_2hB ACLY protein_coding protein_coding 221.0191 287.3284 160.9303 18.50866 2.088343 -0.8362251 52.57033 48.74853 68.14600 2.389623 4.727911 0.4831861 0.2563458 0.1702667 0.4231000 0.2528333 2.347647e-13 4.021901e-92 FALSE TRUE
ENST00000353196 ENSG00000131473 HEK293_OSMI2_2hA HEK293_TMG_2hB ACLY protein_coding protein_coding 221.0191 287.3284 160.9303 18.50866 2.088343 -0.8362251 70.08025 115.69551 17.66026 6.788889 3.530033 -2.7110628 0.2956125 0.4033667 0.1102000 -0.2931667 9.304485e-07 4.021901e-92 FALSE TRUE
ENST00000393896 ENSG00000131473 HEK293_OSMI2_2hA HEK293_TMG_2hB ACLY protein_coding protein_coding 221.0191 287.3284 160.9303 18.50866 2.088343 -0.8362251 27.53730 0.00000 51.08311 0.000000 4.795314 12.3189129 0.1497292 0.0000000 0.3168667 0.3168667 4.021901e-92 4.021901e-92 FALSE TRUE
ENST00000590151 ENSG00000131473 HEK293_OSMI2_2hA HEK293_TMG_2hB ACLY protein_coding protein_coding 221.0191 287.3284 160.9303 18.50866 2.088343 -0.8362251 59.97166 112.81018 17.34352 11.100166 4.811231 -2.7007248 0.2481958 0.3909333 0.1082333 -0.2827000 3.407059e-04 4.021901e-92 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131473 E001 3.6438524 4.635249e-03 1.850263e-03 6.759097e-03 17 41866867 41866916 50 - 0.164 0.750 3.334
ENSG00000131473 E002 3.4621481 6.851894e-03 2.791884e-03 9.649345e-03 17 41866917 41866917 1 - 0.164 0.731 3.259
ENSG00000131473 E003 84.0035741 2.745659e-02 6.206861e-04 2.609045e-03 17 41866918 41866930 13 - 1.611 1.972 1.219
ENSG00000131473 E004 4422.4468717 6.671373e-03 2.937189e-20 1.932760e-18 17 41866931 41867455 525 - 3.303 3.688 1.279
ENSG00000131473 E005 2005.7704267 2.319072e-03 6.470034e-34 1.462683e-31 17 41867456 41867573 118 - 3.006 3.337 1.099
ENSG00000131473 E006 2738.3313576 1.600248e-03 2.315434e-29 3.667659e-27 17 41867574 41867760 187 - 3.204 3.459 0.847
ENSG00000131473 E007 2939.9212066 1.145086e-03 6.728837e-22 5.298246e-20 17 41867761 41867904 144 - 3.285 3.477 0.637
ENSG00000131473 E008 2479.4994131 1.153609e-03 1.471115e-12 3.507405e-11 17 41868709 41868785 77 - 3.245 3.394 0.494
ENSG00000131473 E009 2402.9203457 7.614881e-04 7.607814e-07 6.486105e-06 17 41869043 41869125 83 - 3.271 3.367 0.320
ENSG00000131473 E010 2511.1613046 7.370468e-04 1.528716e-06 1.220082e-05 17 41869474 41869587 114 - 3.293 3.386 0.309
ENSG00000131473 E011 12.9801747 1.416712e-02 6.577981e-04 2.743920e-03 17 41870355 41870719 365 - 1.316 0.971 -1.240
ENSG00000131473 E012 2728.0184137 7.198237e-04 3.105613e-05 1.841526e-04 17 41871689 41871832 144 - 3.337 3.419 0.271
ENSG00000131473 E013 2434.6510357 4.852089e-04 5.058374e-03 1.609009e-02 17 41872032 41872182 151 - 3.307 3.363 0.188
ENSG00000131473 E014 2256.7646426 1.772304e-04 9.794639e-01 9.912083e-01 17 41873811 41873965 155 - 3.299 3.320 0.068
ENSG00000131473 E015 3.1844172 1.369981e-01 4.153973e-01 5.587919e-01 17 41873966 41873998 33 - 0.708 0.525 -0.807
ENSG00000131473 E016 1775.0212692 6.586420e-05 2.226804e-01 3.535841e-01 17 41878103 41878196 94 - 3.204 3.213 0.029
ENSG00000131473 E017 1752.9658361 1.233034e-04 6.414631e-02 1.336052e-01 17 41878797 41878924 128 - 3.176 3.216 0.133
ENSG00000131473 E018 1621.2687065 7.469750e-05 9.424122e-02 1.817583e-01 17 41883122 41883232 111 - 3.144 3.182 0.126
ENSG00000131473 E019 1513.4386283 9.363012e-05 6.139747e-03 1.897792e-02 17 41884193 41884274 82 - 3.147 3.138 -0.029
ENSG00000131473 E020 2318.8636564 5.076673e-05 7.521701e-08 7.823453e-07 17 41886112 41886308 197 - 3.343 3.319 -0.081
ENSG00000131473 E021 1900.3514032 5.128941e-04 5.004228e-09 6.530522e-08 17 41887599 41887703 105 - 3.281 3.220 -0.203
ENSG00000131473 E022 1942.1466176 2.363477e-04 1.089974e-10 1.912143e-09 17 41892279 41892447 169 - 3.285 3.233 -0.172
ENSG00000131473 E023 1548.0234183 4.430050e-04 4.283567e-05 2.452429e-04 17 41893033 41893174 142 - 3.176 3.139 -0.124
ENSG00000131473 E024 495.5718608 1.207473e-04 1.433832e-02 3.899890e-02 17 41896620 41896649 30 - 2.673 2.650 -0.074
ENSG00000131473 E025 969.6367245 9.822336e-05 7.270595e-04 2.996220e-03 17 41897749 41897800 52 - 2.964 2.941 -0.076
ENSG00000131473 E026 947.4537754 1.759011e-04 5.267436e-10 8.217903e-09 17 41897801 41897839 39 - 2.982 2.918 -0.213
ENSG00000131473 E027 1688.4480057 2.014575e-04 1.703010e-12 4.016310e-11 17 41898631 41898785 155 - 3.229 3.169 -0.198
ENSG00000131473 E028 1484.0363401 1.715644e-03 2.023274e-08 2.356903e-07 17 41901696 41901813 118 - 3.203 3.098 -0.348
ENSG00000131473 E029 3.0235161 1.474916e-01 2.762837e-01 4.157244e-01 17 41904440 41904728 289 - 0.709 0.534 -0.765
ENSG00000131473 E030 1082.0867566 1.984827e-03 8.769914e-09 1.092761e-07 17 41904729 41904790 62 - 3.076 2.956 -0.399
ENSG00000131473 E031 1378.3136069 1.721758e-03 2.525291e-09 3.475545e-08 17 41905522 41905638 117 - 3.176 3.063 -0.378
ENSG00000131473 E032 713.4141132 1.700031e-03 2.957901e-06 2.215934e-05 17 41905639 41905658 20 - 2.878 2.783 -0.315
ENSG00000131473 E033 1145.0283057 2.094441e-03 5.243805e-08 5.629736e-07 17 41906528 41906597 70 - 3.097 2.982 -0.382
ENSG00000131473 E034 1088.9953545 2.837056e-03 7.618037e-08 7.915396e-07 17 41906598 41906646 49 - 3.086 2.954 -0.437
ENSG00000131473 E035 1481.0975654 3.114359e-03 9.449160e-07 7.879374e-06 17 41907442 41907572 131 - 3.211 3.092 -0.397
ENSG00000131473 E036 1145.8502595 3.845289e-03 1.187027e-05 7.753435e-05 17 41908989 41909068 80 - 3.100 2.980 -0.398
ENSG00000131473 E037 1753.2447593 4.096828e-03 2.242979e-06 1.725866e-05 17 41909510 41909700 191 - 3.292 3.161 -0.436
ENSG00000131473 E038 786.1521054 3.116629e-03 2.489696e-09 3.430294e-08 17 41910222 41910238 17 - 2.963 2.802 -0.535
ENSG00000131473 E039 886.2008928 1.552196e-03 2.352217e-12 5.432495e-11 17 41910239 41910284 46 - 3.002 2.862 -0.466
ENSG00000131473 E040 1442.2390916 2.412527e-03 5.928996e-14 1.740903e-12 17 41912420 41912542 123 - 3.234 3.061 -0.574
ENSG00000131473 E041 1603.8882351 3.785079e-03 1.947885e-13 5.315008e-12 17 41913715 41913896 182 - 3.301 3.093 -0.689
ENSG00000131473 E042 307.3129138 5.582939e-03 3.362579e-12 7.571977e-11 17 41913897 41913900 4 - 2.622 2.350 -0.906
ENSG00000131473 E043 817.5978114 5.746618e-03 3.456146e-11 6.585762e-10 17 41918880 41919022 143 - 3.027 2.787 -0.798
ENSG00000131473 E044 0.6277178 2.465124e-02 8.682684e-02 1.703139e-01 17 41919638 41919662 25 - 0.376 0.090 -2.571
ENSG00000131473 E045 33.3669834 6.817014e-04 1.486355e-03 5.592340e-03 17 41930358 41930489 132 - 1.611 1.434 -0.609
ENSG00000131473 E046 16.9948648 2.930951e-03 4.857612e-01 6.235796e-01 17 41930490 41930542 53 - 1.243 1.199 -0.154