ENSG00000130520

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000252816 ENSG00000130520 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM4 protein_coding protein_coding 337.5977 611.5309 184.9882 33.47096 1.598952 -1.724938 186.90965 342.82186 87.65630 12.324488 3.169576 -1.9674069 0.54327917 0.56176667 0.47420000 -0.08756667 0.04982709 0.04982709 FALSE  
ENST00000593829 ENSG00000130520 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM4 protein_coding protein_coding 337.5977 611.5309 184.9882 33.47096 1.598952 -1.724938 21.24413 35.44487 13.05938 3.066952 1.379095 -1.4397938 0.06253333 0.05876667 0.07073333 0.01196667 0.68856938 0.04982709 FALSE  
ENST00000594828 ENSG00000130520 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM4 protein_coding protein_coding 337.5977 611.5309 184.9882 33.47096 1.598952 -1.724938 16.56130 18.22962 13.75537 3.590313 3.953957 -0.4060318 0.05914167 0.02936667 0.07450000 0.04513333 0.11313127 0.04982709 FALSE  
MSTRG.16726.2 ENSG00000130520 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM4 protein_coding   337.5977 611.5309 184.9882 33.47096 1.598952 -1.724938 106.69472 206.57226 67.93526 19.050803 7.242500 -1.6042715 0.31616250 0.33640000 0.36663333 0.03023333 0.80273098 0.04982709 TRUE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130520 E001 0.3729606 0.0283610464 3.460028e-01 4.901497e-01 19 18306236 18306238 3 - 0.210 0.069 -1.863
ENSG00000130520 E002 39.2538830 0.0128341652 2.069019e-01 3.348506e-01 19 18306239 18306333 95 - 1.402 1.522 0.414
ENSG00000130520 E003 88.4532064 0.0172806334 3.604206e-01 5.047316e-01 19 18306334 18306447 114 - 1.896 1.832 -0.213
ENSG00000130520 E004 242.1823951 0.0058518196 6.422216e-01 7.550529e-01 19 18306448 18306906 459 - 2.292 2.274 -0.061
ENSG00000130520 E005 70.3200048 0.0004234940 8.780348e-01 9.256852e-01 19 18306907 18306915 9 - 1.736 1.745 0.031
ENSG00000130520 E006 9731.5502359 0.0010726166 4.885632e-05 2.760007e-04 19 18306916 18307555 640 - 3.836 3.887 0.171
ENSG00000130520 E007 60.5086228 0.0004475166 8.989276e-04 3.610480e-03 19 18309291 18309677 387 - 1.809 1.635 -0.588
ENSG00000130520 E008 2203.5341850 0.0005846078 3.853539e-13 1.008432e-11 19 18309678 18309750 73 - 3.138 3.250 0.372
ENSG00000130520 E009 4431.5957648 0.0002975715 4.290638e-01 5.717925e-01 19 18309751 18309861 111 - 3.525 3.537 0.042
ENSG00000130520 E010 18.9473481 0.0117361092 1.253454e-01 2.276360e-01 19 18310110 18310214 105 - 1.307 1.154 -0.540
ENSG00000130520 E011 36.0228541 0.0009603564 8.199225e-02 1.626682e-01 19 18312169 18312603 435 - 1.554 1.436 -0.406
ENSG00000130520 E012 4261.6505878 0.0007521712 2.699391e-04 1.257417e-03 19 18312604 18312702 99 - 3.552 3.511 -0.138
ENSG00000130520 E013 2975.9647056 0.0005353197 8.730517e-09 1.088067e-07 19 18316024 18316065 42 - 3.416 3.349 -0.223
ENSG00000130520 E014 9.0132172 0.0455147343 5.360933e-01 6.678498e-01 19 18316066 18316259 194 - 0.986 0.889 -0.364
ENSG00000130520 E015 2472.7764896 0.0021168287 1.912605e-04 9.265044e-04 19 18323018 18323258 241 - 3.343 3.268 -0.252