ENSG00000130684

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000376436 ENSG00000130684 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF337 protein_coding protein_coding 11.89698 8.826077 12.38093 0.5317609 0.6531289 0.4878064 7.856302 6.7768359 6.822507 0.5101761 0.5908443 0.009675821 0.6628667 0.77043333 0.54906667 -0.22136667 0.03126403 0.03126403 FALSE TRUE
MSTRG.20518.3 ENSG00000130684 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF337 protein_coding   11.89698 8.826077 12.38093 0.5317609 0.6531289 0.4878064 1.050109 0.6258247 1.107165 0.1824753 0.1638319 0.813141581 0.0937375 0.07266667 0.09133333 0.01866667 0.78932795 0.03126403 FALSE TRUE
MSTRG.20518.5 ENSG00000130684 HEK293_OSMI2_2hA HEK293_TMG_2hB ZNF337 protein_coding   11.89698 8.826077 12.38093 0.5317609 0.6531289 0.4878064 2.353509 1.2013612 3.586733 0.6118314 0.1433371 1.570057909 0.1907542 0.13046667 0.29066667 0.16020000 0.56599854 0.03126403   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130684 E001 1.2501240 0.0438435786 5.165853e-01 6.511346e-01 20 25673195 25673874 680 - 0.411 0.302 -0.652
ENSG00000130684 E002 0.2214452 0.0401911909 4.722861e-01   20 25673875 25673896 22 - 0.000 0.126 10.164
ENSG00000130684 E003 0.9662351 0.0140858455 9.793901e-01 9.911626e-01 20 25673897 25674214 318 - 0.289 0.302 0.086
ENSG00000130684 E004 480.0995954 0.0017685712 1.051120e-10 1.847917e-09 20 25674215 25676066 1852 - 2.612 2.737 0.415
ENSG00000130684 E005 120.2607792 0.0002540079 6.176330e-01 7.354002e-01 20 25676067 25676318 252 - 2.068 2.099 0.106
ENSG00000130684 E006 82.9963324 0.0004407517 7.415731e-01 8.306950e-01 20 25676319 25676486 168 - 1.924 1.928 0.013
ENSG00000130684 E007 113.6407805 0.0003566718 4.000621e-04 1.774622e-03 20 25676487 25676901 415 - 2.109 2.013 -0.323
ENSG00000130684 E008 32.7871122 0.0152170571 2.175662e-02 5.516969e-02 20 25676902 25676948 47 - 1.613 1.435 -0.612
ENSG00000130684 E009 31.7622819 0.0024481694 1.614989e-02 4.305766e-02 20 25676949 25676995 47 - 1.583 1.445 -0.473
ENSG00000130684 E010 31.2883154 0.0006866181 1.561711e-01 2.700353e-01 20 25676996 25677037 42 - 1.545 1.475 -0.242
ENSG00000130684 E011 41.4489688 0.0006808817 9.488864e-01 9.719463e-01 20 25685567 25685662 96 - 1.617 1.629 0.042
ENSG00000130684 E012 44.6699555 0.0058507780 6.845948e-02 1.408191e-01 20 25685996 25686122 127 - 1.704 1.603 -0.342
ENSG00000130684 E013 0.2944980 0.3331337207 2.894182e-01   20 25686123 25686249 127 - 0.212 0.000 -10.905
ENSG00000130684 E014 38.2358283 0.0194167585 3.677613e-03 1.223183e-02 20 25686391 25686466 76 - 1.699 1.461 -0.811
ENSG00000130684 E015 36.8604357 0.0006025120 8.662961e-01 9.179071e-01 20 25686467 25686957 491 - 1.564 1.590 0.087
ENSG00000130684 E016 5.1537805 0.0093602311 2.156777e-04 1.031215e-03 20 25696459 25696758 300 - 0.993 0.479 -2.138
ENSG00000130684 E017 21.6470778 0.0217389574 8.695492e-04 3.507580e-03 20 25696759 25696863 105 - 1.491 1.165 -1.140