ENSG00000130669

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000358301 ENSG00000130669 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK4 protein_coding protein_coding 28.87962 43.87289 20.43546 6.103412 0.522642 -1.101878 2.384894 3.875082 1.671672 0.2851258 0.1350250 -1.2080488 0.07822917 0.09040000 0.08223333 -0.008166667 0.84395541 0.02092542 FALSE  
ENST00000360442 ENSG00000130669 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK4 protein_coding protein_coding 28.87962 43.87289 20.43546 6.103412 0.522642 -1.101878 10.314147 16.456630 6.596862 0.5190435 0.1580790 -1.3175082 0.36006667 0.38943333 0.32293333 -0.066500000 0.60717217 0.02092542 FALSE  
ENST00000593480 ENSG00000130669 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK4 protein_coding protein_coding 28.87962 43.87289 20.43546 6.103412 0.522642 -1.101878 8.211711 10.407111 7.004601 2.9469806 0.3867449 -0.5705223 0.29350417 0.22586667 0.34256667 0.116700000 0.24754540 0.02092542 FALSE  
ENST00000602004 ENSG00000130669 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK4 protein_coding protein_coding 28.87962 43.87289 20.43546 6.103412 0.522642 -1.101878 3.653306 4.280071 2.924379 2.2621776 0.3738013 -0.5479466 0.12505833 0.08543333 0.14296667 0.057533333 0.73876541 0.02092542 FALSE  
MSTRG.17114.1 ENSG00000130669 HEK293_OSMI2_2hA HEK293_TMG_2hB PAK4 protein_coding   28.87962 43.87289 20.43546 6.103412 0.522642 -1.101878 3.818587 7.679550 1.872228 1.2439798 0.1841725 -2.0304580 0.12605000 0.17783333 0.09153333 -0.086300000 0.02092542 0.02092542 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130669 E001 0.1482932 0.0415788381 1.000000e+00   19 39125721 39125734 14 + 0.000 0.077 9.055
ENSG00000130669 E002 3.8246275 0.0043836087 4.079706e-01 0.5515586867 19 39125735 39125769 35 + 0.502 0.654 0.689
ENSG00000130669 E003 5.3755156 0.0077221765 6.291158e-01 0.7445515888 19 39125770 39125781 12 + 0.682 0.768 0.353
ENSG00000130669 E004 8.6624910 0.0019531155 3.496152e-01 0.4938285476 19 39125782 39125785 4 + 0.809 0.944 0.519
ENSG00000130669 E005 39.8505223 0.0042687351 8.782593e-01 0.9258239941 19 39125786 39125797 12 + 1.512 1.539 0.093
ENSG00000130669 E006 40.1053787 0.0053643446 8.538448e-01 0.9095799698 19 39125798 39125798 1 + 1.512 1.542 0.103
ENSG00000130669 E007 51.2480160 0.0016588782 9.956956e-02 0.1898236366 19 39125799 39125801 3 + 1.698 1.619 -0.269
ENSG00000130669 E008 91.5193330 0.0064234027 8.810489e-02 0.1722429842 19 39125802 39125812 11 + 1.944 1.867 -0.259
ENSG00000130669 E009 94.5900484 0.0042033478 7.465314e-02 0.1509835649 19 39125813 39125813 1 + 1.955 1.882 -0.246
ENSG00000130669 E010 170.1365250 0.0036079760 4.314828e-01 0.5740347222 19 39125814 39125833 20 + 2.165 2.150 -0.048
ENSG00000130669 E011 231.4066177 0.0024441932 4.507438e-01 0.5917921310 19 39125834 39125879 46 + 2.293 2.285 -0.027
ENSG00000130669 E012 244.9232964 0.0001887634 1.286177e-01 0.2322340978 19 39125880 39125919 40 + 2.330 2.305 -0.081
ENSG00000130669 E013 0.1817044 0.0392620772 1.000000e+00   19 39152120 39152392 273 + 0.000 0.077 9.050
ENSG00000130669 E014 0.8104533 0.0151159061 4.889003e-01 0.6264156600 19 39156702 39156850 149 + 0.320 0.200 -0.897
ENSG00000130669 E015 0.5451131 0.6893840415 7.313261e-01 0.8230171464 19 39167992 39168085 94 + 0.000 0.202 10.653
ENSG00000130669 E016 262.4321310 0.0001772679 1.942994e-02 0.0502675857 19 39168230 39168302 73 + 2.373 2.329 -0.144
ENSG00000130669 E017 66.0967970 0.0073894801 2.520171e-01 0.3883352654 19 39168303 39168312 10 + 1.784 1.731 -0.178
ENSG00000130669 E018 0.7696683 0.0162545189 1.103807e-01 0.2060936587 19 39168625 39168840 216 + 0.420 0.143 -2.067
ENSG00000130669 E019 540.8412849 0.0021502767 4.044079e-04 0.0017915541 19 39169532 39169757 226 + 2.713 2.637 -0.251
ENSG00000130669 E020 385.3113992 0.0008581766 7.726583e-03 0.0230926047 19 39172918 39173106 189 + 2.543 2.496 -0.157
ENSG00000130669 E021 487.8896835 0.0028550908 4.120795e-01 0.5556365378 19 39173107 39173376 270 + 2.574 2.621 0.156
ENSG00000130669 E022 715.0121190 0.0026050888 8.498495e-01 0.9068167972 19 39173576 39174010 435 + 2.758 2.783 0.083
ENSG00000130669 E023 226.4572766 0.0002444610 2.522315e-01 0.3885645361 19 39174931 39174935 5 + 2.290 2.274 -0.053
ENSG00000130669 E024 316.5507727 0.0010470327 2.977368e-01 0.4390853203 19 39174936 39174966 31 + 2.433 2.422 -0.038
ENSG00000130669 E025 462.2640946 0.0018717957 9.815773e-01 0.9925428796 19 39174967 39175064 98 + 2.576 2.593 0.058
ENSG00000130669 E026 513.0755908 0.0008633724 8.814227e-01 0.9280126572 19 39175312 39175438 127 + 2.622 2.636 0.048
ENSG00000130669 E027 1.1426264 0.0117724797 1.193511e-01 0.2190153708 19 39176489 39176589 101 + 0.000 0.336 11.636
ENSG00000130669 E028 486.9676569 0.0003992866 1.054627e-01 0.1988033009 19 39176590 39176715 126 + 2.622 2.606 -0.053
ENSG00000130669 E029 515.5947617 0.0005460013 3.286197e-01 0.4721192875 19 39177675 39177809 135 + 2.605 2.642 0.125
ENSG00000130669 E030 1597.9126849 0.0028940783 2.293683e-05 0.0001402298 19 39178424 39182816 4393 + 3.031 3.152 0.402