ENSG00000131153

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253462 ENSG00000131153 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS2 protein_coding protein_coding 163.915 255.622 124.4971 30.91231 3.029519 -1.03784 21.65134 34.17898 15.46145 4.455117 0.3687074 -1.1439235 0.1314750 0.1338333 0.1242000 -0.009633333 0.705727393 0.002083043 FALSE  
MSTRG.13169.2 ENSG00000131153 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS2 protein_coding   163.915 255.622 124.4971 30.91231 3.029519 -1.03784 76.91746 129.99972 53.91706 15.394000 0.4816646 -1.2695382 0.4561375 0.5091667 0.4333667 -0.075800000 0.002083043 0.002083043 FALSE  
MSTRG.13169.3 ENSG00000131153 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS2 protein_coding   163.915 255.622 124.4971 30.91231 3.029519 -1.03784 30.24149 42.94100 24.99745 5.626968 0.1311977 -0.7803341 0.1908500 0.1683000 0.2010000 0.032700000 0.212666943 0.002083043 FALSE  
MSTRG.13169.7 ENSG00000131153 HEK293_OSMI2_2hA HEK293_TMG_2hB GINS2 protein_coding   163.915 255.622 124.4971 30.91231 3.029519 -1.03784 34.84908 48.30422 29.84711 10.083611 2.1966158 -0.6943733 0.2197042 0.1879333 0.2391667 0.051233333 0.418821166 0.002083043 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131153 E001 756.887940 6.746858e-04 3.313870e-01 4.749923e-01 16 85676198 85677806 1609 - 2.805 2.832 0.091
ENSG00000131153 E002 590.595635 1.381418e-03 1.289217e-05 8.347176e-05 16 85677807 85677821 15 - 2.628 2.743 0.386
ENSG00000131153 E003 3378.985681 8.439843e-04 1.845958e-05 1.154543e-04 16 85677822 85678337 516 - 3.427 3.484 0.187
ENSG00000131153 E004 1106.703981 1.116662e-04 9.453837e-01 9.697074e-01 16 85678540 85678569 30 - 2.984 2.994 0.033
ENSG00000131153 E005 1284.203335 1.444479e-04 4.469567e-02 9.964298e-02 16 85678570 85678622 53 - 3.065 3.052 -0.043
ENSG00000131153 E006 1288.322334 8.658292e-05 7.438940e-06 5.088773e-05 16 85678623 85678666 44 - 3.086 3.047 -0.128
ENSG00000131153 E007 2.839846 5.496415e-03 9.314091e-01 9.607885e-01 16 85678735 85678914 180 - 0.549 0.544 -0.026
ENSG00000131153 E008 1559.438401 2.855572e-04 6.679554e-03 2.039029e-02 16 85681582 85681681 100 - 3.156 3.131 -0.083
ENSG00000131153 E009 0.000000       16 85683745 85683816 72 -      
ENSG00000131153 E010 1200.145678 1.747401e-03 7.633612e-02 1.536414e-01 16 85687460 85687574 115 - 3.048 3.011 -0.121
ENSG00000131153 E011 3.167958 5.924635e-02 1.566246e-01 2.706070e-01 16 85687713 85688025 313 - 0.746 0.490 -1.130
ENSG00000131153 E012 872.244000 4.526432e-03 1.066938e-01 2.006694e-01 16 85688809 85689013 205 - 2.922 2.867 -0.182
ENSG00000131153 E013 1.325377 9.978179e-03 5.862623e-01 7.098614e-01 16 85689796 85690073 278 - 0.402 0.310 -0.552