ENSG00000129244

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000250111 ENSG00000129244 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP1B2 protein_coding protein_coding 2.013932 3.0349 1.278093 0.06325919 0.06894897 -1.241158 1.7088337 1.9182809 1.278093 0.4050984 0.06894897 -0.5820782 0.8866542 0.6290000 1 0.3710000 0.0002754583 0.0002754583 FALSE TRUE
ENST00000577026 ENSG00000129244 HEK293_OSMI2_2hA HEK293_TMG_2hB ATP1B2 protein_coding protein_coding 2.013932 3.0349 1.278093 0.06325919 0.06894897 -1.241158 0.2891499 0.9890326 0.000000 0.4948787 0.00000000 -6.6424598 0.1082167 0.3299667 0 -0.3299667 0.2341169774 0.0002754583 FALSE TRUE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000129244 E001 0.0000000       17 7646627 7646701 75 +      
ENSG00000129244 E002 25.5421657 0.0009020825 0.003536349 0.01183017 17 7650926 7651630 705 + 1.495 1.296 -0.688
ENSG00000129244 E003 17.6575254 0.0011770121 0.856516421 0.91126793 17 7653374 7653502 129 + 1.215 1.200 -0.051
ENSG00000129244 E004 20.0855376 0.0019679664 0.715906585 0.81169642 17 7653841 7653945 105 + 1.278 1.249 -0.102
ENSG00000129244 E005 17.5977017 0.0010786075 0.227484790 0.35940248 17 7654052 7654108 57 + 1.278 1.177 -0.356
ENSG00000129244 E006 27.9323312 0.0008091666 0.341353701 0.48525203 17 7654109 7654257 149 + 1.442 1.379 -0.220
ENSG00000129244 E007 17.8909877 0.0010270724 0.694607648 0.79539256 17 7654628 7654684 57 + 1.187 1.222 0.126
ENSG00000129244 E008 0.2214452 0.0375778935 1.000000000   17 7654792 7654818 27 + 0.001 0.082 7.333
ENSG00000129244 E009 0.0000000       17 7655236 7655256 21 +      
ENSG00000129244 E010 17.5724428 0.0072529974 0.582532566 0.70687697 17 7655527 7655625 99 + 1.172 1.227 0.194
ENSG00000129244 E011 127.1092625 0.0005018202 0.007197319 0.02173812 17 7655731 7657770 2040 + 1.987 2.061 0.250