ENSG00000129315

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000261900 ENSG00000129315 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT1 protein_coding protein_coding 13.77586 4.54999 23.74681 1.020356 0.1754704 2.381239 11.515369 2.578191 20.585472 0.5468355 0.07539544 2.9923113 0.7820708 0.5721667 0.86693333 0.2947667 1.435612e-17 1.435612e-17 FALSE TRUE
ENST00000618666 ENSG00000129315 HEK293_OSMI2_2hA HEK293_TMG_2hB CCNT1 protein_coding protein_coding 13.77586 4.54999 23.74681 1.020356 0.1754704 2.381239 1.737771 1.885147 1.747103 0.4742397 0.87819666 -0.1091114 0.1832708 0.4087333 0.07336667 -0.3353667 2.158005e-01 1.435612e-17 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000129315 E001 1620.0407059 0.0233609523 3.444634e-11 6.565147e-10 12 48688458 48693032 4575 - 2.906 3.408 1.670
ENSG00000129315 E002 304.1579634 0.0231677554 3.799034e-04 1.696962e-03 12 48693033 48693929 897 - 2.264 2.310 0.155
ENSG00000129315 E003 219.6329562 0.0002072582 8.400554e-25 9.042385e-23 12 48693930 48694436 507 - 2.132 2.110 -0.072
ENSG00000129315 E004 87.0187312 0.0003816030 1.778063e-10 3.006165e-09 12 48695759 48695829 71 - 1.735 1.714 -0.072
ENSG00000129315 E005 2.7271212 0.0055138677 9.270794e-01 9.581117e-01 12 48695830 48695977 148 - 0.398 0.620 1.079
ENSG00000129315 E006 100.1241423 0.0016903647 1.639284e-12 3.876154e-11 12 48695999 48696162 164 - 1.800 1.731 -0.233
ENSG00000129315 E007 0.6235652 0.0255386429 6.908737e-01 7.924436e-01 12 48697953 48697962 10 - 0.114 0.313 1.817
ENSG00000129315 E008 56.1913191 0.0004315282 3.230383e-06 2.400040e-05 12 48698138 48698183 46 - 1.547 1.568 0.071
ENSG00000129315 E009 55.9987829 0.0004757047 5.827568e-08 6.190856e-07 12 48699778 48699840 63 - 1.551 1.501 -0.171
ENSG00000129315 E010 63.5335689 0.0100128906 3.771552e-05 2.187719e-04 12 48701013 48701073 61 - 1.601 1.590 -0.037
ENSG00000129315 E011 0.0000000       12 48705624 48705767 144 -      
ENSG00000129315 E012 83.2903660 0.0003476080 5.335005e-09 6.925454e-08 12 48705768 48705896 129 - 1.714 1.723 0.030
ENSG00000129315 E013 75.0848685 0.0003248286 1.590338e-12 3.767472e-11 12 48714443 48714524 82 - 1.681 1.568 -0.385
ENSG00000129315 E014 85.2315295 0.0025471718 5.189401e-18 2.626547e-16 12 48716515 48716998 484 - 1.745 1.455 -0.991