ENSG00000129472

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000397762 ENSG00000129472 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB2B protein_coding protein_coding 11.46177 9.315355 14.84701 1.083353 1.224612 0.6719137 7.9023898 7.382636 8.6108810 0.7883669 0.2673710 0.2217464 0.7148792 0.7948333 0.5845667 -0.2102667 6.162783e-05 6.162783e-05 FALSE TRUE
ENST00000461909 ENSG00000129472 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB2B protein_coding processed_transcript 11.46177 9.315355 14.84701 1.083353 1.224612 0.6719137 3.0599160 1.901624 4.9104346 0.3242742 1.4682724 1.3639871 0.2468417 0.2017000 0.3216333 0.1199333 4.465688e-01 6.162783e-05 FALSE FALSE
ENST00000649801 ENSG00000129472 HEK293_OSMI2_2hA HEK293_TMG_2hB RAB2B protein_coding protein_coding 11.46177 9.315355 14.84701 1.083353 1.224612 0.6719137 0.1539067 0.000000 0.7640634 0.0000000 0.7640634 6.2743797 0.0118000 0.0000000 0.0557000 0.0557000 9.154559e-01 6.162783e-05 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000129472 E001 178.722073 0.0086821663 2.409345e-21 1.800564e-19 14 21459020 21459667 648 - 1.958 2.457 1.667
ENSG00000129472 E002 200.249512 0.0013019659 1.307935e-04 6.622593e-04 14 21459668 21460340 673 - 2.232 2.382 0.501
ENSG00000129472 E003 148.318776 0.0003326481 1.421348e-07 1.402595e-06 14 21460341 21461197 857 - 2.219 2.120 -0.329
ENSG00000129472 E004 55.755379 0.0004245516 6.662717e-02 1.377757e-01 14 21461198 21461238 41 - 1.774 1.739 -0.117
ENSG00000129472 E005 43.220839 0.0005315538 1.584431e-02 4.238851e-02 14 21461239 21461244 6 - 1.683 1.606 -0.262
ENSG00000129472 E006 59.434000 0.0004017023 2.801750e-02 6.805085e-02 14 21461245 21461303 59 - 1.808 1.760 -0.162
ENSG00000129472 E007 62.895470 0.0007920887 1.563954e-01 2.703278e-01 14 21462350 21462418 69 - 1.817 1.804 -0.043
ENSG00000129472 E008 77.115256 0.0003494033 4.422947e-02 9.881062e-02 14 21463656 21463767 112 - 1.909 1.880 -0.097
ENSG00000129472 E009 66.867037 0.0006204636 2.791880e-03 9.649345e-03 14 21468357 21468449 93 - 1.868 1.788 -0.268
ENSG00000129472 E010 69.435113 0.0006366611 1.031988e-05 6.840215e-05 14 21468670 21468752 83 - 1.908 1.769 -0.468
ENSG00000129472 E011 2.277987 0.0101600594 1.248108e-02 3.469047e-02 14 21474520 21474866 347 - 0.656 0.251 -2.175
ENSG00000129472 E012 63.142464 0.0004459144 1.329395e-04 6.718508e-04 14 21474867 21474934 68 - 1.858 1.739 -0.402
ENSG00000129472 E013 0.000000       14 21476267 21476527 261 -      
ENSG00000129472 E014 60.082046 0.0004150824 6.468962e-04 2.705056e-03 14 21476528 21476599 72 - 1.833 1.729 -0.349
ENSG00000129472 E015 10.366608 0.0126027240 9.716562e-01 9.862801e-01 14 21476600 21476673 74 - 1.043 1.088 0.161
ENSG00000129472 E016 30.086340 0.0060863288 2.704105e-03 9.386282e-03 14 21476827 21476960 134 - 1.563 1.387 -0.603