ENSG00000131069

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000360596 ENSG00000131069 HEK293_OSMI2_2hA HEK293_TMG_2hB ACSS2 protein_coding protein_coding 20.50613 27.435 15.07528 0.9940854 0.3176899 -0.8634024 12.1117479 17.2555430 7.3425855 0.7245054 0.003188016 -1.231572 0.57959167 0.62876667 0.487500000 -0.14126667 1.189445e-05 1.189445e-05 FALSE TRUE
ENST00000477932 ENSG00000131069 HEK293_OSMI2_2hA HEK293_TMG_2hB ACSS2 protein_coding nonsense_mediated_decay 20.50613 27.435 15.07528 0.9940854 0.3176899 -0.8634024 1.2777627 3.8409573 0.1255041 0.3391203 0.125504095 -4.828809 0.05222083 0.14090000 0.008433333 -0.13246667 1.259323e-02 1.189445e-05 FALSE TRUE
ENST00000480274 ENSG00000131069 HEK293_OSMI2_2hA HEK293_TMG_2hB ACSS2 protein_coding nonsense_mediated_decay 20.50613 27.435 15.07528 0.9940854 0.3176899 -0.8634024 0.8101985 0.6056439 1.4241610 0.4448422 0.486148530 1.220039 0.04692083 0.02220000 0.093333333 0.07113333 2.974107e-01 1.189445e-05   FALSE
ENST00000490464 ENSG00000131069 HEK293_OSMI2_2hA HEK293_TMG_2hB ACSS2 protein_coding retained_intron 20.50613 27.435 15.07528 0.9940854 0.3176899 -0.8634024 1.7840849 0.7181018 2.2946591 0.3532230 0.328543450 1.662341 0.09720833 0.02543333 0.153233333 0.12780000 1.178102e-03 1.189445e-05   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131069 E001 0.7718438 0.0386112144 9.405479e-01 9.666590e-01 20 34872146 34872403 258 + 0.182 0.208 0.241
ENSG00000131069 E002 0.0000000       20 34872882 34873001 120 +      
ENSG00000131069 E003 0.2214452 0.0388349520 1.000000e+00   20 34874942 34874962 21 + 0.000 0.081 12.659
ENSG00000131069 E004 0.2214452 0.0388349520 1.000000e+00   20 34874963 34874978 16 + 0.000 0.081 12.659
ENSG00000131069 E005 0.2214452 0.0388349520 1.000000e+00   20 34874979 34875016 38 + 0.000 0.081 12.659
ENSG00000131069 E006 0.2214452 0.0388349520 1.000000e+00   20 34875017 34875127 111 + 0.000 0.081 12.659
ENSG00000131069 E007 0.0000000       20 34875128 34875137 10 +      
ENSG00000131069 E008 0.0000000       20 34875853 34875931 79 +      
ENSG00000131069 E009 2.9859639 0.0670577727 3.847263e-01 5.288668e-01 20 34876572 34876579 8 + 0.667 0.531 -0.602
ENSG00000131069 E010 5.6882147 0.0449500682 1.656916e-01 2.825972e-01 20 34876580 34876589 10 + 0.919 0.719 -0.783
ENSG00000131069 E011 6.9321083 0.0036400186 6.662063e-02 1.377682e-01 20 34876590 34876592 3 + 0.993 0.785 -0.796
ENSG00000131069 E012 23.5610794 0.0138335009 5.464512e-01 6.766265e-01 20 34876593 34876611 19 + 1.349 1.316 -0.115
ENSG00000131069 E013 25.5068845 0.0151678160 7.580479e-01 8.427118e-01 20 34876612 34876613 2 + 1.359 1.354 -0.015
ENSG00000131069 E014 26.5430494 0.0118035099 7.194172e-01 8.142566e-01 20 34876614 34876621 8 + 1.378 1.369 -0.030
ENSG00000131069 E015 59.7929925 0.0217184291 5.387623e-01 6.701392e-01 20 34876622 34876747 126 + 1.732 1.711 -0.073
ENSG00000131069 E016 57.9871291 0.0239581687 9.376716e-01 9.648057e-01 20 34876748 34876823 76 + 1.684 1.709 0.084
ENSG00000131069 E017 51.8242316 0.0202458999 5.664700e-01 6.934499e-01 20 34882794 34882817 24 + 1.669 1.649 -0.070
ENSG00000131069 E018 44.0394214 0.0152337683 2.225832e-01 3.534789e-01 20 34882818 34882826 9 + 1.640 1.564 -0.258
ENSG00000131069 E019 123.3556212 0.0012312415 1.371023e-02 3.756235e-02 20 34882827 34882989 163 + 2.078 2.007 -0.238
ENSG00000131069 E020 0.2987644 0.0273034674 4.286982e-02   20 34882990 34882996 7 + 0.310 0.000 -16.619
ENSG00000131069 E021 0.9650892 0.0313040146 7.374233e-01 8.276056e-01 20 34893569 34893622 54 + 0.310 0.258 -0.357
ENSG00000131069 E022 0.4439371 0.0215668095 1.022734e-02 2.932510e-02 20 34908716 34908903 188 + 0.408 0.000 -17.209
ENSG00000131069 E023 5.0091768 0.0032428203 1.322765e-01 2.372779e-01 20 34908905 34908943 39 + 0.860 0.669 -0.767
ENSG00000131069 E024 0.1472490 0.0430276932 1.771998e-01   20 34913093 34913095 3 + 0.182 0.000 -15.597
ENSG00000131069 E025 97.7145478 0.0003456879 1.695045e-04 8.329561e-04 20 34913096 34913181 86 + 2.017 1.893 -0.415
ENSG00000131069 E026 57.1543783 0.0004555526 3.991849e-04 1.771270e-03 20 34913182 34913187 6 + 1.813 1.659 -0.520
ENSG00000131069 E027 0.0000000       20 34913188 34913188 1 +      
ENSG00000131069 E028 77.0196140 0.0003608335 1.375500e-03 5.226740e-03 20 34913393 34913440 48 + 1.912 1.795 -0.394
ENSG00000131069 E029 66.5992039 0.0018463376 1.402946e-02 3.829133e-02 20 34913441 34913496 56 + 1.836 1.735 -0.343
ENSG00000131069 E030 0.8179419 0.0166350527 1.287248e-01 2.323787e-01 20 34913497 34913503 7 + 0.408 0.148 -1.937
ENSG00000131069 E031 82.9580015 0.0003866749 9.394971e-01 9.660230e-01 20 34913753 34913825 73 + 1.840 1.858 0.063
ENSG00000131069 E032 1.4255980 0.0771567293 5.653519e-01 6.924693e-01 20 34913854 34913902 49 + 0.408 0.308 -0.595
ENSG00000131069 E033 3.7849503 0.0042988768 3.517288e-01 4.959545e-01 20 34913903 34914095 193 + 0.713 0.586 -0.544
ENSG00000131069 E034 63.8514627 0.0003858087 6.154175e-01 7.336933e-01 20 34914096 34914117 22 + 1.745 1.742 -0.010
ENSG00000131069 E035 63.8558809 0.0008712801 1.792162e-01 3.001492e-01 20 34914118 34914171 54 + 1.670 1.763 0.314
ENSG00000131069 E036 86.1985274 0.0003523141 9.526561e-01 9.743414e-01 20 34914323 34914437 115 + 1.856 1.875 0.065
ENSG00000131069 E037 0.0000000       20 34915218 34915256 39 +      
ENSG00000131069 E038 112.9930388 0.0003011980 5.577614e-01 6.861142e-01 20 34919435 34919572 138 + 1.988 1.988 0.000
ENSG00000131069 E039 0.4772466 0.0205311943 8.037602e-01 8.751399e-01 20 34920532 34920538 7 + 0.182 0.148 -0.354
ENSG00000131069 E040 148.8318245 0.0009260531 8.260319e-01 8.905096e-01 20 34920539 34920709 171 + 2.081 2.112 0.102
ENSG00000131069 E041 4.8446318 0.0249980981 5.545780e-06 3.908018e-05 20 34920949 34921005 57 + 1.095 0.452 -2.644
ENSG00000131069 E042 152.4520348 0.0022075412 8.898617e-01 9.336082e-01 20 34921006 34921139 134 + 2.094 2.123 0.096
ENSG00000131069 E043 134.9111272 0.0002354184 5.723657e-01 6.984950e-01 20 34921330 34921462 133 + 2.062 2.065 0.009
ENSG00000131069 E044 91.9399757 0.0003337553 7.648358e-01 8.478064e-01 20 34921544 34921600 57 + 1.895 1.904 0.032
ENSG00000131069 E045 3.4967739 0.0060763026 4.636543e-04 2.020703e-03 20 34921648 34921785 138 + 0.918 0.420 -2.159
ENSG00000131069 E046 102.5172828 0.0031102803 7.602107e-01 8.443829e-01 20 34921786 34921866 81 + 1.923 1.961 0.128
ENSG00000131069 E047 5.8990257 0.0027871866 2.805847e-02 6.812933e-02 20 34921963 34922366 404 + 0.969 0.705 -1.031
ENSG00000131069 E048 6.4204659 0.0044949267 8.565971e-02 1.684483e-01 20 34922842 34923322 481 + 0.944 0.740 -0.797
ENSG00000131069 E049 128.5732466 0.0026848854 3.829246e-01 5.270855e-01 20 34923323 34923431 109 + 2.001 2.062 0.205
ENSG00000131069 E050 112.5415607 0.0003474292 5.553966e-02 1.189027e-01 20 34925698 34925766 69 + 1.912 2.009 0.327
ENSG00000131069 E051 160.6611703 0.0031422424 1.359100e-01 2.423682e-01 20 34926105 34926281 177 + 2.076 2.162 0.289
ENSG00000131069 E052 129.2475377 0.0048680896 8.548871e-01 9.102373e-01 20 34926877 34926951 75 + 2.026 2.059 0.114
ENSG00000131069 E053 489.6757939 0.0089672668 4.389794e-04 1.924542e-03 20 34927087 34927962 876 + 2.482 2.663 0.602