ENSG00000130147

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000392011 ENSG00000130147 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3BP4 protein_coding protein_coding 7.532821 8.66237 6.736982 0.5574667 0.1620477 -0.362184 6.8567216 7.1703361 6.4537093 1.00108405 0.10194885 -0.151689 0.91434167 0.82106667 0.95836667 0.13730000 0.067780473 0.000606006 FALSE  
ENST00000484097 ENSG00000130147 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3BP4 protein_coding processed_transcript 7.532821 8.66237 6.736982 0.5574667 0.1620477 -0.362184 0.3039479 0.6462394 0.1210871 0.07883963 0.03083864 -2.323696 0.03689583 0.07433333 0.01780000 -0.05653333 0.000606006 0.000606006    
MSTRG.20103.3 ENSG00000130147 HEK293_OSMI2_2hA HEK293_TMG_2hB SH3BP4 protein_coding   7.532821 8.66237 6.736982 0.5574667 0.1620477 -0.362184 0.2213416 0.7045166 0.1183402 0.57909577 0.03775950 -2.476994 0.02960000 0.08716667 0.01766667 -0.06950000 0.897779182 0.000606006 FALSE  
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000130147 E001 2.6583348 0.005886613 6.527736e-03 1.999450e-02 2 234952017 234952034 18 + 0.736 0.350 -1.844
ENSG00000130147 E002 3.0270290 0.005149538 8.791215e-03 2.576212e-02 2 234952035 234952041 7 + 0.763 0.406 -1.628
ENSG00000130147 E003 5.5242965 0.003188180 1.040956e-03 4.100683e-03 2 234952042 234952075 34 + 0.980 0.612 -1.465
ENSG00000130147 E004 15.7314535 0.025141638 6.104932e-02 1.284012e-01 2 234952076 234952170 95 + 1.289 1.150 -0.490
ENSG00000130147 E005 4.8146943 0.003613326 1.492426e-03 5.611617e-03 2 234952715 234953190 476 + 0.374 0.910 2.381
ENSG00000130147 E006 0.0000000       2 234978685 234978699 15 +      
ENSG00000130147 E007 0.0000000       2 234978700 234978702 3 +      
ENSG00000130147 E008 0.0000000       2 234978703 234978704 2 +      
ENSG00000130147 E009 0.0000000       2 234978705 234978900 196 +      
ENSG00000130147 E010 0.0000000       2 234978901 234978985 85 +      
ENSG00000130147 E011 0.6643356 0.867411198 4.214046e-01 5.645598e-01 2 234979462 234979898 437 + 0.000 0.308 10.184
ENSG00000130147 E012 0.0000000       2 234981624 234981633 10 +      
ENSG00000130147 E013 0.0000000       2 234981634 234981727 94 +      
ENSG00000130147 E014 0.0000000       2 234983243 234983296 54 +      
ENSG00000130147 E015 0.0000000       2 234994524 234994628 105 +      
ENSG00000130147 E016 0.0000000       2 234994840 234994960 121 +      
ENSG00000130147 E017 18.9141256 0.017792287 3.232316e-03 1.095090e-02 2 234995303 234995376 74 + 1.403 1.179 -0.785
ENSG00000130147 E018 0.2955422 0.028873598 8.501022e-01   2 234995692 234995993 302 + 0.128 0.117 -0.146
ENSG00000130147 E019 0.0000000       2 235008560 235008594 35 +      
ENSG00000130147 E020 26.9514007 0.004569445 1.866158e-03 6.809304e-03 2 235034871 235034954 84 + 1.520 1.363 -0.544
ENSG00000130147 E021 45.6678819 0.003121606 1.915559e-03 6.963920e-03 2 235034955 235035120 166 + 1.719 1.610 -0.368
ENSG00000130147 E022 58.6635772 0.003153855 7.456639e-05 4.021058e-04 2 235040888 235041037 150 + 1.839 1.702 -0.463
ENSG00000130147 E023 86.8508116 0.002092571 1.309401e-08 1.579711e-07 2 235041038 235041361 324 + 2.024 1.854 -0.570
ENSG00000130147 E024 356.7311387 0.001045107 5.853023e-03 1.822577e-02 2 235041362 235043247 1886 + 2.540 2.556 0.053
ENSG00000130147 E025 82.2943998 0.002133807 9.970481e-01 1.000000e+00 2 235052562 235052750 189 + 1.878 1.947 0.231
ENSG00000130147 E026 528.3013351 0.006621673 4.070442e-09 5.389147e-08 2 235053592 235055714 2123 + 2.576 2.803 0.754