ENSG00000130299

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000324894 ENSG00000130299 HEK293_OSMI2_2hA HEK293_TMG_2hB GTPBP3 protein_coding protein_coding 164.3047 234.9742 123.8189 22.69369 3.400042 -0.9242152 50.458450 86.366751 35.747786 5.3500744 1.3749309 -1.27238564 0.29578750 0.37033333 0.2897000 -0.08063333 8.104644e-02 2.168602e-06 FALSE TRUE
ENST00000594703 ENSG00000130299 HEK293_OSMI2_2hA HEK293_TMG_2hB GTPBP3 protein_coding retained_intron 164.3047 234.9742 123.8189 22.69369 3.400042 -0.9242152 10.984553 9.474093 11.404261 2.0912653 0.4151606 0.26725571 0.07525417 0.04060000 0.0922000 0.05160000 1.118930e-02 2.168602e-06   FALSE
ENST00000596001 ENSG00000130299 HEK293_OSMI2_2hA HEK293_TMG_2hB GTPBP3 protein_coding retained_intron 164.3047 234.9742 123.8189 22.69369 3.400042 -0.9242152 15.372544 19.566791 13.930125 1.9602212 0.5712194 -0.48990077 0.09731667 0.08333333 0.1124333 0.02910000 1.386674e-03 2.168602e-06 TRUE TRUE
ENST00000596166 ENSG00000130299 HEK293_OSMI2_2hA HEK293_TMG_2hB GTPBP3 protein_coding processed_transcript 164.3047 234.9742 123.8189 22.69369 3.400042 -0.9242152 32.234820 60.014956 16.494893 10.0311768 2.2239665 -1.86266869 0.17745833 0.25160000 0.1331667 -0.11843333 7.176412e-03 2.168602e-06 FALSE FALSE
ENST00000600625 ENSG00000130299 HEK293_OSMI2_2hA HEK293_TMG_2hB GTPBP3 protein_coding protein_coding 164.3047 234.9742 123.8189 22.69369 3.400042 -0.9242152 8.659744 12.329536 5.460397 0.4149007 0.0390714 -1.17357066 0.05249167 0.05320000 0.0442000 -0.00900000 3.865428e-01 2.168602e-06 FALSE TRUE
ENST00000602056 ENSG00000130299 HEK293_OSMI2_2hA HEK293_TMG_2hB GTPBP3 protein_coding retained_intron 164.3047 234.9742 123.8189 22.69369 3.400042 -0.9242152 7.650074 7.759929 7.321039 0.9712380 0.5020224 -0.08388374 0.05078333 0.03283333 0.0590000 0.02616667 2.168602e-06 2.168602e-06   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130299 E001 8.237600 3.345464e-03 1.174660e-01 2.163343e-01 19 17334920 17334999 80 + 1.032 0.845 -0.703
ENSG00000130299 E002 10.450657 2.187885e-03 8.385069e-01 8.990445e-01 19 17335000 17335111 112 + 1.010 0.986 -0.092
ENSG00000130299 E003 6.585168 7.023164e-03 9.812815e-01 9.923232e-01 19 17335112 17335122 11 + 0.823 0.818 -0.018
ENSG00000130299 E004 1.141495 3.686238e-02 3.839952e-01 5.281876e-01 19 17336686 17336691 6 + 0.180 0.349 1.259
ENSG00000130299 E005 6.539438 5.147062e-03 5.445411e-01 6.749935e-01 19 17336692 17336723 32 + 0.885 0.803 -0.317
ENSG00000130299 E006 6.761916 3.215228e-03 4.014765e-01 5.451833e-01 19 17336724 17336727 4 + 0.913 0.804 -0.423
ENSG00000130299 E007 33.569885 6.014117e-04 5.802499e-03 1.809313e-02 19 17336728 17336819 92 + 1.597 1.418 -0.613
ENSG00000130299 E008 33.068715 6.062743e-04 2.070049e-02 5.295656e-02 19 17336820 17336872 53 + 1.574 1.421 -0.522
ENSG00000130299 E009 37.479061 5.552885e-04 1.028876e-01 1.948765e-01 19 17336873 17336972 100 + 1.591 1.487 -0.354
ENSG00000130299 E010 26.116230 7.328889e-04 3.591372e-01 5.034704e-01 19 17336973 17337034 62 + 1.418 1.347 -0.243
ENSG00000130299 E011 25.012025 8.158282e-04 1.924715e-01 3.168698e-01 19 17337035 17337157 123 + 1.426 1.327 -0.345
ENSG00000130299 E012 18.720488 2.227630e-03 2.501815e-01 3.861507e-01 19 17337158 17337269 112 + 1.312 1.211 -0.355
ENSG00000130299 E013 18.081144 8.328765e-03 2.133163e-01 3.424474e-01 19 17337270 17337516 247 + 1.312 1.193 -0.418
ENSG00000130299 E014 11.703916 1.547409e-03 2.866277e-01 4.270545e-01 19 17337517 17337552 36 + 1.125 1.012 -0.408
ENSG00000130299 E015 7.513599 2.167488e-03 6.634903e-01 7.715355e-01 19 17337553 17337554 2 + 0.913 0.857 -0.216
ENSG00000130299 E016 10.759804 1.615725e-03 6.111996e-01 7.303761e-01 19 17337555 17337558 4 + 1.052 0.994 -0.211
ENSG00000130299 E017 37.438219 5.810319e-04 1.747532e-03 6.432561e-03 19 17337559 17337570 12 + 1.650 1.457 -0.658
ENSG00000130299 E018 64.278429 6.911735e-03 2.055564e-01 3.331113e-01 19 17337571 17337576 6 + 1.800 1.725 -0.254
ENSG00000130299 E019 111.174786 5.583523e-03 1.230985e-02 3.427741e-02 19 17337577 17337585 9 + 2.070 1.947 -0.414
ENSG00000130299 E020 129.568990 8.919627e-03 7.009402e-02 1.435253e-01 19 17337586 17337587 2 + 2.117 2.023 -0.315
ENSG00000130299 E021 145.303193 1.040728e-02 1.859836e-01 3.087239e-01 19 17337588 17337591 4 + 2.148 2.081 -0.225
ENSG00000130299 E022 283.384697 1.019351e-02 1.418365e-01 2.505501e-01 19 17337592 17337617 26 + 2.439 2.371 -0.225
ENSG00000130299 E023 387.528092 1.173133e-02 1.447755e-01 2.545220e-01 19 17337618 17337647 30 + 2.575 2.507 -0.226
ENSG00000130299 E024 402.064227 1.057769e-02 9.364897e-02 1.808285e-01 19 17337648 17337664 17 + 2.599 2.520 -0.262
ENSG00000130299 E025 198.429436 2.081151e-04 2.601113e-05 1.570842e-04 19 17337665 17337873 209 + 2.318 2.199 -0.397
ENSG00000130299 E026 90.851808 4.954800e-03 2.904557e-01 4.312650e-01 19 17337874 17337898 25 + 1.938 1.882 -0.188
ENSG00000130299 E027 157.113478 2.332152e-04 6.392534e-02 1.332299e-01 19 17337899 17338007 109 + 2.174 2.113 -0.204
ENSG00000130299 E028 966.387168 5.238764e-03 2.712998e-01 4.100339e-01 19 17338008 17338195 188 + 2.944 2.914 -0.100
ENSG00000130299 E029 735.696813 1.215048e-03 1.401860e-02 3.826862e-02 19 17338196 17338255 60 + 2.837 2.787 -0.165
ENSG00000130299 E030 33.482185 1.034123e-03 9.515411e-06 6.356817e-05 19 17338256 17338364 109 + 1.669 1.389 -0.959
ENSG00000130299 E031 1080.435844 4.249423e-04 1.109717e-01 2.069707e-01 19 17338365 17338451 87 + 2.983 2.960 -0.076
ENSG00000130299 E032 937.022854 1.889544e-04 1.250932e-01 2.272683e-01 19 17338539 17338571 33 + 2.920 2.897 -0.074
ENSG00000130299 E033 1935.870462 6.282461e-05 2.722240e-01 4.110798e-01 19 17338572 17338741 170 + 3.212 3.221 0.030
ENSG00000130299 E034 33.084792 5.930394e-04 1.998272e-11 3.960316e-10 19 17338742 17338743 2 + 1.736 1.323 -1.416
ENSG00000130299 E035 30.030597 6.753755e-04 3.102513e-10 5.036087e-09 19 17338758 17338759 2 + 1.693 1.287 -1.394
ENSG00000130299 E036 91.206373 4.570351e-04 5.198922e-35 1.275803e-32 19 17338760 17338953 194 + 2.196 1.723 -1.591
ENSG00000130299 E037 1454.409264 3.084481e-04 5.147990e-04 2.214761e-03 19 17338954 17339026 73 + 3.061 3.107 0.153
ENSG00000130299 E038 104.567373 6.222534e-04 2.921173e-16 1.187825e-14 19 17339027 17339122 96 + 2.165 1.860 -1.025
ENSG00000130299 E039 1380.176781 1.090412e-04 4.996689e-12 1.092954e-10 19 17339123 17339180 58 + 3.011 3.091 0.266
ENSG00000130299 E040 1467.930163 1.714944e-04 4.924541e-17 2.195152e-15 19 17339181 17339266 86 + 3.022 3.123 0.335
ENSG00000130299 E041 61.052797 4.718228e-04 5.485301e-14 1.618651e-12 19 17339389 17339433 45 + 1.961 1.609 -1.189
ENSG00000130299 E042 1603.377847 4.291459e-04 2.701257e-07 2.522776e-06 19 17339434 17339599 166 + 3.084 3.156 0.239
ENSG00000130299 E043 22.744284 9.040707e-04 6.857339e-15 2.318199e-13 19 17340946 17341043 98 + 1.655 1.092 -1.960
ENSG00000130299 E044 360.158885 2.588468e-04 7.279151e-01 8.204692e-01 19 17341044 17341106 63 + 2.496 2.487 -0.031
ENSG00000130299 E045 739.153683 1.926211e-03 3.619924e-01 5.063975e-01 19 17341107 17341320 214 + 2.820 2.799 -0.069
ENSG00000130299 E046 254.679998 2.152789e-03 9.833985e-01 9.936856e-01 19 17341321 17341322 2 + 2.341 2.342 0.004
ENSG00000130299 E047 4.956149 1.109696e-02 2.462067e-03 8.649949e-03 19 17341323 17341325 3 + 0.988 0.567 -1.700
ENSG00000130299 E048 892.829337 9.771251e-04 5.475216e-01 6.775757e-01 19 17341478 17341733 256 + 2.894 2.884 -0.035
ENSG00000130299 E049 1096.840569 2.367683e-03 3.293526e-01 4.728567e-01 19 17341734 17342155 422 + 2.956 2.984 0.093
ENSG00000130299 E050 1047.382540 8.189675e-03 8.350916e-02 1.650451e-01 19 17342156 17342731 576 + 2.896 2.976 0.267