ENSG00000129757

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000380725 ENSG00000129757 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKN1C protein_coding protein_coding 2.663601 3.79261 2.664913 0.27372 0.3289664 -0.5074983 0.8593700 1.93797922 0.1899323 0.15787813 0.18993229 -3.2843949 0.35183333 0.51300000 0.0804000 -0.43260000 0.049514742 0.000561294 FALSE FALSE
ENST00000430149 ENSG00000129757 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKN1C protein_coding protein_coding 2.663601 3.79261 2.664913 0.27372 0.3289664 -0.5074983 0.1364223 0.04747193 0.1070975 0.04747193 0.05092336 1.0267807 0.05091667 0.01446667 0.0434000 0.02893333 0.489097763 0.000561294 FALSE TRUE
ENST00000440480 ENSG00000129757 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKN1C protein_coding protein_coding 2.663601 3.79261 2.664913 0.27372 0.3289664 -0.5074983 1.1376393 1.39065151 1.6141687 0.08298518 0.11415227 0.2136034 0.41287500 0.36753333 0.6130667 0.24553333 0.000561294 0.000561294 FALSE TRUE
ENST00000647251 ENSG00000129757 HEK293_OSMI2_2hA HEK293_TMG_2hB CDKN1C protein_coding protein_coding 2.663601 3.79261 2.664913 0.27372 0.3289664 -0.5074983 0.4232002 0.17247450 0.7152607 0.17247450 0.39240548 1.9908047 0.14646667 0.04446667 0.2501667 0.20570000 0.481174168 0.000561294 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000129757 E001 0.1817044 0.0396671847 0.7517426213   11 2883213 2883217 5 - 0.000 0.099 7.939
ENSG00000129757 E002 3.7147443 0.0172671820 0.4080782223 0.55165980 11 2883218 2883239 22 - 0.590 0.725 0.576
ENSG00000129757 E003 32.6524814 0.0007384016 0.0005222177 0.00224281 11 2883240 2883615 376 - 1.352 1.570 0.749
ENSG00000129757 E004 6.5464305 0.0029598330 0.0310324055 0.07397941 11 2883616 2883631 16 - 0.634 0.926 1.170
ENSG00000129757 E005 9.1606512 0.0022989656 0.0081753289 0.02422674 11 2883632 2883674 43 - 0.744 1.061 1.210
ENSG00000129757 E006 27.8233757 0.0147918866 0.7491135120 0.83614552 11 2883675 2883910 236 - 1.425 1.453 0.096
ENSG00000129757 E007 10.5465215 0.0018744100 0.0265321032 0.06501708 11 2883911 2883915 5 - 1.178 0.964 -0.778
ENSG00000129757 E008 23.2957671 0.0009994638 0.1505316211 0.26240288 11 2883999 2884134 136 - 1.432 1.337 -0.328
ENSG00000129757 E009 0.8407440 0.0147023962 0.3317681646 0.47540246 11 2884135 2884669 535 - 0.350 0.180 -1.273
ENSG00000129757 E010 22.1566987 0.0010383838 0.0191971342 0.04976518 11 2884670 2885201 532 - 1.439 1.277 -0.560
ENSG00000129757 E011 46.9779113 0.0006768280 0.1871949199 0.31021541 11 2885202 2885466 265 - 1.693 1.634 -0.202
ENSG00000129757 E012 2.2381407 0.0507232282 0.9790534799 0.99094289 11 2885467 2885633 167 - 0.487 0.486 -0.002
ENSG00000129757 E013 31.9052637 0.0006678695 0.8576370421 0.91207857 11 2885634 2885775 142 - 1.499 1.491 -0.029