ENSG00000130477

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000519716 ENSG00000130477 HEK293_OSMI2_2hA HEK293_TMG_2hB UNC13A protein_coding protein_coding 1.03338 0.6695867 2.183451 0.04400364 0.007953478 1.690473 0.86931670 0.6392168 1.7268485 0.03441081 0.06472661 1.419700 0.88224167 0.9561667 0.7908333 -0.1653333 0.01034439 0.01034439 FALSE TRUE
MSTRG.16698.2 ENSG00000130477 HEK293_OSMI2_2hA HEK293_TMG_2hB UNC13A protein_coding   1.03338 0.6695867 2.183451 0.04400364 0.007953478 1.690473 0.02155952 0.0000000 0.1667975 0.00000000 0.06478408 4.144026 0.01035417 0.0000000 0.0764000 0.0764000 0.14129879 0.01034439 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130477 E001 75.0605413 0.0192966429 5.731229e-08 6.097059e-07 19 17601336 17602887 1552 - 1.663 2.106 1.493
ENSG00000130477 E002 21.1609551 0.0009212564 2.889400e-04 1.334898e-03 19 17602888 17603087 200 - 1.175 1.515 1.186
ENSG00000130477 E003 101.7535539 0.0189577791 1.067414e-03 4.192269e-03 19 17603088 17605320 2233 - 1.863 2.134 0.910
ENSG00000130477 E004 18.6287165 0.0009889461 6.540649e-01 7.643567e-01 19 17605321 17605960 640 - 1.226 1.251 0.087
ENSG00000130477 E005 1.8737910 0.0079399154 5.292225e-01 6.620344e-01 19 17605961 17606050 90 - 0.432 0.351 -0.449
ENSG00000130477 E006 1.9039799 0.0258093326 9.446199e-01 9.692440e-01 19 17606051 17606128 78 - 0.403 0.458 0.291
ENSG00000130477 E007 1.3837703 0.2899314784 9.504401e-01 9.729711e-01 19 17606129 17606131 3 - 0.339 0.353 0.081
ENSG00000130477 E008 4.7300515 0.2105772843 3.661364e-01 5.104682e-01 19 17606132 17606313 182 - 0.746 0.548 -0.850
ENSG00000130477 E009 5.4844064 0.0284144341 1.663327e-01 2.834305e-01 19 17606314 17606354 41 - 0.797 0.615 -0.758
ENSG00000130477 E010 6.6377582 0.0025530297 3.780925e-01 5.223844e-01 19 17609940 17609974 35 - 0.842 0.777 -0.258
ENSG00000130477 E011 11.7410844 0.0017449294 8.809292e-01 9.276632e-01 19 17609975 17610099 125 - 1.029 1.108 0.286
ENSG00000130477 E012 8.9701714 0.0019249186 9.838711e-01 9.939869e-01 19 17611763 17611855 93 - 0.929 0.987 0.219
ENSG00000130477 E013 0.0000000       19 17611856 17612160 305 -      
ENSG00000130477 E014 0.0000000       19 17616408 17616464 57 -      
ENSG00000130477 E015 13.6161495 0.0013426560 5.171060e-01 6.515568e-01 19 17617702 17617849 148 - 1.111 1.108 -0.010
ENSG00000130477 E016 11.5761711 0.0015419781 1.320522e-01 2.369697e-01 19 17618421 17618501 81 - 1.069 0.959 -0.406
ENSG00000130477 E017 8.8877716 0.0025405662 3.389577e-01 4.828595e-01 19 17618906 17618962 57 - 0.954 0.895 -0.224
ENSG00000130477 E018 0.0000000       19 17618963 17619042 80 -      
ENSG00000130477 E019 4.1384289 0.0038527905 1.777441e-02 4.665218e-02 19 17620693 17620722 30 - 0.734 0.351 -1.830
ENSG00000130477 E020 3.3967595 0.0045830600 9.531539e-03 2.761549e-02 19 17621832 17621870 39 - 0.676 0.210 -2.588
ENSG00000130477 E021 2.9740406 0.0070643704 2.102874e-02 5.364924e-02 19 17623542 17623547 6 - 0.627 0.210 -2.378
ENSG00000130477 E022 8.4729673 0.1381822192 2.638169e-01 4.017308e-01 19 17624829 17624952 124 - 0.946 0.826 -0.458
ENSG00000130477 E023 0.0000000       19 17626288 17626632 345 -      
ENSG00000130477 E024 7.4723008 0.0483073542 3.909221e-01 5.348651e-01 19 17626633 17626785 153 - 0.892 0.819 -0.281
ENSG00000130477 E025 6.2145482 0.0079770372 2.812882e-01 4.211854e-01 19 17627509 17627597 89 - 0.831 0.729 -0.409
ENSG00000130477 E026 6.9916034 0.0034183395 6.907066e-01 7.923383e-01 19 17627863 17627940 78 - 0.853 0.859 0.023
ENSG00000130477 E027 1.0298247 0.0125372644 5.233132e-01 6.570183e-01 19 17627941 17628337 397 - 0.305 0.210 -0.715
ENSG00000130477 E028 7.7911140 0.0021850035 5.426144e-02 1.167007e-01 19 17629240 17629323 84 - 0.937 0.729 -0.814
ENSG00000130477 E029 9.3698553 0.0099308750 3.442320e-02 8.056847e-02 19 17630145 17630288 144 - 1.008 0.776 -0.886
ENSG00000130477 E030 6.9987428 0.0260792500 9.421853e-02 1.817271e-01 19 17630654 17630750 97 - 0.893 0.675 -0.867
ENSG00000130477 E031 8.8179279 0.0048902876 3.868106e-01 5.309752e-01 19 17632782 17632908 127 - 0.946 0.895 -0.192
ENSG00000130477 E032 8.1990671 0.0125988871 5.869499e-01 7.103717e-01 19 17633108 17633193 86 - 0.911 0.895 -0.059
ENSG00000130477 E033 10.0498667 0.0022791621 8.261542e-01 8.905960e-01 19 17636024 17636157 134 - 0.978 1.014 0.135
ENSG00000130477 E034 8.8621732 0.0175204602 3.788003e-01 5.230943e-01 19 17639083 17639217 135 - 0.954 0.895 -0.223
ENSG00000130477 E035 6.4089511 0.0030389172 4.223244e-01 5.654562e-01 19 17639436 17639525 90 - 0.832 0.777 -0.216
ENSG00000130477 E036 4.6729329 0.0036929853 2.738252e-01 4.129188e-01 19 17639840 17639908 69 - 0.734 0.614 -0.507
ENSG00000130477 E037 8.8770502 0.0025481174 1.766385e-01 2.968765e-01 19 17640511 17640661 151 - 0.970 0.859 -0.422
ENSG00000130477 E038 8.7027208 0.0018972372 4.909564e-02 1.075420e-01 19 17641393 17641556 164 - 0.978 0.777 -0.773
ENSG00000130477 E039 8.4440614 0.0046559219 1.634869e-01 2.796929e-01 19 17642845 17642960 116 - 0.946 0.820 -0.483
ENSG00000130477 E040 13.9184477 0.0245233371 4.940850e-01 6.310749e-01 19 17645674 17645843 170 - 1.117 1.089 -0.101
ENSG00000130477 E041 9.3379050 0.0020098420 2.404029e-01 3.746133e-01 19 17645970 17646111 142 - 0.892 1.087 0.720
ENSG00000130477 E042 8.6992590 0.0191219329 3.158059e-01 4.585766e-01 19 17647265 17647492 228 - 0.946 0.860 -0.327
ENSG00000130477 E043 8.7346237 0.0020576629 7.219609e-03 2.179481e-02 19 17648431 17648650 220 - 0.993 0.675 -1.245
ENSG00000130477 E044 6.4428181 0.0025862172 3.794641e-02 8.724948e-02 19 17648912 17648983 72 - 0.873 0.614 -1.055
ENSG00000130477 E045 1.4811488 0.0091320969 7.496248e-01 8.365190e-01 19 17649339 17649344 6 - 0.373 0.351 -0.130
ENSG00000130477 E046 4.7974685 0.0032719242 3.151043e-02 7.490033e-02 19 17649509 17649587 79 - 0.773 0.457 -1.402
ENSG00000130477 E047 3.5419176 0.0060056125 5.647479e-02 1.205509e-01 19 17652631 17652677 47 - 0.660 0.351 -1.520
ENSG00000130477 E048 4.9813510 0.0032469774 5.578608e-03 1.749695e-02 19 17655274 17655382 109 - 0.798 0.351 -2.082
ENSG00000130477 E049 15.5471574 0.0013141059 2.832812e-06 2.132172e-05 19 17655883 17656398 516 - 1.239 0.729 -1.906
ENSG00000130477 E050 8.6858209 0.0372721133 3.182144e-01 4.611748e-01 19 17658062 17658269 208 - 0.955 0.857 -0.373
ENSG00000130477 E051 3.7459109 0.1253106892 6.833385e-01 7.865651e-01 19 17663532 17663567 36 - 0.644 0.606 -0.165
ENSG00000130477 E052 4.2321031 0.0091067022 7.306589e-01 8.225360e-01 19 17666650 17666704 55 - 0.676 0.675 -0.005
ENSG00000130477 E053 4.2280522 0.0268626148 2.346816e-01 3.679924e-01 19 17668117 17668190 74 - 0.706 0.543 -0.713
ENSG00000130477 E054 4.9415084 0.0032868749 8.209223e-02 1.628307e-01 19 17669553 17669676 124 - 0.773 0.543 -0.987
ENSG00000130477 E055 4.2104481 0.0037699376 7.051307e-02 1.441916e-01 19 17672378 17672495 118 - 0.720 0.457 -1.188
ENSG00000130477 E056 3.5577261 0.0162259369 6.736398e-04 2.802289e-03 19 17674657 17674756 100 - 0.706 0.000 -14.686
ENSG00000130477 E057 0.1515154 0.0424325354 1.000000e+00   19 17676012 17676041 30 - 0.068 0.000 -10.432
ENSG00000130477 E058 3.3935252 0.0143217445 8.570571e-04 3.462645e-03 19 17688178 17688365 188 - 0.691 0.000 -14.633