ENSG00000130584

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000245663 ENSG00000130584 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB46 protein_coding protein_coding 1.981377 2.501394 1.863241 0.2996277 0.183764 -0.4229515 1.03360088 1.67653500 1.4249426 0.83881029 0.05544252 -0.2330692 0.50792083 0.600033333 0.77860000 0.17856667 0.9638916816 0.0004363559 FALSE TRUE
ENST00000395104 ENSG00000130584 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB46 protein_coding protein_coding 1.981377 2.501394 1.863241 0.2996277 0.183764 -0.4229515 0.63719646 0.63122834 0.0000000 0.63122834 0.00000000 -6.0027663 0.33736250 0.329933333 0.00000000 -0.32993333 0.7705000622 0.0004363559 FALSE TRUE
ENST00000650966 ENSG00000130584 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB46 protein_coding nonsense_mediated_decay 1.981377 2.501394 1.863241 0.2996277 0.183764 -0.4229515 0.08778098 0.01721539 0.2281262 0.00936322 0.01877716 3.1292319 0.05140000 0.007366667 0.12656667 0.11920000 0.0004363559 0.0004363559 TRUE TRUE
MSTRG.21014.4 ENSG00000130584 HEK293_OSMI2_2hA HEK293_TMG_2hB ZBTB46 protein_coding   1.981377 2.501394 1.863241 0.2996277 0.183764 -0.4229515 0.07332615 0.00000000 0.2101726 0.00000000 0.21017258 4.4605629 0.03579583 0.000000000 0.09483333 0.09483333 0.7592091687 0.0004363559 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130584 E001 0.0000000       20 63743668 63743669 2 -      
ENSG00000130584 E002 30.0770606 0.0045538914 0.0746094652 0.150908463 20 63743670 63744524 855 - 1.379 1.506 0.437
ENSG00000130584 E003 15.0452197 0.0017471002 0.1902136003 0.314042162 20 63744525 63744616 92 - 1.096 1.216 0.427
ENSG00000130584 E004 22.6144381 0.0078483430 0.0746884588 0.151040825 20 63744617 63744681 65 - 1.235 1.388 0.536
ENSG00000130584 E005 54.4394117 0.0159340749 0.0690086306 0.141729893 20 63744682 63745142 461 - 1.617 1.754 0.465
ENSG00000130584 E006 27.7465728 0.0078262906 0.2622136993 0.399971003 20 63745143 63745311 169 - 1.364 1.452 0.304
ENSG00000130584 E007 90.6349302 0.0003943305 0.5614935653 0.689290662 20 63745312 63746871 1560 - 1.915 1.935 0.066
ENSG00000130584 E008 23.4059042 0.0009132905 0.6762317839 0.781259081 20 63746872 63747301 430 - 1.331 1.361 0.104
ENSG00000130584 E009 16.8143522 0.0011739182 0.0593471492 0.125522093 20 63752686 63752861 176 - 1.322 1.167 -0.545
ENSG00000130584 E010 3.2922805 0.0047237222 0.0064520921 0.019798602 20 63767269 63767982 714 - 0.842 0.438 -1.772
ENSG00000130584 E011 1.3254661 0.0119092203 0.1502986160 0.262091512 20 63768073 63768118 46 - 0.495 0.243 -1.507
ENSG00000130584 E012 1.4653428 0.0874477570 0.0021100756 0.007565674 20 63769250 63769383 134 - 0.683 0.096 -3.946
ENSG00000130584 E013 1.0234039 0.1424461443 0.0612089373 0.128662645 20 63775256 63775401 146 - 0.000 0.395 12.234
ENSG00000130584 E014 31.6264988 0.0053972913 0.1916198779 0.315785998 20 63775678 63775962 285 - 1.539 1.451 -0.301
ENSG00000130584 E015 0.0000000       20 63787720 63788309 590 -      
ENSG00000130584 E016 71.5715826 0.0074210943 0.0192362198 0.049846042 20 63789821 63790790 970 - 1.903 1.788 -0.388
ENSG00000130584 E017 0.8857808 0.9158302178 0.4929729275 0.630054423 20 63790791 63790923 133 - 0.000 0.328 10.781
ENSG00000130584 E018 3.4910265 0.0985397285 0.0009888601 0.003920405 20 63831097 63831264 168 - 0.938 0.303 -2.924
ENSG00000130584 E019 0.0000000       20 63831949 63832038 90 -