ENSG00000130309

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000252599 ENSG00000130309 HEK293_OSMI2_2hA HEK293_TMG_2hB COLGALT1 protein_coding protein_coding 103.3705 145.4439 71.53996 6.749872 0.9618616 -1.023539 30.44800 24.70618 38.211731 4.081892 1.112502 0.6289376 0.3145833 0.1686333 0.5345 0.36586667 3.453449e-13 1.354447e-33 FALSE TRUE
ENST00000593832 ENSG00000130309 HEK293_OSMI2_2hA HEK293_TMG_2hB COLGALT1 protein_coding retained_intron 103.3705 145.4439 71.53996 6.749872 0.9618616 -1.023539 12.85551 16.46562 9.282192 1.324902 0.255197 -0.8262416 0.1248792 0.1142333 0.1299 0.01566667 7.016125e-01 1.354447e-33 FALSE TRUE
MSTRG.16696.10 ENSG00000130309 HEK293_OSMI2_2hA HEK293_TMG_2hB COLGALT1 protein_coding   103.3705 145.4439 71.53996 6.749872 0.9618616 -1.023539 55.39096 97.65845 20.499394 4.352377 1.120359 -2.2516076 0.5132042 0.6719000 0.2863 -0.38560000 1.354447e-33 1.354447e-33 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130309 E001 33.3939334 0.0007217705 3.066704e-48 1.703747e-45 19 17555649 17555713 65 + 1.872 1.042 -2.874
ENSG00000130309 E002 48.2873099 0.0004447108 2.783985e-66 3.612449e-63 19 17555714 17555800 87 + 2.023 1.208 -2.784
ENSG00000130309 E003 184.8385989 0.0046632575 3.187810e-24 3.241659e-22 19 17555801 17555973 173 + 2.428 2.107 -1.073
ENSG00000130309 E004 1.1394198 0.0841536770 6.060564e-01 7.263103e-01 19 17556561 17556679 119 + 0.350 0.307 -0.271
ENSG00000130309 E005 1.2115396 0.0120284869 8.090281e-01 8.786941e-01 19 17557322 17557329 8 + 0.261 0.359 0.639
ENSG00000130309 E006 2.7334588 0.0054479521 3.714898e-01 5.159309e-01 19 17557330 17557444 115 + 0.590 0.520 -0.320
ENSG00000130309 E007 389.8080687 0.0019700064 4.430490e-13 1.151204e-11 19 17559311 17559421 111 + 2.627 2.531 -0.319
ENSG00000130309 E008 547.8800104 0.0002072096 3.170773e-16 1.281647e-14 19 17560348 17560465 118 + 2.733 2.700 -0.109
ENSG00000130309 E009 0.3268771 0.0274424043 6.022514e-01   19 17566033 17566183 151 + 0.150 0.099 -0.683
ENSG00000130309 E010 717.3255760 0.0010908228 4.240738e-10 6.729165e-09 19 17567406 17567540 135 + 2.844 2.823 -0.070
ENSG00000130309 E011 823.7395391 0.0017611973 8.370801e-08 8.629477e-07 19 17568509 17568709 201 + 2.901 2.885 -0.054
ENSG00000130309 E012 233.6818517 0.0032274499 3.429403e-03 1.152280e-02 19 17568710 17568713 4 + 2.343 2.343 0.000
ENSG00000130309 E013 8.6420324 0.0049962729 6.538682e-01 7.642258e-01 19 17571870 17571987 118 + 0.926 0.965 0.149
ENSG00000130309 E014 4.1726847 0.0038583399 7.004331e-01 7.999142e-01 19 17571988 17571991 4 + 0.590 0.730 0.597
ENSG00000130309 E015 564.3802854 0.0040665681 9.602125e-02 1.843988e-01 19 17572483 17572602 120 + 2.692 2.744 0.175
ENSG00000130309 E016 3.6537121 0.0045423770 3.983502e-02 9.072147e-02 19 17572603 17573798 1196 + 0.774 0.553 -0.946
ENSG00000130309 E017 2.9182096 0.0502073757 9.504278e-01 9.729711e-01 19 17577075 17577194 120 + 0.541 0.608 0.301
ENSG00000130309 E018 533.8712430 0.0018444704 1.331299e-03 5.080386e-03 19 17577195 17577271 77 + 2.685 2.710 0.082
ENSG00000130309 E019 547.7318563 0.0024312830 2.292855e-02 5.759696e-02 19 17577361 17577467 107 + 2.684 2.728 0.147
ENSG00000130309 E020 617.1534145 0.0025973992 6.019748e-02 1.269427e-01 19 17577957 17578089 133 + 2.729 2.784 0.184
ENSG00000130309 E021 738.1075297 0.0013635379 1.322605e-01 2.372627e-01 19 17579482 17579609 128 + 2.792 2.867 0.248
ENSG00000130309 E022 180.6537313 0.0002128031 1.279078e-33 2.824213e-31 19 17579951 17580698 748 + 2.365 2.142 -0.746
ENSG00000130309 E023 1101.9805387 0.0003835188 3.151160e-03 1.071081e-02 19 17580699 17580903 205 + 2.972 3.036 0.214
ENSG00000130309 E024 410.5781406 0.0018760444 2.227991e-02 5.625340e-02 19 17580904 17580905 2 + 2.559 2.602 0.145
ENSG00000130309 E025 7274.1187326 0.0041597337 3.228776e-13 8.534701e-12 19 17581177 17583162 1986 + 3.667 3.906 0.794