ENSG00000130818

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253115 ENSG00000130818 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF426 protein_coding protein_coding 2.253601 0.7022589 3.395904 0.03184665 0.1190723 2.257564 1.48929850 0.63069754 2.0801122 0.06559005 0.05304396 1.705865 0.74097917 0.893333333 0.61313333 -0.28020000 0.0008634888 0.0008634888 FALSE TRUE
ENST00000589289 ENSG00000130818 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF426 protein_coding protein_coding 2.253601 0.7022589 3.395904 0.03184665 0.1190723 2.257564 0.08744192 0.00000000 0.2675442 0.00000000 0.21916370 4.794646 0.02465417 0.000000000 0.07523333 0.07523333 0.6601832324 0.0008634888 FALSE TRUE
ENST00000593003 ENSG00000130818 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF426 protein_coding protein_coding 2.253601 0.7022589 3.395904 0.03184665 0.1190723 2.257564 0.36180022 0.03364807 0.2164026 0.01847196 0.21640258 2.374901 0.12417500 0.048166667 0.06420000 0.01603333 0.7260555574 0.0008634888 FALSE TRUE
ENST00000635810 ENSG00000130818 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF426 protein_coding nonsense_mediated_decay 2.253601 0.7022589 3.395904 0.03184665 0.1190723 2.257564 0.01061708 0.03330662 0.0000000 0.03330662 0.00000000 -2.114588 0.00842500 0.052133333 0.00000000 -0.05213333 0.3953267499 0.0008634888   FALSE
MSTRG.16370.2 ENSG00000130818 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF426 protein_coding   2.253601 0.7022589 3.395904 0.03184665 0.1190723 2.257564 0.30146297 0.00460670 0.8318446 0.00460670 0.15739555 5.848852 0.10104583 0.006333333 0.24746667 0.24113333 0.0027592833 0.0008634888 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130818 E001 165.799601 0.012371100 1.545768e-11 3.120258e-10 19 9523223 9528006 4784 - 2.032 2.368 1.124
ENSG00000130818 E002 57.286799 0.006787847 8.521618e-03 2.509123e-02 19 9528007 9529296 1290 - 1.671 1.612 -0.198
ENSG00000130818 E003 25.440992 0.051097139 8.316934e-02 1.645297e-01 19 9529297 9529617 321 - 1.345 1.217 -0.449
ENSG00000130818 E004 8.447952 0.002148447 5.102175e-02 1.109864e-01 19 9529618 9529636 19 - 0.913 0.734 -0.703
ENSG00000130818 E005 14.593701 0.002885749 4.220054e-02 9.509426e-02 19 9530985 9531067 83 - 1.117 0.993 -0.451
ENSG00000130818 E006 16.314383 0.001159364 3.711559e-03 1.233066e-02 19 9532845 9532925 81 - 1.175 0.959 -0.784
ENSG00000130818 E007 14.373881 0.001199919 8.819487e-03 2.583138e-02 19 9533840 9533966 127 - 1.121 0.922 -0.732
ENSG00000130818 E008 9.200814 0.003059308 6.091076e-05 3.359717e-04 19 9535188 9535279 92 - 0.984 0.393 -2.552
ENSG00000130818 E009 10.439398 0.001681948 4.377479e-04 1.919676e-03 19 9536208 9536356 149 - 1.021 0.596 -1.689
ENSG00000130818 E010 0.000000       19 9536357 9536569 213 -      
ENSG00000130818 E011 6.682364 0.006513739 2.132878e-03 7.636159e-03 19 9538259 9538345 87 - 0.857 0.393 -2.072
ENSG00000130818 E012 4.858327 0.003884181 9.042346e-02 1.757909e-01 19 9538346 9538574 229 - 0.714 0.506 -0.917
ENSG00000130818 E013 4.720553 0.028129532 8.752119e-01 9.238405e-01 19 9538575 9538645 71 - 0.663 0.732 0.289