ENSG00000131196

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253506 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.2126276 0.1960356 0.41052550 0.1960356 0.21174259 1.029300 0.06115000 0.03533333 0.19803333 0.162700000 4.670424e-01 8.861819e-06 FALSE TRUE
ENST00000318065 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.8357515 1.5089871 0.65134374 0.1984540 0.13342581 -1.199637 0.28824167 0.32270000 0.30240000 -0.020300000 9.554051e-01 8.861819e-06 FALSE TRUE
ENST00000329101 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.2846889 0.0000000 0.22952335 0.0000000 0.22952335 4.582094 0.10263333 0.00000000 0.11096667 0.110966667 7.184241e-01 8.861819e-06 FALSE TRUE
ENST00000427363 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.2438648 0.0000000 0.46151131 0.0000000 0.23353781 5.559220 0.10394583 0.00000000 0.21386667 0.213866667 1.076227e-01 8.861819e-06 FALSE TRUE
ENST00000542384 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.3582032 0.9767234 0.00000000 0.2190463 0.00000000 -6.624574 0.09350833 0.21450000 0.00000000 -0.214500000 8.861819e-06 8.861819e-06 FALSE TRUE
ENST00000545796 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.1077606 0.4322466 0.00000000 0.2162796 0.00000000 -5.466779 0.02210000 0.08390000 0.00000000 -0.083900000 2.692963e-01 8.861819e-06 FALSE TRUE
ENST00000586434 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.1561233 0.0000000 0.00000000 0.0000000 0.00000000 0.000000 0.05473750 0.00000000 0.00000000 0.000000000   8.861819e-06 FALSE TRUE
ENST00000590172 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding processed_transcript 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.2544464 0.4120010 0.15952813 0.4120010 0.15952813 -1.315722 0.06789167 0.07423333 0.07056667 -0.003666667 9.608339e-01 8.861819e-06   FALSE
ENST00000591814 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.2134114 0.3097198 0.10793884 0.3097198 0.05770498 -1.438769 0.05342500 0.06423333 0.04940000 -0.014833333 7.118521e-01 8.861819e-06 FALSE TRUE
ENST00000592223 ENSG00000131196 HEK293_OSMI2_2hA HEK293_TMG_2hB NFATC1 protein_coding protein_coding 3.11257 4.753835 2.135976 0.4800461 0.06224971 -1.15049 0.1794590 0.4821462 0.08783738 0.3106812 0.08783738 -2.330629 0.05437917 0.09670000 0.04246667 -0.054233333 7.016099e-01 8.861819e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131196 E001 0.6590396 0.0190801093 6.821528e-02 1.404068e-01 18 79395856 79395929 74 + 0.396 0.084 -2.805
ENSG00000131196 E002 0.6590396 0.0190801093 6.821528e-02 1.404068e-01 18 79395930 79395943 14 + 0.396 0.084 -2.805
ENSG00000131196 E003 2.5337741 0.0187666619 3.981823e-04 1.767318e-03 18 79395944 79396130 187 + 0.843 0.268 -2.804
ENSG00000131196 E004 4.4805241 0.0047349138 2.054772e-02 5.264608e-02 18 79396131 79396224 94 + 0.901 0.577 -1.328
ENSG00000131196 E005 8.1705525 0.0039498376 1.546502e-02 4.154863e-02 18 79396225 79396351 127 + 1.095 0.816 -1.045
ENSG00000131196 E006 0.3332198 0.0301563771 5.369875e-01   18 79400274 79400321 48 + 0.175 0.084 -1.223
ENSG00000131196 E007 1.2544010 0.0107689928 3.339938e-01 4.777568e-01 18 79400322 79400330 9 + 0.175 0.358 1.363
ENSG00000131196 E008 1.2544010 0.0107689928 3.339938e-01 4.777568e-01 18 79400331 79400332 2 + 0.175 0.358 1.363
ENSG00000131196 E009 1.5542096 0.0096166748 6.012868e-01 7.224113e-01 18 79400333 79400335 3 + 0.300 0.396 0.584
ENSG00000131196 E010 3.2367417 0.0051089872 5.349818e-01 6.669477e-01 18 79400336 79400391 56 + 0.651 0.551 -0.444
ENSG00000131196 E011 5.1491381 0.0031959066 2.547826e-01 3.915651e-01 18 79400392 79400479 88 + 0.843 0.685 -0.636
ENSG00000131196 E012 94.6027233 0.0004567028 5.008199e-04 2.163202e-03 18 79410403 79411501 1099 + 2.010 1.884 -0.426
ENSG00000131196 E013 23.1317414 0.0084521866 6.167504e-01 7.347413e-01 18 79433579 79433738 160 + 1.299 1.343 0.155
ENSG00000131196 E014 0.0000000       18 79448270 79448401 132 +      
ENSG00000131196 E015 0.2214452 0.0385907490 1.000000e+00   18 79448572 79448781 210 + 0.000 0.084 10.079
ENSG00000131196 E016 34.4033797 0.0006105532 2.106059e-01 3.392542e-01 18 79448782 79448984 203 + 1.429 1.510 0.277
ENSG00000131196 E017 28.3798875 0.0007707755 4.196557e-01 5.629094e-01 18 79450954 79451041 88 + 1.369 1.424 0.193
ENSG00000131196 E018 20.3932121 0.0140045732 9.451470e-01 9.695959e-01 18 79451042 79451078 37 + 1.276 1.277 0.003
ENSG00000131196 E019 20.5257981 0.0087985536 8.633170e-01 9.158313e-01 18 79451079 79451126 48 + 1.265 1.278 0.048
ENSG00000131196 E020 33.4196235 0.0082473299 6.146376e-02 1.290952e-01 18 79451676 79451816 141 + 1.368 1.514 0.502
ENSG00000131196 E021 0.1482932 0.0423469053 1.000000e+00   18 79451817 79452131 315 + 0.000 0.084 10.059
ENSG00000131196 E022 30.1205686 0.0043824593 1.014371e-01 1.926550e-01 18 79461311 79461366 56 + 1.340 1.465 0.433
ENSG00000131196 E023 46.1880655 0.0005132941 2.258796e-02 5.689395e-02 18 79467450 79467582 133 + 1.516 1.646 0.443
ENSG00000131196 E024 43.9512226 0.0014294534 2.979271e-07 2.759431e-06 18 79467583 79470014 2432 + 1.320 1.661 1.171
ENSG00000131196 E025 24.3729880 0.0008362930 1.288769e-02 3.565272e-02 18 79486248 79486630 383 + 1.475 1.295 -0.621
ENSG00000131196 E026 7.2666247 0.0042046746 1.794330e-03 6.581424e-03 18 79486631 79486937 307 + 1.095 0.722 -1.422
ENSG00000131196 E027 0.1515154 0.0442282370 2.083614e-01   18 79493396 79493823 428 + 0.175 0.000 -12.601
ENSG00000131196 E028 0.1482932 0.0423469053 1.000000e+00   18 79523924 79524035 112 + 0.000 0.084 10.059
ENSG00000131196 E029 0.2214452 0.0385907490 1.000000e+00   18 79527119 79527402 284 + 0.000 0.084 10.079
ENSG00000131196 E030 156.2686958 0.0124783467 7.632367e-01 8.466168e-01 18 79527528 79529325 1798 + 2.146 2.137 -0.029