ENSG00000130305

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000252594 ENSG00000130305 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN5 protein_coding protein_coding 58.48082 101.8284 31.01486 5.327146 1.225449 -1.714785 30.173317 46.284656 14.967433 1.2539638 2.42425752 -1.6280552 0.5330458 0.45603333 0.48076667 0.02473333 9.237440e-01 3.629596e-11 FALSE TRUE
ENST00000310326 ENSG00000130305 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN5 protein_coding protein_coding 58.48082 101.8284 31.01486 5.327146 1.225449 -1.714785 14.238798 32.600537 6.032220 3.3861344 2.68028479 -2.4321876 0.2042250 0.31846667 0.19746667 -0.12100000 4.504079e-01 3.629596e-11 FALSE TRUE
ENST00000438747 ENSG00000130305 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN5 protein_coding protein_coding 58.48082 101.8284 31.01486 5.327146 1.225449 -1.714785 4.998629 11.876942 2.777173 0.5938088 0.07994848 -2.0925032 0.0785750 0.11670000 0.08963333 -0.02706667 3.118392e-02 3.629596e-11 FALSE TRUE
ENST00000471461 ENSG00000130305 HEK293_OSMI2_2hA HEK293_TMG_2hB NSUN5 protein_coding retained_intron 58.48082 101.8284 31.01486 5.327146 1.225449 -1.714785 6.535878 7.505709 4.814381 0.6486630 0.33212379 -0.6395655 0.1316583 0.07343333 0.15493333 0.08150000 3.629596e-11 3.629596e-11 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130305 E001 0.000000       7 73302516 73302516 1 -      
ENSG00000130305 E002 15.200946 0.0013731749 1.291162e-08 1.559296e-07 7 73302517 73303171 655 - 1.466 0.958 -1.807
ENSG00000130305 E003 6.802237 0.0034134754 2.771456e-03 9.587569e-03 7 73303172 73303224 53 - 1.076 0.695 -1.464
ENSG00000130305 E004 10.330277 0.0017477543 5.304837e-02 1.145674e-01 7 73303225 73303229 5 - 1.121 0.899 -0.817
ENSG00000130305 E005 15.462401 0.0281321757 3.779589e-01 5.222736e-01 7 73303230 73303234 5 - 1.198 1.093 -0.377
ENSG00000130305 E006 622.859989 0.0006598252 1.062048e-03 4.173707e-03 7 73303235 73303524 290 - 2.616 2.687 0.237
ENSG00000130305 E007 308.085310 0.0001490051 3.552584e-02 8.266410e-02 7 73303525 73303529 5 - 2.323 2.377 0.181
ENSG00000130305 E008 111.508856 0.0078895524 7.568352e-01 8.418695e-01 7 73303530 73303599 70 - 1.950 1.930 -0.065
ENSG00000130305 E009 683.116939 0.0003230796 1.290794e-03 4.945136e-03 7 73303600 73303740 141 - 2.664 2.724 0.202
ENSG00000130305 E010 586.755902 0.0002685290 5.358135e-04 2.293920e-03 7 73303826 73303930 105 - 2.590 2.659 0.229
ENSG00000130305 E011 531.197966 0.0001242010 1.538034e-02 4.135339e-02 7 73303931 73304036 106 - 2.565 2.612 0.158
ENSG00000130305 E012 558.238454 0.0001607986 7.145390e-01 8.107038e-01 7 73304230 73304315 86 - 2.632 2.625 -0.025
ENSG00000130305 E013 472.049518 0.0001636512 3.496915e-01 4.938942e-01 7 73304316 73304404 89 - 2.537 2.556 0.063
ENSG00000130305 E014 197.526683 0.0002402526 4.345886e-01 5.769229e-01 7 73304405 73304408 4 - 2.157 2.182 0.082
ENSG00000130305 E015 23.612074 0.0008465564 4.629389e-13 1.199595e-11 7 73304409 73304703 295 - 1.656 1.126 -1.841
ENSG00000130305 E016 573.862150 0.0001264763 2.374720e-01 3.712278e-01 7 73304747 73304862 116 - 2.620 2.641 0.072
ENSG00000130305 E017 578.109722 0.0005749409 9.858818e-01 9.951903e-01 7 73304959 73305097 139 - 2.640 2.640 0.000
ENSG00000130305 E018 557.483010 0.0001214447 2.205634e-05 1.353757e-04 7 73307394 73307502 109 - 2.689 2.610 -0.261
ENSG00000130305 E019 500.739696 0.0004380151 5.941497e-04 2.512202e-03 7 73307583 73307643 61 - 2.637 2.564 -0.242
ENSG00000130305 E020 580.477649 0.0010443766 5.512184e-02 1.182086e-01 7 73307644 73307757 114 - 2.678 2.635 -0.144
ENSG00000130305 E021 66.909986 0.0026465852 1.041119e-33 2.320435e-31 7 73307758 73308430 673 - 2.129 1.525 -2.040
ENSG00000130305 E022 448.106345 0.0016627134 5.157817e-01 6.504159e-01 7 73308431 73308553 123 - 2.514 2.534 0.067
ENSG00000130305 E023 304.996433 0.0038381503 1.097510e-01 2.051929e-01 7 73308698 73308847 150 - 2.308 2.375 0.224