ENSG00000130751

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000439365 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding protein_coding 19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 2.7694960 6.5899638 1.1180796 0.8523150 0.03616115 -2.5485893 0.118416667 0.168800000 0.09070000 -0.078100000 7.064525e-02 4.734167e-26 FALSE TRUE
ENST00000594257 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding nonsense_mediated_decay 19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 0.7205332 2.4799534 0.0000000 0.2829369 0.00000000 -7.9599750 0.040512500 0.063566667 0.00000000 -0.063566667 1.128156e-12 4.734167e-26 FALSE FALSE
ENST00000594670 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding protein_coding 19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 0.4893856 0.1835402 1.1021511 0.1835402 0.39748355 2.5226474 0.036300000 0.005033333 0.08910000 0.084066667 3.788390e-02 4.734167e-26 FALSE FALSE
ENST00000601169 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding retained_intron 19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 1.7390526 3.1386261 1.3341850 0.2657639 0.31649296 -1.2279908 0.103025000 0.079566667 0.10763333 0.028066667 6.503923e-01 4.734167e-26 FALSE TRUE
ENST00000602189 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding protein_coding 19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 1.3113528 2.6170970 0.9393648 0.4168355 0.24075001 -1.4684351 0.065658333 0.066033333 0.07576667 0.009733333 8.917368e-01 4.734167e-26 FALSE TRUE
ENST00000602212 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding protein_coding 19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 2.4128715 2.9815071 2.4796617 1.1400377 0.66031685 -0.2649227 0.137379167 0.074633333 0.20100000 0.126366667 1.636324e-01 4.734167e-26 FALSE TRUE
MSTRG.17389.1 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding   19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 0.3446609 0.0000000 1.0304676 0.0000000 0.59608237 6.7010882 0.023695833 0.000000000 0.08233333 0.082333333 9.135300e-02 4.734167e-26 FALSE TRUE
MSTRG.17389.10 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding   19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 0.2829885 1.9855776 0.0000000 1.0135921 0.00000000 -7.6406625 0.008441667 0.051866667 0.00000000 -0.051866667 2.782366e-01 4.734167e-26 FALSE TRUE
MSTRG.17389.18 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding   19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 2.1698846 6.7589404 0.0000000 0.4218908 0.00000000 -9.4027862 0.103012500 0.172533333 0.00000000 -0.172533333 4.734167e-26 4.734167e-26 FALSE TRUE
MSTRG.17389.9 ENSG00000130751 HEK293_OSMI2_2hA HEK293_TMG_2hB NPAS1 protein_coding   19.51807 39.47963 12.35059 1.498064 0.2511493 -1.675726 1.1224008 1.1739378 0.3995967 1.1739378 0.21128009 -1.5313173 0.050141667 0.028566667 0.03303333 0.004466667 5.505601e-01 4.734167e-26 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130751 E001 0.8105550 0.0153787590 8.192273e-03 2.427164e-02 19 47019801 47019806 6 + 0.531 0.071 -3.762
ENSG00000130751 E002 2.6811238 0.0065830883 5.403635e-06 3.817079e-05 19 47019807 47019836 30 + 0.944 0.232 -3.463
ENSG00000130751 E003 4.2591821 0.0045467082 2.734530e-06 2.065705e-05 19 47019837 47019838 2 + 1.072 0.413 -2.763
ENSG00000130751 E004 5.9533451 0.0037283706 1.313830e-10 2.274317e-09 19 47019839 47019844 6 + 1.250 0.442 -3.248
ENSG00000130751 E005 6.5016673 0.0071786984 7.901464e-10 1.194755e-08 19 47019845 47019845 1 + 1.264 0.494 -3.034
ENSG00000130751 E006 7.3810193 0.0163882700 1.319827e-08 1.590872e-07 19 47019846 47019847 2 + 1.292 0.564 -2.803
ENSG00000130751 E007 8.1645190 0.0027007641 1.808395e-11 3.607022e-10 19 47019848 47019848 1 + 1.342 0.583 -2.892
ENSG00000130751 E008 18.7262885 0.1069742684 1.943990e-04 9.399772e-04 19 47019849 47019869 21 + 1.601 0.989 -2.153
ENSG00000130751 E009 23.7847696 0.0770400965 1.165219e-04 5.979554e-04 19 47019870 47019898 29 + 1.668 1.116 -1.919
ENSG00000130751 E010 67.6127050 0.0237504078 2.821543e-03 9.738434e-03 19 47019899 47019997 99 + 1.926 1.670 -0.866
ENSG00000130751 E011 0.3634088 0.3691449291 1.000000e+00   19 47020886 47021005 120 + 0.001 0.132 7.604
ENSG00000130751 E012 105.2696668 0.0133808529 1.182636e-01 2.174432e-01 19 47021006 47021169 164 + 2.008 1.899 -0.365
ENSG00000130751 E013 95.3269152 0.0072010718 3.800894e-01 5.243569e-01 19 47021612 47021847 236 + 1.926 1.866 -0.202
ENSG00000130751 E014 64.8375309 0.0088247173 4.720771e-02 1.042041e-01 19 47032278 47032351 74 + 1.826 1.685 -0.476
ENSG00000130751 E015 115.9117659 0.0033730528 9.479434e-01 9.712812e-01 19 47032643 47032732 90 + 1.970 1.962 -0.028
ENSG00000130751 E016 9.5168076 0.0162344319 5.087846e-03 1.616999e-02 19 47035329 47035331 3 + 1.187 0.835 -1.300
ENSG00000130751 E017 15.4354487 0.0170653908 2.177185e-02 5.520163e-02 19 47035332 47035347 16 + 1.318 1.058 -0.923
ENSG00000130751 E018 15.5097335 0.0120294394 1.014184e-02 2.911298e-02 19 47035348 47035349 2 + 1.330 1.052 -0.989
ENSG00000130751 E019 17.2059067 0.0162726788 2.268552e-02 5.710399e-02 19 47035350 47035358 9 + 1.354 1.103 -0.885
ENSG00000130751 E020 14.1123958 0.0012847018 2.462110e-01 3.815080e-01 19 47035860 47035888 29 + 1.187 1.058 -0.466
ENSG00000130751 E021 17.1600317 0.0010525105 1.304348e-01 2.347639e-01 19 47035889 47035963 75 + 1.279 1.126 -0.541
ENSG00000130751 E022 231.0236992 0.0006857550 1.910027e-01 3.150103e-01 19 47035964 47036129 166 + 2.238 2.264 0.086
ENSG00000130751 E023 103.3141489 0.0042873269 1.405359e-01 2.487745e-01 19 47039036 47039076 41 + 1.863 1.929 0.223
ENSG00000130751 E024 155.2259099 0.0044499867 2.249286e-02 5.670737e-02 19 47039077 47039151 75 + 2.015 2.110 0.318
ENSG00000130751 E025 9.7681314 0.0018113215 1.831361e-01 3.051317e-01 19 47039404 47039406 3 + 0.806 0.969 0.621
ENSG00000130751 E026 214.1588512 0.0055351001 3.771917e-02 8.680602e-02 19 47039407 47039564 158 + 2.168 2.248 0.267
ENSG00000130751 E027 246.7843783 0.0028553851 6.370541e-02 1.328738e-01 19 47040444 47040550 107 + 2.250 2.304 0.182
ENSG00000130751 E028 18.8174980 0.0020610123 6.176299e-02 1.295900e-01 19 47040551 47040758 208 + 1.342 1.164 -0.627
ENSG00000130751 E029 85.4593237 0.0005993384 8.703184e-02 1.706453e-01 19 47040978 47041024 47 + 1.781 1.849 0.229
ENSG00000130751 E030 180.2847141 0.0003519357 4.480911e-03 1.448726e-02 19 47041025 47041125 101 + 2.089 2.169 0.268
ENSG00000130751 E031 219.8311969 0.0012237371 1.144450e-03 4.452414e-03 19 47042810 47042904 95 + 2.165 2.260 0.317
ENSG00000130751 E032 20.8198055 0.0240041126 1.129065e-01 2.097815e-01 19 47045135 47045190 56 + 1.072 1.264 0.686
ENSG00000130751 E033 384.1711177 0.0009947411 1.314380e-04 6.651670e-04 19 47045191 47045775 585 + 2.415 2.498 0.278