ENSG00000130402

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000252699 ENSG00000130402 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN4 protein_coding protein_coding 142.3814 177.4311 107.1756 8.310961 1.686135 -0.7272291 24.804612 41.51876 11.6446482 0.7546987 0.35297331 -1.83320540 0.16495833 0.23546667 0.108800000 -0.126666667 5.023668e-09 5.023668e-09 FALSE TRUE
ENST00000390009 ENSG00000130402 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN4 protein_coding protein_coding 142.3814 177.4311 107.1756 8.310961 1.686135 -0.7272291 9.395743 26.15433 0.2998582 2.1786993 0.29985816 -6.39984967 0.05625417 0.14760000 0.002733333 -0.144866667 2.123626e-03 5.023668e-09 FALSE TRUE
ENST00000424234 ENSG00000130402 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN4 protein_coding protein_coding 142.3814 177.4311 107.1756 8.310961 1.686135 -0.7272291 18.639010 15.87537 16.3182930 2.4665698 0.30306866 0.03967535 0.13512917 0.08856667 0.152366667 0.063800000 5.406313e-03 5.023668e-09 FALSE TRUE
ENST00000497637 ENSG00000130402 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN4 protein_coding retained_intron 142.3814 177.4311 107.1756 8.310961 1.686135 -0.7272291 7.608765 20.24969 1.0669198 6.0519336 0.08114531 -4.23362971 0.04839583 0.11703333 0.009966667 -0.107066667 4.018317e-05 5.023668e-09 FALSE TRUE
MSTRG.17094.1 ENSG00000130402 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN4 protein_coding   142.3814 177.4311 107.1756 8.310961 1.686135 -0.7272291 13.637295 18.80600 10.6180456 1.4489561 0.77323269 -0.82408337 0.09446250 0.10636667 0.099300000 -0.007066667 8.715088e-01 5.023668e-09 FALSE TRUE
MSTRG.17094.2 ENSG00000130402 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN4 protein_coding   142.3814 177.4311 107.1756 8.310961 1.686135 -0.7272291 38.211721 34.02832 37.6338976 8.3804861 2.66896486 0.14525599 0.27647083 0.18833333 0.350566667 0.162233333 5.025338e-02 5.023668e-09 FALSE TRUE
MSTRG.17094.9 ENSG00000130402 HEK293_OSMI2_2hA HEK293_TMG_2hB ACTN4 protein_coding   142.3814 177.4311 107.1756 8.310961 1.686135 -0.7272291 20.448156 16.24346 21.3147492 2.7131453 1.00918206 0.39178164 0.15015417 0.09093333 0.198700000 0.107766667 3.311360e-05 5.023668e-09 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130402 E001 6.3740338 9.318928e-02 1.534158e-01 2.663212e-01 19 38647641 38647643 3 + 0.991 0.747 -0.940
ENSG00000130402 E002 8.1390674 6.080606e-02 1.464943e-01 2.569190e-01 19 38647644 38647648 5 + 1.060 0.854 -0.771
ENSG00000130402 E003 11.7939135 3.900112e-02 1.277995e-02 3.539705e-02 19 38647649 38647659 11 + 1.250 0.953 -1.076
ENSG00000130402 E004 17.5918660 9.619763e-03 3.669404e-05 2.134153e-04 19 38647660 38647669 10 + 1.439 1.085 -1.245
ENSG00000130402 E005 194.4640096 1.520303e-02 2.895276e-02 6.991375e-02 19 38647670 38647737 68 + 2.330 2.226 -0.345
ENSG00000130402 E006 640.4020082 5.917896e-03 5.225963e-03 1.654567e-02 19 38647738 38647907 170 + 2.831 2.753 -0.260
ENSG00000130402 E007 0.3268771 2.926935e-02 6.761002e-01   19 38648193 38648260 68 + 0.153 0.098 -0.739
ENSG00000130402 E008 860.3508126 3.857311e-03 6.320321e-05 3.471229e-04 19 38700600 38700714 115 + 2.971 2.874 -0.324
ENSG00000130402 E009 1057.4666735 8.050626e-04 9.719756e-13 2.392022e-11 19 38701002 38701121 120 + 3.058 2.964 -0.315
ENSG00000130402 E010 1020.3593137 7.205747e-04 1.067772e-13 3.021903e-12 19 38704934 38705020 87 + 3.045 2.947 -0.324
ENSG00000130402 E011 994.6447839 1.541393e-04 2.720502e-26 3.316543e-24 19 38706044 38706131 88 + 3.038 2.931 -0.357
ENSG00000130402 E012 930.0815056 5.136058e-04 1.365114e-14 4.410244e-13 19 38708117 38708195 79 + 3.002 2.908 -0.314
ENSG00000130402 E013 955.6742425 1.139250e-04 9.742212e-25 1.038623e-22 19 38709395 38709476 82 + 3.017 2.916 -0.338
ENSG00000130402 E014 853.8674295 2.806604e-04 6.312193e-12 1.357659e-10 19 38710257 38710342 86 + 2.951 2.878 -0.242
ENSG00000130402 E015 32.4387849 1.013220e-02 6.700277e-04 2.788733e-03 19 38710833 38711275 443 + 1.634 1.398 -0.809
ENSG00000130402 E016 65.7244847 1.853873e-03 1.929956e-08 2.257777e-07 19 38711276 38711361 86 + 1.938 1.695 -0.820
ENSG00000130402 E017 870.1835785 8.095430e-04 8.898425e-06 5.986860e-05 19 38714469 38714561 93 + 2.946 2.894 -0.174
ENSG00000130402 E018 1248.2362201 1.314083e-03 1.213381e-02 3.385192e-02 19 38717086 38717316 231 + 3.082 3.063 -0.065
ENSG00000130402 E019 1233.6502470 1.531228e-03 1.432181e-02 3.896178e-02 19 38717927 38718074 148 + 3.078 3.057 -0.069
ENSG00000130402 E020 1314.2681994 2.999864e-04 5.364087e-05 2.999440e-04 19 38721538 38721688 151 + 3.106 3.082 -0.079
ENSG00000130402 E021 2.3186796 4.012921e-02 7.076676e-01 8.055165e-01 19 38721689 38721878 190 + 0.428 0.517 0.443
ENSG00000130402 E022 1066.2987166 4.457818e-04 3.461178e-02 8.092385e-02 19 38723614 38723722 109 + 3.002 2.998 -0.010
ENSG00000130402 E023 1067.3096569 3.167332e-04 1.593327e-01 2.742255e-01 19 38723937 38724077 141 + 2.994 3.003 0.028
ENSG00000130402 E024 1315.6918291 8.131436e-05 2.044483e-02 5.242714e-02 19 38724157 38724339 183 + 3.054 3.107 0.175
ENSG00000130402 E025 1173.6728937 8.198526e-05 2.068623e-06 1.604021e-05 19 38724431 38724565 135 + 2.984 3.066 0.273
ENSG00000130402 E026 3.7839312 4.223009e-03 6.796467e-01 7.837640e-01 19 38725589 38725723 135 + 0.596 0.680 0.362
ENSG00000130402 E027 543.5064687 1.429582e-04 8.553007e-04 3.456434e-03 19 38725724 38725726 3 + 2.649 2.733 0.279
ENSG00000130402 E028 1386.4192914 5.401080e-04 1.296148e-02 3.582024e-02 19 38725727 38725903 177 + 3.069 3.135 0.218
ENSG00000130402 E029 1328.7737623 4.654682e-04 1.806437e-04 8.807495e-04 19 38726957 38727103 147 + 3.039 3.122 0.274
ENSG00000130402 E030 13.0118552 1.345033e-03 1.788141e-01 2.996532e-01 19 38727856 38727945 90 + 1.184 1.082 -0.366
ENSG00000130402 E031 1107.6741132 4.849478e-04 3.412514e-06 2.523481e-05 19 38727946 38728026 81 + 2.947 3.047 0.333
ENSG00000130402 E032 5.1689896 1.379176e-02 2.757031e-03 9.543592e-03 19 38728145 38728315 171 + 0.992 0.604 -1.548
ENSG00000130402 E033 19.6208861 7.205856e-03 1.291004e-01 2.329303e-01 19 38728316 38728381 66 + 1.359 1.252 -0.374
ENSG00000130402 E034 1581.8080697 1.941388e-03 1.967730e-04 9.500427e-04 19 38728996 38729154 159 + 3.093 3.207 0.380
ENSG00000130402 E035 3215.8897013 2.359397e-03 5.816308e-16 2.267247e-14 19 38729274 38729988 715 + 3.327 3.541 0.712
ENSG00000130402 E036 1120.8950070 7.720746e-03 9.138863e-17 3.957326e-15 19 38729989 38731589 1601 + 2.718 3.121 1.340