ENSG00000130518

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392413 ENSG00000130518 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCN protein_coding protein_coding 0.262596 0.2138279 0.2778224 0.0609882 0.01508191 0.362789 0.01653828 0.01391865 0.00000000 0.01391865 0.000000000 -1.25813625 0.06765417 0.1145667 0.00000000 -0.1145667 0.694421145 0.003211478 FALSE TRUE
ENST00000599528 ENSG00000130518 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCN protein_coding protein_coding 0.262596 0.2138279 0.2778224 0.0609882 0.01508191 0.362789 0.13573302 0.03943525 0.22213398 0.03943525 0.001126987 2.23134581 0.41947917 0.1198667 0.80433333 0.6844667 0.003211478 0.003211478   FALSE
ENST00000599638 ENSG00000130518 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCN protein_coding retained_intron 0.262596 0.2138279 0.2778224 0.0609882 0.01508191 0.362789 0.09301004 0.13684241 0.03194302 0.03872777 0.009727072 -1.80776618 0.41080000 0.6418333 0.11380000 -0.5280333 0.006899401 0.003211478 FALSE TRUE
ENST00000600328 ENSG00000130518 HEK293_OSMI2_2hA HEK293_TMG_2hB IQCN protein_coding protein_coding 0.262596 0.2138279 0.2778224 0.0609882 0.01508191 0.362789 0.01388481 0.02363154 0.02374536 0.02363154 0.012115655 0.00487398 0.08600417 0.1237333 0.08193333 -0.0418000 0.999689173 0.003211478 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130518 E001 0.2214452 0.043244989 1.00000000   19 18257098 18257098 1 - 0.000 0.068 7.931
ENSG00000130518 E002 4.7897882 0.004376015 0.01456019 0.03949944 19 18257099 18258106 1008 - 0.351 0.707 1.716
ENSG00000130518 E003 10.3744550 0.002462904 0.62546538 0.74158170 19 18258107 18262618 4512 - 0.959 0.938 -0.077
ENSG00000130518 E004 0.2955422 0.035447123 0.39009679   19 18264363 18264923 561 - 0.210 0.068 -1.874
ENSG00000130518 E005 2.0823734 0.290225028 0.18783379 0.31100468 19 18264924 18267089 2166 - 0.674 0.338 -1.659
ENSG00000130518 E006 0.1451727 0.044201810 0.14004768   19 18267090 18267118 29 - 0.210 0.000 -13.481
ENSG00000130518 E007 0.4417591 0.094357388 0.71472760 0.81084982 19 18267119 18267335 217 - 0.210 0.126 -0.885
ENSG00000130518 E008 0.1482932 0.051888738 1.00000000   19 18267336 18267378 43 - 0.000 0.068 9.641
ENSG00000130518 E009 0.1451727 0.044201810 0.14004768   19 18267379 18267522 144 - 0.210 0.000 -13.481
ENSG00000130518 E010 0.1451727 0.044201810 0.14004768   19 18267523 18267526 4 - 0.210 0.000 -13.481
ENSG00000130518 E011 0.0000000       19 18267527 18267565 39 -      
ENSG00000130518 E012 0.0000000       19 18267772 18267967 196 -      
ENSG00000130518 E013 0.0000000       19 18269466 18269587 122 -      
ENSG00000130518 E014 0.0000000       19 18269588 18269591 4 -      
ENSG00000130518 E015 0.0000000       19 18274403 18274411 9 -      
ENSG00000130518 E016 0.0000000       19 18274412 18274430 19 -      
ENSG00000130518 E017 0.0000000       19 18274431 18274452 22 -      
ENSG00000130518 E018 0.0000000       19 18274453 18274471 19 -      
ENSG00000130518 E019 0.0000000       19 18274472 18274487 16 -      
ENSG00000130518 E020 0.0000000       19 18274488 18274500 13 -