ENSG00000131370

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000383791 ENSG00000131370 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3BP5 protein_coding protein_coding 11.70757 10.58754 11.43332 0.619604 0.5903388 0.110777 6.915386 7.666870 6.809314 1.0514097 0.1932368 -0.1708917 0.5948125 0.7176000 0.6003667 -0.1172333 4.246714e-01 9.269528e-28 FALSE TRUE
ENST00000408919 ENSG00000131370 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3BP5 protein_coding protein_coding 11.70757 10.58754 11.43332 0.619604 0.5903388 0.110777 1.991477 0.000000 3.043903 0.0000000 0.4051740 8.2545106 0.1610208 0.0000000 0.2641000 0.2641000 9.269528e-28 9.269528e-28 FALSE TRUE
ENST00000412806 ENSG00000131370 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3BP5 protein_coding nonsense_mediated_decay 11.70757 10.58754 11.43332 0.619604 0.5903388 0.110777 2.628890 2.866261 1.503129 0.4595413 0.4138959 -0.9266597 0.2297333 0.2769667 0.1287333 -0.1482333 1.157507e-01 9.269528e-28 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131370 E001 45.7369224 0.0194277030 4.807445e-07 4.266036e-06 3 15254353 15255003 651 - 1.348 1.811 1.583
ENSG00000131370 E002 210.8320028 0.0049132510 9.104000e-09 1.130968e-07 3 15255004 15255588 585 - 2.174 2.409 0.785
ENSG00000131370 E003 93.1744372 0.0022566939 5.917000e-03 1.839143e-02 3 15255589 15255649 61 - 1.892 2.021 0.433
ENSG00000131370 E004 314.1511399 0.0002410225 8.259856e-01 8.904801e-01 3 15255650 15256303 654 - 2.487 2.508 0.070
ENSG00000131370 E005 139.7815467 0.0002759526 9.161922e-02 1.776650e-01 3 15256853 15257113 261 - 2.166 2.134 -0.106
ENSG00000131370 E006 56.0779745 0.0007570699 2.353911e-01 3.688300e-01 3 15258831 15258864 34 - 1.779 1.740 -0.131
ENSG00000131370 E007 50.3132373 0.0005089619 7.265146e-02 1.477558e-01 3 15258865 15258883 19 - 1.748 1.677 -0.239
ENSG00000131370 E008 106.9958445 0.0004274653 1.954894e-02 5.051611e-02 3 15258884 15259050 167 - 2.070 2.009 -0.204
ENSG00000131370 E009 55.2050158 0.0004594457 4.341818e-01 5.765743e-01 3 15259761 15259803 43 - 1.763 1.745 -0.063
ENSG00000131370 E010 1.1351654 0.0140758250 7.637534e-02 1.537040e-01 3 15262158 15262158 1 - 0.128 0.454 2.427
ENSG00000131370 E011 39.0929900 0.0005699421 5.231958e-01 6.569296e-01 3 15262159 15262160 2 - 1.615 1.597 -0.060
ENSG00000131370 E012 99.7496048 0.0020309838 1.492310e-03 5.611513e-03 3 15262161 15262289 129 - 2.065 1.958 -0.358
ENSG00000131370 E013 88.6866418 0.0003783148 1.409071e-03 5.336965e-03 3 15269713 15269842 130 - 2.010 1.911 -0.333
ENSG00000131370 E014 53.9214946 0.0017766991 2.244550e-01 3.557550e-01 3 15269843 15269877 35 - 1.766 1.722 -0.149
ENSG00000131370 E015 0.0000000       3 15271601 15271671 71 -      
ENSG00000131370 E016 0.1482932 0.0406322727 5.496535e-01   3 15273116 15273405 290 - 0.000 0.116 11.374
ENSG00000131370 E017 0.0000000       3 15275668 15276045 378 -      
ENSG00000131370 E018 0.0000000       3 15304102 15304102 1 -      
ENSG00000131370 E019 89.3257500 0.0038984723 5.351376e-02 1.154014e-01 3 15304103 15304231 129 - 1.997 1.927 -0.232
ENSG00000131370 E020 0.2944980 0.3548894463 2.532780e-01   3 15304232 15304299 68 - 0.227 0.000 -11.803
ENSG00000131370 E021 61.4676749 0.0047490001 9.182872e-02 1.779766e-01 3 15330504 15330566 63 - 1.837 1.762 -0.254
ENSG00000131370 E022 0.1451727 0.0438129273 4.359413e-01   3 15331784 15332270 487 - 0.128 0.000 -12.198
ENSG00000131370 E023 63.8567885 0.0113115556 1.057986e-01 1.993101e-01 3 15332271 15332586 316 - 1.860 1.762 -0.329
ENSG00000131370 E024 0.0000000       3 15333088 15333189 102 -      
ENSG00000131370 E025 0.2214452 0.0534973291 5.447876e-01   3 15341223 15341368 146 - 0.000 0.117 11.249