ENSG00000128294

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000338754 ENSG00000128294 HEK293_OSMI2_2hA HEK293_TMG_2hB TPST2 protein_coding protein_coding 27.33133 37.90209 24.02768 1.336024 0.3080252 -0.6573606 2.938053 2.675302 4.040611 0.3805715 0.1503368 0.5930552 0.1134625 0.0709000 0.1681333 0.097233333 9.840499e-05 9.840499e-05 FALSE TRUE
ENST00000398110 ENSG00000128294 HEK293_OSMI2_2hA HEK293_TMG_2hB TPST2 protein_coding protein_coding 27.33133 37.90209 24.02768 1.336024 0.3080252 -0.6573606 11.486122 14.886654 9.006635 0.7769116 0.6400490 -0.7243274 0.4232417 0.3923667 0.3746333 -0.017733333 8.518048e-01 9.840499e-05 FALSE TRUE
MSTRG.21854.4 ENSG00000128294 HEK293_OSMI2_2hA HEK293_TMG_2hB TPST2 protein_coding   27.33133 37.90209 24.02768 1.336024 0.3080252 -0.6573606 7.588205 11.451869 5.393405 1.8681825 0.2751468 -1.0849017 0.2713292 0.2996333 0.2242333 -0.075400000 3.300486e-01 9.840499e-05 TRUE TRUE
MSTRG.21854.5 ENSG00000128294 HEK293_OSMI2_2hA HEK293_TMG_2hB TPST2 protein_coding   27.33133 37.90209 24.02768 1.336024 0.3080252 -0.6573606 4.606639 7.730342 4.874367 1.2430412 0.8079868 -0.6642256 0.1657583 0.2066333 0.2031667 -0.003466667 1.000000e+00 9.840499e-05 FALSE FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000128294 E001 292.0757371 0.0187476477 1.451994e-07 1.429634e-06 22 26521996 26525745 3750 - 2.649 2.287 -1.205
ENSG00000128294 E002 41.6907374 0.0005296427 4.560024e-01 5.964806e-01 22 26525746 26525823 78 - 1.649 1.580 -0.235
ENSG00000128294 E003 73.0897862 0.0012743042 1.324820e-03 5.058922e-03 22 26525824 26525894 71 - 1.757 1.879 0.414
ENSG00000128294 E004 500.4504800 0.0004004497 9.423685e-07 7.861592e-06 22 26525895 26526267 373 - 2.631 2.689 0.192
ENSG00000128294 E005 241.6679003 0.0026485779 1.792068e-01 3.001414e-01 22 26528214 26528262 49 - 2.346 2.360 0.048
ENSG00000128294 E006 229.4485442 0.0004658945 4.452870e-01 5.868484e-01 22 26532695 26532745 51 - 2.340 2.331 -0.031
ENSG00000128294 E007 3.7394493 0.0938957027 2.872461e-01 4.277365e-01 22 26535895 26536287 393 - 0.791 0.567 -0.947
ENSG00000128294 E008 300.7464237 0.0001548468 7.458246e-01 8.337481e-01 22 26536288 26536486 199 - 2.474 2.438 -0.118
ENSG00000128294 E009 243.7799659 0.0030950622 3.384165e-01 4.822844e-01 22 26540789 26540949 161 - 2.400 2.337 -0.209
ENSG00000128294 E010 301.0526521 0.0001796042 3.883893e-01 5.324619e-01 22 26540950 26541205 256 - 2.483 2.436 -0.155
ENSG00000128294 E011 123.4695915 0.0002398755 3.730252e-01 5.174678e-01 22 26541206 26541270 65 - 2.065 2.066 0.004
ENSG00000128294 E012 151.6605778 0.0019354937 9.447196e-02 1.820896e-01 22 26541271 26541438 168 - 2.131 2.163 0.106
ENSG00000128294 E013 109.6278436 0.0017887892 1.698987e-01 2.880818e-01 22 26541439 26541484 46 - 1.994 2.019 0.086
ENSG00000128294 E014 82.5940210 0.0003495842 1.797815e-02 4.708799e-02 22 26541485 26541489 5 - 1.843 1.912 0.234
ENSG00000128294 E015 221.5936311 0.0031848143 2.121744e-02 5.404913e-02 22 26541490 26541718 229 - 2.279 2.333 0.182
ENSG00000128294 E016 1.7326917 0.0083001731 4.855938e-02 1.066081e-01 22 26543108 26543277 170 - 0.156 0.506 2.354
ENSG00000128294 E017 143.6556358 0.0033212695 2.092617e-01 3.376372e-01 22 26544604 26544675 72 - 2.113 2.135 0.076
ENSG00000128294 E018 0.8783198 0.0144697168 3.964838e-01 5.402503e-01 22 26547500 26547618 119 - 0.156 0.297 1.186
ENSG00000128294 E019 0.1515154 0.0426188968 3.039740e-01   22 26550592 26550653 62 - 0.156 0.000 -11.104
ENSG00000128294 E020 0.1482932 0.0412190861 7.583794e-01   22 26565224 26565368 145 - 0.000 0.095 8.970
ENSG00000128294 E021 0.9952782 0.0125792312 2.637788e-01 4.016985e-01 22 26565447 26565726 280 - 0.156 0.347 1.505
ENSG00000128294 E022 109.3890020 0.0028884063 5.032197e-01 6.391954e-01 22 26590053 26590154 102 - 2.011 2.011 0.000
ENSG00000128294 E023 0.0000000       22 26596627 26596717 91 -