ENSG00000130725

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000253023 ENSG00000130725 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2M protein_coding protein_coding 320.8433 561.1594 213.3276 19.6897 3.584969 -1.395298 195.47836 378.26901 127.273241 7.833964 1.346470 -1.5714082 0.5768792 0.67490000 0.5969333 -0.07796667 0.02056292 1.345862e-29 FALSE  
ENST00000595957 ENSG00000130725 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2M protein_coding protein_coding 320.8433 561.1594 213.3276 19.6897 3.584969 -1.395298 37.05726 30.79267 40.818917 12.539259 2.679819 0.4065359 0.1425083 0.05356667 0.1912000 0.13763333 0.06551452 1.345862e-29    
MSTRG.17868.1 ENSG00000130725 HEK293_OSMI2_2hA HEK293_TMG_2hB UBE2M protein_coding   320.8433 561.1594 213.3276 19.6897 3.584969 -1.395298 40.39122 96.01390 6.995656 4.657072 3.618769 -3.7768015 0.1079250 0.17146667 0.0331000 -0.13836667 0.39796001 1.345862e-29 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130725 E001 4380.509320 9.974185e-04 2.384480e-27 3.194715e-25 19 58555712 58556140 429 - 3.407 3.570 0.543
ENSG00000130725 E002 3390.532009 5.747737e-05 1.396670e-05 8.972665e-05 19 58556141 58556200 60 - 3.406 3.436 0.099
ENSG00000130725 E003 3366.394033 1.862516e-04 1.508764e-01 2.628642e-01 19 58556201 58556229 29 - 3.419 3.429 0.034
ENSG00000130725 E004 84.920178 5.670800e-03 5.450029e-02 1.171210e-01 19 58556230 58556315 86 - 1.924 1.813 -0.373
ENSG00000130725 E005 3862.464995 1.840366e-04 2.905765e-03 9.986630e-03 19 58556316 58556379 64 - 3.468 3.491 0.077
ENSG00000130725 E006 81.497301 3.521270e-04 3.796060e-05 2.200386e-04 19 58556380 58556490 111 - 1.962 1.776 -0.626
ENSG00000130725 E007 30.043837 6.509346e-04 9.738241e-09 1.203523e-07 19 58556582 58556593 12 - 1.671 1.285 -1.329
ENSG00000130725 E008 52.085333 5.666506e-04 9.920590e-13 2.437963e-11 19 58556594 58556686 93 - 1.895 1.520 -1.271
ENSG00000130725 E009 4427.042529 2.854176e-04 6.214176e-01 7.383885e-01 19 58556687 58556790 104 - 3.552 3.545 -0.024
ENSG00000130725 E010 56.457345 3.149500e-03 1.332129e-01 2.386005e-01 19 58556791 58556794 4 - 1.738 1.644 -0.318
ENSG00000130725 E011 2590.067770 1.254878e-04 1.157284e-01 2.138549e-01 19 58556892 58556930 39 - 3.328 3.309 -0.062
ENSG00000130725 E012 21.447031 1.155528e-03 4.923516e-06 3.508936e-05 19 58556931 58557062 132 - 1.521 1.161 -1.256
ENSG00000130725 E013 2964.329686 7.858255e-05 4.243839e-02 9.551549e-02 19 58557063 58557157 95 - 3.388 3.367 -0.070
ENSG00000130725 E014 5.825028 2.772855e-03 5.182791e-02 1.124160e-01 19 58557968 58558272 305 - 0.942 0.675 -1.056
ENSG00000130725 E015 1642.823134 2.860408e-03 6.778334e-03 2.064592e-02 19 58558273 58558354 82 - 3.176 3.100 -0.252
ENSG00000130725 E016 967.120709 5.089399e-03 1.291962e-09 1.875975e-08 19 58558355 58558519 165 - 3.055 2.832 -0.739
ENSG00000130725 E017 435.454626 2.371892e-02 1.289023e-07 1.281830e-06 19 58558520 58558658 139 - 2.830 2.425 -1.349
ENSG00000130725 E018 296.414868 2.760010e-03 8.900423e-05 4.703371e-04 19 58558659 58558693 35 - 2.480 2.341 -0.464
ENSG00000130725 E019 654.176771 5.853928e-03 1.611390e-02 4.298771e-02 19 58558694 58558954 261 - 2.794 2.695 -0.329