ENSG00000130332

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000252622 ENSG00000130332 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM7 protein_coding protein_coding 439.1921 836.8276 210.5875 7.213204 3.358534 -1.990459 345.22960 663.3837 148.25543 7.662481 2.1152670 -2.161683 0.7742417 0.7927667 0.7040667 -0.08870000 1.503066e-07 1.624979e-14 FALSE  
ENST00000585409 ENSG00000130332 HEK293_OSMI2_2hA HEK293_TMG_2hB LSM7 protein_coding protein_coding 439.1921 836.8276 210.5875 7.213204 3.358534 -1.990459 55.07275 110.8184 34.10506 3.086928 0.5121139 -1.699847 0.1274958 0.1324333 0.1619667 0.02953333 2.636022e-03 1.624979e-14    
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130332 E001 0.920137 1.364625e-02 9.310119e-01 9.605682e-01 19 2321520 2321520 1 - 0.228 0.208 -0.166
ENSG00000130332 E002 4268.107698 5.393915e-05 5.153243e-07 4.546695e-06 19 2321521 2321822 302 - 3.464 3.496 0.104
ENSG00000130332 E003 3332.246575 7.170459e-05 1.171553e-01 2.158809e-01 19 2324125 2324192 68 - 3.376 3.385 0.032
ENSG00000130332 E004 1542.863847 1.793668e-04 7.467589e-01 8.344380e-01 19 2324193 2324196 4 - 3.049 3.050 0.004
ENSG00000130332 E005 105.092402 3.059649e-04 1.330283e-13 3.714891e-12 19 2324197 2324532 336 - 2.123 1.824 -1.004
ENSG00000130332 E006 105.072502 7.512858e-04 2.132704e-13 5.785344e-12 19 2324742 2324912 171 - 2.130 1.827 -1.018
ENSG00000130332 E007 50.347289 4.684054e-04 3.334969e-18 1.720588e-16 19 2325980 2326139 160 - 1.927 1.457 -1.595
ENSG00000130332 E008 40.285585 9.948015e-04 2.532184e-38 7.851305e-36 19 2327962 2328386 425 - 1.989 1.249 -2.529
ENSG00000130332 E009 2588.695003 6.067747e-05 1.835167e-04 8.929187e-04 19 2328387 2328411 25 - 3.247 3.279 0.105
ENSG00000130332 E010 2643.028201 5.949090e-05 3.569715e-01 5.013877e-01 19 2328412 2328477 66 - 3.292 3.281 -0.037
ENSG00000130332 E011 113.924408 1.573642e-02 1.897677e-01 3.134700e-01 19 2328478 2328560 83 - 2.004 1.902 -0.342
ENSG00000130332 E012 1263.895309 1.555474e-03 5.049910e-03 1.606815e-02 19 2328561 2328611 51 - 3.015 2.951 -0.215