ENSG00000130741

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253039 ENSG00000130741 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2S3 protein_coding protein_coding 139.5567 88.37674 211.1177 9.455831 8.673452 1.256214 94.87378 78.192820 134.76898 10.9581764 6.3666951 0.785303 0.7136083 0.8790333 0.63803333 -0.24100000 0.0002202077 8.72373e-05 FALSE TRUE
ENST00000460032 ENSG00000130741 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2S3 protein_coding processed_transcript 139.5567 88.37674 211.1177 9.455831 8.673452 1.256214 12.29289 3.527620 17.69277 0.7363701 0.5149735 2.323124 0.0954875 0.0420000 0.08406667 0.04206667 0.0926230221 8.72373e-05   FALSE
MSTRG.34026.4 ENSG00000130741 HEK293_OSMI2_2hA HEK293_TMG_2hB EIF2S3 protein_coding   139.5567 88.37674 211.1177 9.455831 8.673452 1.256214 19.27326 1.792644 32.82884 1.0136225 1.9892371 4.187217 0.1066708 0.0226000 0.15626667 0.13366667 0.2437705922 8.72373e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130741 E001 7.578788 3.410367e-02 6.615267e-02 1.369679e-01 X 24054679 24054680 2 + 0.995 0.758 -0.910
ENSG00000130741 E002 13.658869 1.325328e-03 4.096963e-02 9.282531e-02 X 24054681 24054707 27 + 1.201 1.042 -0.572
ENSG00000130741 E003 24.986777 1.165461e-02 3.385686e-03 1.139592e-02 X 24054708 24054739 32 + 1.469 1.235 -0.813
ENSG00000130741 E004 33.457303 3.821827e-03 4.158261e-05 2.386957e-04 X 24054740 24054759 20 + 1.596 1.333 -0.906
ENSG00000130741 E005 106.891483 2.977154e-04 3.892832e-12 8.669160e-11 X 24054760 24054945 186 + 2.084 1.860 -0.752
ENSG00000130741 E006 61.751791 3.700560e-04 1.060288e-07 1.072041e-06 X 24054946 24054955 10 + 1.850 1.628 -0.750
ENSG00000130741 E007 121.961778 2.456129e-04 2.039509e-27 2.750027e-25 X 24054956 24054970 15 + 2.174 1.806 -1.236
ENSG00000130741 E008 701.438579 6.360476e-03 5.237684e-08 5.624120e-07 X 24054971 24055037 67 + 2.890 2.694 -0.651
ENSG00000130741 E009 887.159819 6.121858e-03 7.703256e-08 7.996486e-07 X 24055615 24055678 64 + 2.989 2.804 -0.615
ENSG00000130741 E010 1200.748979 2.191318e-03 2.432314e-12 5.602366e-11 X 24057421 24057548 128 + 3.109 2.967 -0.469
ENSG00000130741 E011 474.324584 7.802637e-04 1.039416e-12 2.544770e-11 X 24057633 24057641 9 + 2.700 2.583 -0.392
ENSG00000130741 E012 1118.123800 2.130032e-03 1.665014e-09 2.367752e-08 X 24057642 24057754 113 + 3.070 2.955 -0.385
ENSG00000130741 E013 924.612766 1.537468e-03 8.598726e-10 1.289584e-08 X 24060088 24060182 95 + 2.984 2.881 -0.342
ENSG00000130741 E014 1.068433 1.336471e-02 7.573520e-01 8.422177e-01 X 24060183 24060479 297 + 0.324 0.291 -0.221
ENSG00000130741 E015 0.808377 3.689227e-02 2.525777e-01 3.889553e-01 X 24061890 24061930 41 + 0.160 0.385 1.685
ENSG00000130741 E016 2.064948 7.243862e-03 1.760732e-02 4.628739e-02 X 24061931 24062009 79 + 0.590 0.169 -2.598
ENSG00000130741 E017 1118.329667 3.256792e-03 1.601282e-04 7.923681e-04 X 24062416 24062574 159 + 3.059 2.979 -0.264
ENSG00000130741 E018 2.302224 3.156949e-02 9.476494e-01 9.710692e-01 X 24062575 24062626 52 + 0.508 0.528 0.094
ENSG00000130741 E019 831.491801 2.629872e-03 5.238566e-02 1.134103e-01 X 24064201 24064335 135 + 2.911 2.890 -0.071
ENSG00000130741 E020 673.053845 2.054602e-03 3.843287e-01 5.284927e-01 X 24065998 24066092 95 + 2.807 2.820 0.040
ENSG00000130741 E021 1235.263666 9.334604e-04 9.968394e-01 1.000000e+00 X 24067964 24068108 145 + 3.062 3.099 0.122
ENSG00000130741 E022 1373.917012 7.621177e-04 7.891757e-01 8.650422e-01 X 24071558 24071712 155 + 3.107 3.149 0.140
ENSG00000130741 E023 590.006700 1.010105e-04 9.436529e-01 9.686517e-01 X 24071713 24071727 15 + 2.742 2.785 0.144
ENSG00000130741 E024 1105.606493 7.106289e-05 5.071042e-01 6.427206e-01 X 24073091 24073181 91 + 3.011 3.063 0.172
ENSG00000130741 E025 1078.575158 7.734874e-05 5.571214e-01 6.856019e-01 X 24073182 24073261 80 + 3.001 3.052 0.169
ENSG00000130741 E026 639.613643 1.210411e-04 5.381447e-01 6.696082e-01 X 24073262 24073263 2 + 2.773 2.827 0.178
ENSG00000130741 E027 73.879264 8.412647e-03 3.290170e-06 2.441874e-05 X 24073264 24073411 148 + 1.934 1.667 -0.901
ENSG00000130741 E028 26.751822 9.406431e-04 8.716997e-09 1.086596e-07 X 24073412 24073528 117 + 1.533 1.111 -1.473
ENSG00000130741 E029 2834.099893 1.411812e-03 1.945174e-13 5.309141e-12 X 24076722 24077343 622 + 3.368 3.536 0.560
ENSG00000130741 E030 3661.598521 7.710148e-03 5.571049e-08 5.940999e-07 X 24077344 24078810 1467 + 3.441 3.687 0.815