ENSG00000129933

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000262815 ENSG00000129933 HEK293_OSMI2_2hA HEK293_TMG_2hB MAU2 protein_coding protein_coding 30.07098 33.51226 24.72293 2.009537 0.4820913 -0.4386864 12.262413 13.780680 11.8258724 0.7747170 0.1872260 -0.2205276 0.41267917 0.41170000 0.47840000 0.066700000 4.937568e-02 2.743887e-07 FALSE TRUE
ENST00000587638 ENSG00000129933 HEK293_OSMI2_2hA HEK293_TMG_2hB MAU2 protein_coding retained_intron 30.07098 33.51226 24.72293 2.009537 0.4820913 -0.4386864 2.935717 3.158747 2.2010262 0.6675425 0.1676182 -0.5191966 0.09688750 0.09303333 0.08886667 -0.004166667 9.832013e-01 2.743887e-07 FALSE FALSE
ENST00000589637 ENSG00000129933 HEK293_OSMI2_2hA HEK293_TMG_2hB MAU2 protein_coding retained_intron 30.07098 33.51226 24.72293 2.009537 0.4820913 -0.4386864 3.091116 8.185373 0.8977207 1.0144391 0.1646965 -3.1744892 0.09752083 0.24920000 0.03643333 -0.212766667 2.743887e-07 2.743887e-07 FALSE TRUE
MSTRG.16763.1 ENSG00000129933 HEK293_OSMI2_2hA HEK293_TMG_2hB MAU2 protein_coding   30.07098 33.51226 24.72293 2.009537 0.4820913 -0.4386864 5.227191 4.436455 3.5233989 1.0607197 0.4652481 -0.3315988 0.17366667 0.12966667 0.14196667 0.012300000 8.877189e-01 2.743887e-07 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000129933 E001 3.8300319 0.0072753509 2.362615e-01 3.698422e-01 19 19320829 19320853 25 + 0.753 0.628 -0.519
ENSG00000129933 E002 73.7581668 0.0003526155 2.976700e-09 4.040748e-08 19 19320854 19320985 132 + 1.964 1.779 -0.622
ENSG00000129933 E003 112.9948519 0.0002547141 1.705976e-10 2.893134e-09 19 19320986 19321135 150 + 2.130 1.977 -0.515
ENSG00000129933 E004 2.6800015 0.0096250903 5.217241e-01 6.556137e-01 19 19321525 19321903 379 + 0.587 0.527 -0.275
ENSG00000129933 E005 1.5071559 0.2147164613 8.819473e-01 9.283586e-01 19 19334173 19334602 430 + 0.317 0.407 0.533
ENSG00000129933 E006 52.7678010 0.0004275016 1.281320e-04 6.506613e-04 19 19335718 19335735 18 + 1.789 1.662 -0.430
ENSG00000129933 E007 76.5682363 0.0023311785 6.019334e-04 2.540366e-03 19 19336122 19336187 66 + 1.934 1.836 -0.329
ENSG00000129933 E008 77.3220747 0.0083920703 1.337334e-02 3.678805e-02 19 19337170 19337205 36 + 1.934 1.848 -0.288
ENSG00000129933 E009 98.5967471 0.0020927179 4.225160e-03 1.377179e-02 19 19337206 19337265 60 + 2.021 1.963 -0.195
ENSG00000129933 E010 124.9815846 0.0006068738 9.347584e-03 2.715103e-02 19 19338845 19338939 95 + 2.104 2.077 -0.091
ENSG00000129933 E011 0.2965864 0.2950039940 3.868565e-01   19 19339667 19339704 38 + 0.000 0.197 10.195
ENSG00000129933 E012 75.7804518 0.0014445700 1.034093e-02 2.960690e-02 19 19340846 19340873 28 + 1.904 1.851 -0.180
ENSG00000129933 E013 0.1482932 0.0420591712 6.538885e-01   19 19340874 19340876 3 + 0.000 0.112 10.033
ENSG00000129933 E014 138.5442106 0.0035220351 5.423316e-01 6.731116e-01 19 19341252 19341407 156 + 2.113 2.148 0.120
ENSG00000129933 E015 115.1693884 0.0018664174 1.855556e-01 3.081997e-01 19 19342535 19342617 83 + 2.050 2.058 0.027
ENSG00000129933 E016 119.2682231 0.0002891342 2.741112e-02 6.682565e-02 19 19342618 19342681 64 + 2.075 2.061 -0.046
ENSG00000129933 E017 136.2364830 0.0002459276 7.952491e-03 2.365754e-02 19 19342776 19342866 91 + 2.138 2.116 -0.076
ENSG00000129933 E018 0.0000000       19 19342867 19342875 9 +      
ENSG00000129933 E019 136.3346185 0.0002464889 7.313205e-03 2.203312e-02 19 19343837 19343940 104 + 2.142 2.119 -0.077
ENSG00000129933 E020 3.9365569 0.0041973575 4.538541e-02 1.009022e-01 19 19343941 19344159 219 + 0.804 0.564 -1.013
ENSG00000129933 E021 8.8657666 0.0019077619 5.244264e-04 2.250907e-03 19 19344252 19344848 597 + 1.143 0.821 -1.199
ENSG00000129933 E022 121.9445229 0.0002898235 2.765111e-02 6.731545e-02 19 19344849 19344926 78 + 2.084 2.071 -0.043
ENSG00000129933 E023 12.1604992 0.0017627966 9.047153e-04 3.630570e-03 19 19344927 19345303 377 + 1.251 0.994 -0.926
ENSG00000129933 E024 127.2931674 0.0013349744 5.694933e-03 1.780343e-02 19 19345304 19345369 66 + 2.117 2.079 -0.128
ENSG00000129933 E025 16.5186994 0.0014473939 1.459923e-06 1.170800e-05 19 19346877 19347018 142 + 1.410 1.066 -1.217
ENSG00000129933 E026 23.1421168 0.0008043378 2.715370e-03 9.419172e-03 19 19347019 19347279 261 + 1.456 1.299 -0.544
ENSG00000129933 E027 165.4924158 0.0004011015 1.815290e-04 8.845423e-04 19 19347280 19347363 84 + 2.236 2.188 -0.159
ENSG00000129933 E028 69.5114070 0.0005426977 2.667446e-02 6.531382e-02 19 19347364 19347366 3 + 1.857 1.820 -0.124
ENSG00000129933 E029 6.0606283 0.1268488002 2.064216e-02 5.283089e-02 19 19347367 19347370 4 + 1.043 0.604 -1.736
ENSG00000129933 E030 6.0562863 0.1405774938 4.940786e-02 1.081070e-01 19 19347371 19347389 19 + 1.014 0.661 -1.381
ENSG00000129933 E031 33.4503485 0.0005845120 3.213096e-06 2.388227e-05 19 19347650 19348714 1065 + 1.643 1.422 -0.758
ENSG00000129933 E032 12.9353697 0.0013848927 3.774106e-01 5.217328e-01 19 19348715 19348888 174 + 1.154 1.128 -0.095
ENSG00000129933 E033 100.4646057 0.0002888769 1.330841e-01 2.384240e-01 19 19348889 19348897 9 + 1.990 1.995 0.017
ENSG00000129933 E034 149.5520657 0.0003435959 5.036285e-01 6.395947e-01 19 19348898 19348938 41 + 2.141 2.180 0.132
ENSG00000129933 E035 7.1944203 0.0022960638 6.819380e-03 2.075259e-02 19 19348939 19349154 216 + 1.043 0.780 -0.997
ENSG00000129933 E036 188.1986011 0.0002748643 3.196441e-01 4.627441e-01 19 19349155 19349232 78 + 2.243 2.276 0.110
ENSG00000129933 E037 220.0393587 0.0002056118 2.159304e-01 3.455247e-01 19 19349325 19349436 112 + 2.314 2.343 0.097
ENSG00000129933 E038 2.5364541 0.5012983295 8.414504e-01 9.011167e-01 19 19352270 19352395 126 + 0.592 0.496 -0.447
ENSG00000129933 E039 14.9598670 0.0013183892 4.294061e-01 5.721277e-01 19 19352398 19354203 1806 + 1.196 1.182 -0.049
ENSG00000129933 E040 174.4012169 0.0010241861 1.654186e-01 2.822410e-01 19 19354355 19354445 91 + 2.166 2.269 0.346
ENSG00000129933 E041 8.2262286 0.0040305193 1.618355e-01 2.775246e-01 19 19354446 19354610 165 + 1.014 0.908 -0.392
ENSG00000129933 E042 6.4115629 0.1909214366 2.557582e-01 3.926850e-01 19 19355143 19355263 121 + 0.965 0.798 -0.639
ENSG00000129933 E043 213.2520124 0.0003651230 2.319553e-02 5.815598e-02 19 19355264 19355391 128 + 2.242 2.362 0.397
ENSG00000129933 E044 2301.5172534 0.0027187650 7.473334e-12 1.587755e-10 19 19355708 19358754 3047 + 3.241 3.417 0.587