ENSG00000130363

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000367069 ENSG00000130363 HEK293_OSMI2_2hA HEK293_TMG_2hB RSPH3 protein_coding protein_coding 3.459302 3.065202 3.624274 0.1116013 0.0323826 0.240986 1.2725479 0.51120806 1.8187197 0.17213129 0.12341673 1.8109027 0.3441917 0.171466667 0.50126667 0.32980000 0.005664708 0.003620803 FALSE TRUE
ENST00000449822 ENSG00000130363 HEK293_OSMI2_2hA HEK293_TMG_2hB RSPH3 protein_coding protein_coding 3.459302 3.065202 3.624274 0.1116013 0.0323826 0.240986 0.3051574 0.19346247 0.2962784 0.10319653 0.15300524 0.5900807 0.0891000 0.063733333 0.08243333 0.01870000 0.997690392 0.003620803 FALSE TRUE
MSTRG.29266.1 ENSG00000130363 HEK293_OSMI2_2hA HEK293_TMG_2hB RSPH3 protein_coding   3.459302 3.065202 3.624274 0.1116013 0.0323826 0.240986 1.7509363 2.33979135 1.2948815 0.30716081 0.15069174 -0.8486138 0.5342417 0.758300000 0.35700000 -0.40130000 0.003620803 0.003620803 TRUE TRUE
MSTRG.29266.3 ENSG00000130363 HEK293_OSMI2_2hA HEK293_TMG_2hB RSPH3 protein_coding   3.459302 3.065202 3.624274 0.1116013 0.0323826 0.240986 0.1306603 0.02073984 0.2143941 0.02073984 0.07051168 2.8678531 0.0324500 0.006533333 0.05930000 0.05276667 0.075222464 0.003620803 TRUE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130363 E001 1.326422 0.010263856 4.680759e-02 1.034793e-01 6 158972494 158972593 100 - 0.184 0.533 2.198
ENSG00000130363 E002 82.746726 0.080989012 1.830784e-02 4.780523e-02 6 158972871 158975390 2520 - 2.010 1.779 -0.779
ENSG00000130363 E003 21.540998 0.001018604 1.237496e-10 2.150824e-09 6 158975391 158975922 532 - 1.516 0.994 -1.846
ENSG00000130363 E004 16.310978 0.001144658 2.488943e-05 1.510447e-04 6 158975923 158976581 659 - 1.364 0.994 -1.319
ENSG00000130363 E005 9.814490 0.002076300 7.294859e-03 2.198834e-02 6 158976582 158976833 252 - 1.134 0.847 -1.064
ENSG00000130363 E006 10.948152 0.016776040 4.016600e-04 1.780876e-03 6 158976834 158977148 315 - 1.218 0.793 -1.577
ENSG00000130363 E007 12.902407 0.001661170 7.098107e-01 8.070937e-01 6 158977149 158977482 334 - 1.126 1.167 0.146
ENSG00000130363 E008 53.837492 0.006739166 1.209061e-01 2.212447e-01 6 158977483 158977848 366 - 1.696 1.787 0.307
ENSG00000130363 E009 20.542900 0.001050364 9.516070e-01 9.737015e-01 6 158978260 158978346 87 - 1.322 1.333 0.039
ENSG00000130363 E010 33.329936 0.007027793 4.432481e-02 9.897809e-02 6 158980774 158980936 163 - 1.465 1.607 0.485
ENSG00000130363 E011 41.980761 0.003633370 7.184592e-01 8.135486e-01 6 158982485 158982688 204 - 1.621 1.647 0.090
ENSG00000130363 E012 25.632258 0.004636140 5.374981e-01 6.690501e-01 6 158983662 158983807 146 - 1.402 1.452 0.175
ENSG00000130363 E013 18.737576 0.001373427 8.732911e-02 1.710884e-01 6 158986280 158986421 142 - 1.225 1.364 0.488
ENSG00000130363 E014 14.991152 0.009430054 1.611542e-01 2.766091e-01 6 158993839 158993926 88 - 1.257 1.129 -0.457
ENSG00000130363 E015 78.948531 0.019109782 5.778193e-08 6.141157e-07 6 158999435 159000202 768 - 1.700 2.063 1.222