ENSG00000130821

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253122 ENSG00000130821 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC6A8 protein_coding protein_coding 109.7483 164.6156 76.08795 18.24022 1.30891 -1.113259 12.166632 11.702587 14.002925 3.426809 0.6152373 0.2586984 0.11389583 0.06886667 0.18390000 0.115033333 4.220888e-03 7.463066e-09 FALSE TRUE
ENST00000430077 ENSG00000130821 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC6A8 protein_coding protein_coding 109.7483 164.6156 76.08795 18.24022 1.30891 -1.113259 43.136768 91.833308 19.524240 3.825389 0.6086716 -2.2331695 0.35945000 0.56750000 0.25683333 -0.310666667 7.029643e-07 7.463066e-09 FALSE TRUE
ENST00000442457 ENSG00000130821 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC6A8 protein_coding protein_coding 109.7483 164.6156 76.08795 18.24022 1.30891 -1.113259 10.814459 14.870293 6.924059 5.097345 0.5794977 -1.1016310 0.09783750 0.08533333 0.09100000 0.005666667 8.964873e-01 7.463066e-09 FALSE FALSE
ENST00000466243 ENSG00000130821 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC6A8 protein_coding processed_transcript 109.7483 164.6156 76.08795 18.24022 1.30891 -1.113259 15.968920 11.971645 16.681541 3.382374 0.5319404 0.4782911 0.16080417 0.06986667 0.21943333 0.149566667 2.442340e-04 7.463066e-09 FALSE FALSE
ENST00000476466 ENSG00000130821 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC6A8 protein_coding processed_transcript 109.7483 164.6156 76.08795 18.24022 1.30891 -1.113259 10.980153 10.430446 8.139988 2.282874 0.8792521 -0.3573135 0.10818750 0.06183333 0.10670000 0.044866667 7.252102e-02 7.463066e-09 TRUE FALSE
ENST00000675713 ENSG00000130821 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC6A8 protein_coding retained_intron 109.7483 164.6156 76.08795 18.24022 1.30891 -1.113259 7.459306 5.751167 6.744659 1.052413 0.5072017 0.2295219 0.07767083 0.03443333 0.08856667 0.054133333 7.463066e-09 7.463066e-09 FALSE FALSE
MSTRG.35074.9 ENSG00000130821 HEK293_OSMI2_2hA HEK293_TMG_2hB SLC6A8 protein_coding   109.7483 164.6156 76.08795 18.24022 1.30891 -1.113259 3.692187 10.912417 1.173352 1.773601 0.1119411 -3.2063408 0.03003750 0.06863333 0.01540000 -0.053233333 9.049338e-05 7.463066e-09 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130821 E001 3.425800 0.0047511789 3.824165e-06 2.794510e-05 X 153687926 153687938 13 + 0.994 0.332 -2.953
ENSG00000130821 E002 16.559588 0.0011638524 2.491724e-25 2.808148e-23 X 153687939 153687971 33 + 1.646 0.790 -3.067
ENSG00000130821 E003 16.559588 0.0011638524 2.491724e-25 2.808148e-23 X 153687972 153687972 1 + 1.646 0.790 -3.067
ENSG00000130821 E004 181.411281 0.0206250251 1.033908e-14 3.393464e-13 X 153687973 153688722 750 + 2.537 1.982 -1.855
ENSG00000130821 E005 374.451658 0.0019773218 2.672223e-08 3.037954e-07 X 153688723 153688836 114 + 2.597 2.453 -0.481
ENSG00000130821 E006 9.948640 0.0089343842 2.974856e-04 1.369502e-03 X 153689467 153689599 133 + 1.233 0.841 -1.439
ENSG00000130821 E007 9.574687 0.0025337095 2.605292e-08 2.969703e-07 X 153690359 153690374 16 + 1.310 0.732 -2.141
ENSG00000130821 E008 825.074894 0.0008739378 3.892306e-05 2.249979e-04 X 153690375 153690506 132 + 2.880 2.818 -0.207
ENSG00000130821 E009 51.813860 0.0049893914 5.827995e-13 1.484998e-11 X 153690507 153690652 146 + 1.919 1.503 -1.412
ENSG00000130821 E010 32.838744 0.0021736393 3.979078e-09 5.279622e-08 X 153690804 153690881 78 + 1.702 1.328 -1.282
ENSG00000130821 E011 50.162224 0.0045294154 3.370465e-15 1.190167e-13 X 153691118 153691303 186 + 1.928 1.475 -1.537
ENSG00000130821 E012 750.762479 0.0004535609 3.181159e-05 1.881082e-04 X 153691304 153691362 59 + 2.834 2.777 -0.189
ENSG00000130821 E013 596.087444 0.0006070633 8.578995e-05 4.553583e-04 X 153691363 153691392 30 + 2.739 2.676 -0.207
ENSG00000130821 E014 888.477254 0.0017069635 8.935454e-04 3.591604e-03 X 153691393 153691460 68 + 2.911 2.852 -0.196
ENSG00000130821 E015 882.665545 0.0023903204 2.248732e-04 1.069805e-03 X 153691461 153691507 47 + 2.923 2.845 -0.259
ENSG00000130821 E016 755.261378 0.0021753146 7.978425e-06 5.419854e-05 X 153691508 153691553 46 + 2.870 2.772 -0.326
ENSG00000130821 E017 1001.791068 0.0014414787 8.734278e-03 2.562142e-02 X 153691975 153692107 133 + 2.948 2.910 -0.127
ENSG00000130821 E018 49.954081 0.0057899600 8.302656e-14 2.389719e-12 X 153692108 153692230 123 + 1.922 1.475 -1.516
ENSG00000130821 E019 153.930805 0.0053029103 3.380508e-16 1.359718e-14 X 153692231 153693040 810 + 2.353 1.997 -1.193
ENSG00000130821 E020 437.382252 0.0007846394 9.143018e-01 9.497350e-01 X 153693041 153693053 13 + 2.544 2.563 0.063
ENSG00000130821 E021 933.705995 0.0006876616 1.865610e-01 3.094297e-01 X 153693054 153693175 122 + 2.854 2.896 0.139
ENSG00000130821 E022 428.165050 0.0014024274 1.374081e-01 2.444737e-01 X 153693263 153693266 4 + 2.503 2.561 0.195
ENSG00000130821 E023 726.982549 0.0001280687 3.206485e-01 4.638048e-01 X 153693267 153693326 60 + 2.751 2.782 0.104
ENSG00000130821 E024 691.156329 0.0001091016 4.085762e-01 5.521921e-01 X 153693327 153693366 40 + 2.732 2.761 0.095
ENSG00000130821 E025 644.804970 0.0001094940 7.795534e-01 8.582690e-01 X 153693462 153693491 30 + 2.717 2.728 0.034
ENSG00000130821 E026 910.961710 0.0000839955 2.248858e-01 3.562942e-01 X 153693492 153693586 95 + 2.849 2.881 0.108
ENSG00000130821 E027 931.735573 0.0007299505 9.262807e-04 3.707060e-03 X 153693905 153694017 113 + 2.821 2.903 0.270
ENSG00000130821 E028 5.919849 0.0030668258 1.888314e-01 3.122886e-01 X 153694018 153694129 112 + 0.884 0.717 -0.661
ENSG00000130821 E029 463.022102 0.0010008457 2.223316e-01 3.531844e-01 X 153694130 153694136 7 + 2.544 2.592 0.160
ENSG00000130821 E030 764.114617 0.0001333535 6.307931e-02 1.318381e-01 X 153694137 153694203 67 + 2.762 2.807 0.150
ENSG00000130821 E031 745.535150 0.0000897295 9.272432e-02 1.793784e-01 X 153694204 153694267 64 + 2.755 2.796 0.138
ENSG00000130821 E032 17.470727 0.0010447451 8.633018e-09 1.076879e-07 X 153694268 153694343 76 + 1.498 1.039 -1.618
ENSG00000130821 E033 1116.815296 0.0005585516 2.852674e-01 4.255727e-01 X 153694344 153694446 103 + 2.936 2.972 0.120
ENSG00000130821 E034 11.826640 0.0071055194 7.949290e-04 3.241641e-03 X 153694447 153694532 86 + 1.260 0.919 -1.236
ENSG00000130821 E035 1182.460841 0.0005344372 6.331997e-02 1.322330e-01 X 153694533 153694633 101 + 2.952 3.000 0.160
ENSG00000130821 E036 1377.460711 0.0003660657 4.276415e-07 3.834446e-06 X 153694719 153694889 171 + 2.984 3.074 0.301
ENSG00000130821 E037 4087.995780 0.0045801243 8.819489e-09 1.098288e-07 X 153695074 153697286 2213 + 3.378 3.566 0.625