ENSG00000130939

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253251 ENSG00000130939 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE4B protein_coding protein_coding 22.98575 28.4922 19.29762 0.953961 0.08973973 -0.5619026 12.8600030 9.421088 13.7420149 2.3211328 0.24911309 0.5441469 0.57527917 0.3302333 0.7122333 0.38200000 5.686004e-04 6.25311e-11 FALSE TRUE
ENST00000343090 ENSG00000130939 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE4B protein_coding protein_coding 22.98575 28.4922 19.29762 0.953961 0.08973973 -0.5619026 3.6517975 6.310659 2.6437807 0.2181135 0.22752098 -1.2520257 0.15318333 0.2215667 0.1369333 -0.08463333 1.782406e-03 6.25311e-11 FALSE TRUE
ENST00000487244 ENSG00000130939 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE4B protein_coding retained_intron 22.98575 28.4922 19.29762 0.953961 0.08973973 -0.5619026 4.7463506 11.944083 0.7022421 2.1255155 0.09824152 -4.0689920 0.19009167 0.4192000 0.0364000 -0.38280000 6.253110e-11 6.25311e-11 FALSE TRUE
MSTRG.272.1 ENSG00000130939 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE4B protein_coding   22.98575 28.4922 19.29762 0.953961 0.08973973 -0.5619026 0.9359097 0.000000 1.1763207 0.0000000 0.58842719 6.8903503 0.04594583 0.0000000 0.0607000 0.06070000 1.463187e-01 6.25311e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130939 E001 0.3289534 0.0290928297 8.902083e-01   1 10032832 10032957 126 + 0.128 0.117 -0.154
ENSG00000130939 E002 90.3147354 0.0022449956 4.208597e-09 5.553817e-08 1 10032958 10033273 316 + 2.066 1.847 -0.735
ENSG00000130939 E003 71.1254122 0.0003724693 3.451646e-13 9.090839e-12 1 10033274 10033297 24 + 1.986 1.715 -0.912
ENSG00000130939 E004 95.7332139 0.0015017265 1.038536e-11 2.156374e-10 1 10033298 10033358 61 + 2.100 1.863 -0.796
ENSG00000130939 E005 157.7105798 0.0032869458 9.470563e-11 1.677463e-09 1 10033359 10033577 219 + 2.308 2.093 -0.720
ENSG00000130939 E006 111.1497758 0.0067364408 3.727353e-06 2.733147e-05 1 10033578 10033694 117 + 2.150 1.952 -0.665
ENSG00000130939 E007 157.8941153 0.0032821785 8.150929e-14 2.347118e-12 1 10072028 10072214 187 + 2.326 2.072 -0.848
ENSG00000130939 E008 3.4131501 0.0060449135 8.319611e-01 8.947031e-01 1 10072454 10073118 665 + 0.647 0.643 -0.017
ENSG00000130939 E009 163.1639990 0.0078062664 3.227023e-09 4.353103e-08 1 10095461 10095596 136 + 2.342 2.083 -0.864
ENSG00000130939 E010 1.6950167 0.0190291121 9.799445e-01 9.914815e-01 1 10100861 10101107 247 + 0.434 0.454 0.103
ENSG00000130939 E011 124.6260262 0.0004117828 3.548017e-17 1.611511e-15 1 10101108 10101195 88 + 2.218 1.982 -0.790
ENSG00000130939 E012 139.6873764 0.0007490708 2.619655e-09 3.594971e-08 1 10102948 10103092 145 + 2.226 2.072 -0.516
ENSG00000130939 E013 0.1472490 0.0433006723 4.135115e-01   1 10103093 10103185 93 + 0.128 0.000 -10.454
ENSG00000130939 E014 171.8998006 0.0100092877 1.520956e-03 5.704625e-03 1 10105516 10105744 229 + 2.305 2.169 -0.455
ENSG00000130939 E015 18.9342219 0.0015504811 1.488851e-02 4.023772e-02 1 10106197 10106583 387 + 1.374 1.216 -0.554
ENSG00000130939 E016 3.1376566 0.0151378796 2.071336e-01 3.350883e-01 1 10107222 10107374 153 + 0.709 0.539 -0.742
ENSG00000130939 E017 112.1474087 0.0052564624 4.676361e-04 2.036229e-03 1 10117459 10117600 142 + 2.118 1.986 -0.441
ENSG00000130939 E018 107.4859857 0.0021130078 5.360597e-06 3.789080e-05 1 10119513 10119613 101 + 2.108 1.961 -0.493
ENSG00000130939 E019 101.2356220 0.0051633790 2.657376e-03 9.245255e-03 1 10121962 10122041 80 + 2.066 1.955 -0.371
ENSG00000130939 E020 78.4245829 0.0079144254 1.263752e-02 3.505637e-02 1 10122042 10122076 35 + 1.955 1.847 -0.363
ENSG00000130939 E021 122.2572053 0.0107582373 7.405192e-02 1.500098e-01 1 10126794 10126877 84 + 2.122 2.059 -0.212
ENSG00000130939 E022 0.9566832 0.0128015965 5.596307e-01 6.877160e-01 1 10128339 10129391 1053 + 0.227 0.349 0.844
ENSG00000130939 E023 105.1458216 0.0192085443 1.268659e-01 2.297850e-01 1 10129392 10129448 57 + 2.057 1.992 -0.217
ENSG00000130939 E024 129.0737123 0.0075899904 2.054579e-01 3.329801e-01 1 10130500 10130616 117 + 2.123 2.096 -0.091
ENSG00000130939 E025 105.0749329 0.0010098586 9.600781e-01 9.789089e-01 1 10130715 10130813 99 + 1.998 2.035 0.125
ENSG00000130939 E026 100.1619739 0.0062381587 8.134362e-01 8.817047e-01 1 10132369 10132482 114 + 1.988 2.015 0.092
ENSG00000130939 E027 189.5099248 0.0078450346 8.453002e-02 1.666437e-01 1 10134988 10135186 199 + 2.301 2.258 -0.142
ENSG00000130939 E028 157.8326094 0.0004005696 5.195657e-02 1.126525e-01 1 10137067 10137205 139 + 2.210 2.189 -0.068
ENSG00000130939 E029 132.1746774 0.0002564180 8.790086e-01 9.262843e-01 1 10144940 10145039 100 + 2.095 2.136 0.135
ENSG00000130939 E030 4.9984670 0.0048234313 5.689189e-01 6.955342e-01 1 10145040 10145599 560 + 0.709 0.816 0.431
ENSG00000130939 E031 124.1838352 0.0002556928 2.110683e-01 3.397909e-01 1 10146963 10147090 128 + 2.049 2.125 0.255
ENSG00000130939 E032 118.6813951 0.0059600329 2.847224e-01 4.249671e-01 1 10149184 10149282 99 + 2.020 2.106 0.289
ENSG00000130939 E033 253.1129436 0.0002156493 1.994697e-02 5.137771e-02 1 10151326 10151561 236 + 2.347 2.436 0.298
ENSG00000130939 E034 0.3332198 0.0291662941 8.895547e-01   1 10158354 10158355 2 + 0.128 0.117 -0.154
ENSG00000130939 E035 221.0488430 0.0001675759 2.929210e-04 1.350946e-03 1 10158356 10158482 127 + 2.267 2.391 0.414
ENSG00000130939 E036 241.0280380 0.0001794487 1.232547e-04 6.287530e-04 1 10161142 10161286 145 + 2.304 2.429 0.420
ENSG00000130939 E037 1.7359880 0.2107045560 2.673639e-01 4.056777e-01 1 10166244 10166342 99 + 0.227 0.513 1.718
ENSG00000130939 E038 214.6889436 0.0003602780 1.091201e-01 2.042503e-01 1 10168136 10168270 135 + 2.285 2.362 0.255
ENSG00000130939 E039 5.5965629 0.1175095343 8.448683e-01 9.034695e-01 1 10170956 10171137 182 + 0.813 0.815 0.007
ENSG00000130939 E040 264.1701860 0.0002304089 4.519912e-03 1.459989e-02 1 10171138 10171329 192 + 2.360 2.460 0.333
ENSG00000130939 E041 236.3775274 0.0045794155 3.386011e-04 1.534970e-03 1 10178644 10178818 175 + 2.261 2.437 0.585
ENSG00000130939 E042 0.3697384 0.0254377673 2.540368e-01 3.906753e-01 1 10179016 10179167 152 + 0.000 0.209 10.871
ENSG00000130939 E043 235.7119448 0.0055077488 1.144220e-04 5.881674e-04 1 10179416 10179562 147 + 2.245 2.442 0.657
ENSG00000130939 E044 224.6118004 0.0072359561 5.005575e-04 2.162235e-03 1 10179895 10180003 109 + 2.223 2.422 0.663
ENSG00000130939 E045 861.4184052 0.0088446117 3.211813e-12 7.255765e-11 1 10180004 10181175 1172 + 2.711 3.044 1.106
ENSG00000130939 E046 55.6305194 0.0162616186 1.711356e-12 4.034988e-11 1 10181176 10181239 64 + 1.283 1.922 2.182