ENSG00000131013

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253329 ENSG00000131013 HEK293_OSMI2_2hA HEK293_TMG_2hB PPIL4 protein_coding protein_coding 25.95439 8.913909 39.7915 1.284783 1.200951 2.157075 8.228047 5.171544 9.918838 0.286933 0.5148487 0.9382427 0.3801292 0.5961667 0.2490000 -0.3471667 2.199120e-05 9.439467e-06 FALSE TRUE
MSTRG.29136.2 ENSG00000131013 HEK293_OSMI2_2hA HEK293_TMG_2hB PPIL4 protein_coding   25.95439 8.913909 39.7915 1.284783 1.200951 2.157075 17.425943 3.628535 29.322757 1.056647 0.7422209 3.0110830 0.6081167 0.3909000 0.7371667 0.3462667 9.439467e-06 9.439467e-06 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000131013 E001 103.259450 0.0089837787 2.776483e-20 1.830364e-18 6 149504495 149505274 780 - 1.749 2.288 1.809
ENSG00000131013 E002 314.361325 0.0014963537 9.820013e-06 6.541393e-05 6 149505275 149505704 430 - 2.392 2.531 0.465
ENSG00000131013 E003 196.207805 0.0034483050 5.174330e-01 6.518171e-01 6 149512155 149512302 148 - 2.212 2.263 0.170
ENSG00000131013 E004 164.040479 0.0006423065 9.982792e-01 1.000000e+00 6 149517354 149517450 97 - 2.142 2.162 0.068
ENSG00000131013 E005 3.673386 0.0098036016 2.556034e-01 3.925110e-01 6 149517451 149517864 414 - 0.655 0.462 -0.893
ENSG00000131013 E006 189.848362 0.0001811267 1.791676e-01 3.001049e-01 6 149521060 149521171 112 - 2.217 2.196 -0.070
ENSG00000131013 E007 159.325999 0.0003528636 7.324934e-01 8.239393e-01 6 149525143 149525209 67 - 2.134 2.142 0.027
ENSG00000131013 E008 204.738948 0.0017546270 4.500811e-02 1.002169e-01 6 149526652 149526776 125 - 2.255 2.207 -0.162
ENSG00000131013 E009 206.977775 0.0010477803 4.245120e-04 1.868336e-03 6 149533458 149533574 117 - 2.269 2.174 -0.316
ENSG00000131013 E010 151.673406 0.0002107919 1.455833e-02 3.949582e-02 6 149534678 149534774 97 - 2.131 2.067 -0.216
ENSG00000131013 E011 175.412423 0.0002192451 1.979144e-02 5.104529e-02 6 149535596 149535738 143 - 2.191 2.136 -0.183
ENSG00000131013 E012 158.202647 0.0002976495 2.769810e-02 6.741656e-02 6 149540942 149541059 118 - 2.147 2.092 -0.184
ENSG00000131013 E013 110.180521 0.0003171588 1.784008e-02 4.679410e-02 6 149541367 149541429 63 - 1.997 1.919 -0.260
ENSG00000131013 E014 54.377631 0.0004228633 2.529714e-02 6.248688e-02 6 149541430 149541431 2 - 1.702 1.590 -0.381
ENSG00000131013 E015 89.691043 0.0002948357 2.863732e-02 6.928275e-02 6 149541519 149541586 68 - 1.908 1.828 -0.268
ENSG00000131013 E016 75.531998 0.0003556936 1.874044e-01 3.104657e-01 6 149545936 149546078 143 - 1.826 1.781 -0.151