ENSG00000130348

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000369046 ENSG00000130348 HEK293_OSMI2_2hA HEK293_TMG_2hB QRSL1 protein_coding protein_coding 11.49754 3.8849 18.01986 0.3424214 0.3657219 2.21073 7.545461 2.9632645 10.856582 0.3355859 0.2843296 1.869778 0.6982750 0.7605667 0.6024 -0.1581667 0.005159019 0.005159019 FALSE TRUE
MSTRG.28720.3 ENSG00000130348 HEK293_OSMI2_2hA HEK293_TMG_2hB QRSL1 protein_coding   11.49754 3.8849 18.01986 0.3424214 0.3657219 2.21073 3.488897 0.8961639 6.389413 0.1245998 0.2837563 2.820096 0.2584042 0.2329000 0.3544 0.1215000 0.061563465 0.005159019 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130348 E001 10.6832146 0.0052313543 1.767691e-02 4.643940e-02 6 106629578 106629598 21 + 1.094 0.790 -1.142
ENSG00000130348 E002 24.1533261 0.0008115585 1.000969e-03 3.961595e-03 6 106629599 106629613 15 + 1.422 1.135 -1.005
ENSG00000130348 E003 66.5062252 0.0003789918 4.301452e-03 1.398869e-02 6 106629614 106629705 92 + 1.821 1.677 -0.484
ENSG00000130348 E004 0.4439371 0.0215702972 4.707903e-01 6.099950e-01 6 106640101 106640206 106 + 0.191 0.000 -9.467
ENSG00000130348 E005 115.6906121 0.0002494031 2.028923e-04 9.762608e-04 6 106640349 106640508 160 + 2.057 1.917 -0.469
ENSG00000130348 E006 97.2346809 0.0003156862 1.669035e-02 4.425569e-02 6 106640823 106640921 99 + 1.973 1.876 -0.325
ENSG00000130348 E007 85.5849900 0.0005391685 2.476292e-01 3.831603e-01 6 106642994 106643090 97 + 1.908 1.859 -0.165
ENSG00000130348 E008 4.1716325 0.0038431868 1.131406e-01 2.101119e-01 6 106643896 106644033 138 + 0.596 0.830 0.966
ENSG00000130348 E009 94.6261313 0.0057215144 6.270231e-01 7.428917e-01 6 106649025 106649201 177 + 1.931 1.950 0.066
ENSG00000130348 E010 98.6029697 0.0003092288 9.903641e-01 9.981089e-01 6 106652209 106652384 176 + 1.955 1.955 0.001
ENSG00000130348 E011 89.7460717 0.0003093222 9.499826e-01 9.726904e-01 6 106652467 106652582 116 + 1.916 1.914 -0.005
ENSG00000130348 E012 6.5912131 0.0025714515 9.880762e-03 2.847227e-02 6 106652583 106653823 1241 + 0.922 0.519 -1.678
ENSG00000130348 E013 105.2640919 0.0002916339 3.430515e-03 1.152552e-02 6 106654730 106654922 193 + 1.953 2.060 0.359
ENSG00000130348 E014 70.2581892 0.0003743569 7.321563e-05 3.956438e-04 6 106655615 106655732 118 + 1.754 1.929 0.590
ENSG00000130348 E015 107.1714168 0.0002754222 5.772733e-06 4.051671e-05 6 106662980 106663185 206 + 1.940 2.102 0.541
ENSG00000130348 E016 311.7978907 0.0007295913 7.642388e-01 8.473662e-01 6 106665782 106668417 2636 + 2.449 2.456 0.022