ENSG00000130731

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000301686 ENSG00000130731 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL26 protein_coding protein_coding 258.1548 516.1154 127.3746 36.08351 2.8838 -2.01853 29.07415 50.45582 29.20970 1.778173 0.6712191 -0.7883651 0.13270000 0.09853333 0.22980000 0.1312667 1.850709e-12 2.210774e-18 FALSE TRUE
ENST00000338401 ENSG00000130731 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL26 protein_coding protein_coding 258.1548 516.1154 127.3746 36.08351 2.8838 -2.01853 95.59010 206.95854 30.70604 14.989698 0.9349477 -2.7523475 0.32567083 0.40100000 0.24120000 -0.1598000 2.210774e-18 2.210774e-18 FALSE TRUE
ENST00000397664 ENSG00000130731 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL26 protein_coding protein_coding 258.1548 516.1154 127.3746 36.08351 2.8838 -2.01853 42.25733 88.72372 14.69392 8.214974 0.3487084 -2.5932815 0.15642083 0.17146667 0.11536667 -0.0561000 1.133769e-06 2.210774e-18 FALSE TRUE
ENST00000448973 ENSG00000130731 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL26 protein_coding retained_intron 258.1548 516.1154 127.3746 36.08351 2.8838 -2.01853 17.24544 21.79175 11.70223 2.142587 0.4454147 -0.8964285 0.09510417 0.04226667 0.09196667 0.0497000 3.225235e-08 2.210774e-18 TRUE FALSE
ENST00000568077 ENSG00000130731 HEK293_OSMI2_2hA HEK293_TMG_2hB METTL26 protein_coding nonsense_mediated_decay 258.1548 516.1154 127.3746 36.08351 2.8838 -2.01853 36.75288 77.44462 18.13960 4.938041 0.6200380 -2.0934133 0.14546250 0.15026667 0.14236667 -0.0079000 6.561355e-01 2.210774e-18 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130731 E001 3.061075 2.765073e-02 1.099159e-01 2.054201e-01 16 634427 634428 2 - 0.223 0.563 1.983
ENSG00000130731 E002 28.295912 7.142466e-04 1.086813e-02 3.085116e-02 16 634429 634429 1 - 1.139 1.371 0.817
ENSG00000130731 E003 202.710467 2.759087e-04 1.178088e-08 1.433708e-07 16 634430 634435 6 - 2.002 2.204 0.674
ENSG00000130731 E004 223.040265 2.047581e-04 6.005474e-09 7.731170e-08 16 634436 634437 2 - 2.049 2.243 0.649
ENSG00000130731 E005 751.806229 9.520707e-04 3.415942e-05 2.003343e-04 16 634438 634501 64 - 2.669 2.757 0.295
ENSG00000130731 E006 1086.928328 1.096301e-03 4.539677e-08 4.938433e-07 16 634502 634512 11 - 2.808 2.920 0.372
ENSG00000130731 E007 1043.951043 1.487117e-03 1.677977e-05 1.058814e-04 16 634513 634525 13 - 2.805 2.901 0.320
ENSG00000130731 E008 2144.616634 7.984248e-04 5.573362e-07 4.887321e-06 16 634526 634625 100 - 3.135 3.210 0.248
ENSG00000130731 E009 1744.913903 4.038866e-04 5.400793e-06 3.815356e-05 16 634626 634644 19 - 3.059 3.118 0.196
ENSG00000130731 E010 30.449806 6.691296e-04 8.709670e-01 9.210403e-01 16 634664 634718 55 - 1.365 1.371 0.020
ENSG00000130731 E011 1548.547866 1.951954e-04 1.195707e-06 9.767299e-06 16 634719 634736 18 - 3.008 3.065 0.192
ENSG00000130731 E012 2127.514231 5.379656e-05 7.490732e-09 9.448676e-08 16 634737 634797 61 - 3.149 3.201 0.172
ENSG00000130731 E013 945.136109 1.037750e-04 3.274056e-13 8.649562e-12 16 634889 634948 60 - 2.934 2.821 -0.377
ENSG00000130731 E014 607.217077 1.146222e-04 1.568273e-08 1.863777e-07 16 634949 634956 8 - 2.739 2.629 -0.365
ENSG00000130731 E015 404.693558 3.863623e-03 3.222562e-05 1.902061e-04 16 634957 635063 107 - 2.601 2.445 -0.519
ENSG00000130731 E016 318.263381 1.929150e-03 2.565894e-13 6.884307e-12 16 635064 635280 217 - 2.555 2.323 -0.775
ENSG00000130731 E017 1064.284703 3.972629e-04 4.914974e-06 3.504035e-05 16 635281 635340 60 - 2.959 2.878 -0.266
ENSG00000130731 E018 81.550825 2.664008e-03 2.078990e-09 2.900752e-08 16 635341 635517 177 - 2.022 1.715 -1.035
ENSG00000130731 E019 96.566284 5.610948e-04 9.797191e-08 9.965896e-07 16 635518 635611 94 - 2.041 1.804 -0.796
ENSG00000130731 E020 744.568317 2.362008e-03 8.488262e-25 9.121247e-23 16 635612 635774 163 - 2.961 2.679 -0.940
ENSG00000130731 E021 93.331220 5.259482e-03 3.940607e-20 2.546167e-18 16 635775 636093 319 - 2.196 1.714 -1.622
ENSG00000130731 E022 2023.592300 9.141012e-03 4.533636e-01 5.940923e-01 16 636094 636366 273 - 3.200 3.168 -0.107