ENSG00000130311

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000359866 ENSG00000130311 HEK293_OSMI2_2hA HEK293_TMG_2hB DDA1 protein_coding protein_coding 25.17464 40.32699 19.75 2.751146 0.6632724 -1.029521 18.658266 33.174435 12.266495 1.0277499 0.2835473 -1.4346079 0.71911667 0.8270 0.6222667 -0.20473333 0.002596037 0.002596037 FALSE TRUE
ENST00000593466 ENSG00000130311 HEK293_OSMI2_2hA HEK293_TMG_2hB DDA1 protein_coding nonsense_mediated_decay 25.17464 40.32699 19.75 2.751146 0.6632724 -1.029521 2.538257 3.144405 2.839877 0.3124455 0.3678746 -0.1464681 0.11035833 0.0779 0.1444333 0.06653333 0.035998445 0.002596037 TRUE TRUE
ENST00000594501 ENSG00000130311 HEK293_OSMI2_2hA HEK293_TMG_2hB DDA1 protein_coding retained_intron 25.17464 40.32699 19.75 2.751146 0.6632724 -1.029521 2.191221 1.938832 2.587067 0.8961515 0.5882144 0.4142738 0.09181667 0.0454 0.1295000 0.08410000 0.190725485 0.002596037 FALSE TRUE
ENST00000596925 ENSG00000130311 HEK293_OSMI2_2hA HEK293_TMG_2hB DDA1 protein_coding retained_intron 25.17464 40.32699 19.75 2.751146 0.6632724 -1.029521 1.309040 1.295280 1.723641 0.5923341 0.4606506 0.4094458 0.05922083 0.0308 0.0866000 0.05580000 0.100035589 0.002596037 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130311 E001 1.396351 0.0098092775 3.375672e-01 4.814506e-01 19 17309445 17309517 73 + 0.187 0.376 1.357
ENSG00000130311 E002 69.694425 0.0004788817 1.137431e-04 5.849634e-04 19 17309518 17309562 45 + 1.901 1.729 -0.579
ENSG00000130311 E003 80.099804 0.0019933405 3.947322e-04 1.754348e-03 19 17309563 17309568 6 + 1.953 1.792 -0.543
ENSG00000130311 E004 87.024393 0.0010997479 4.351627e-04 1.909710e-03 19 17309569 17309573 5 + 1.978 1.831 -0.495
ENSG00000130311 E005 388.913787 0.0002052027 4.305405e-01 5.731836e-01 19 17309574 17309657 84 + 2.522 2.511 -0.035
ENSG00000130311 E006 311.290147 0.0002431184 5.986124e-02 1.263780e-01 19 17314023 17314026 4 + 2.377 2.428 0.171
ENSG00000130311 E007 627.454978 0.0001943416 6.388275e-01 7.523932e-01 19 17314027 17314103 77 + 2.711 2.725 0.047
ENSG00000130311 E008 616.707360 0.0001367223 9.307463e-01 9.604186e-01 19 17314338 17314389 52 + 2.709 2.716 0.025
ENSG00000130311 E009 11.389401 0.0397094047 5.635418e-03 1.764504e-02 19 17314390 17314732 343 + 1.261 0.904 -1.299
ENSG00000130311 E010 8.111395 0.0265466330 6.710011e-04 2.792524e-03 19 17315772 17315933 162 + 1.176 0.731 -1.672
ENSG00000130311 E011 635.453139 0.0002140977 3.557012e-01 5.000532e-01 19 17315934 17315995 62 + 2.712 2.734 0.072
ENSG00000130311 E012 694.310322 0.0003060371 1.607066e-02 4.289291e-02 19 17319546 17319725 180 + 2.731 2.778 0.156
ENSG00000130311 E013 838.042891 0.0012034255 1.039113e-09 1.536264e-08 19 17319726 17320250 525 + 2.744 2.877 0.442
ENSG00000130311 E014 139.575965 0.0074158509 5.558292e-01 6.846299e-01 19 17320251 17320329 79 + 2.092 2.069 -0.077
ENSG00000130311 E015 1209.371437 0.0001170885 6.609275e-11 1.201177e-09 19 17320330 17323298 2969 + 3.056 2.991 -0.216