ENSG00000127928

1 Isoform Switch

Summary Plot

Only the isoforms with fraction > 5% are shown below.

Notes on IDR and IDR_w_binding: As the prediction of Intrinsically Disordered Regions (IDR) and Intrinsically Disordered Binding Regions (IDBR) are done by two different tools we require two things to annotate the Intrinsically Disordered Binding Regions (IDBR). Firstly the IDBR (predicted by ANCHOR2) must be a region of at least 15 amino acids (after the smoothing). Secondly the fraction of the IDBR which overlaps the IDR predictions (done by IUPred3, again after smoothing) must be at least 80%. When that is the case the IDR type will be annotated as “IDR_w_binding_region” instead of just “IDR”. The current default parameters have not been rigorously tested and should be considered experimental.

For the function of any other domain, please Click HERE to search it through Pfam database.

Results

isoform_id gene_id condition_1 condition_2 gene_name gene_biotype iso_biotype gene_overall_mean gene_value_1 gene_value_2 gene_stderr_1 gene_stderr_2 gene_log2_fold_change iso_overall_mean iso_value_1 iso_value_2 iso_stderr_1 iso_stderr_2 iso_log2_fold_change IF_overall IF1 IF2 dIF isoform_switch_q_value gene_switch_q_value PTC codingPotential
ENST00000429473 ENSG00000127928 HEK293_OSMI2_2hA HEK293_TMG_2hB GNGT1 protein_coding protein_coding 4.191634 1.639547 6.311027 0.06334389 0.2174955 1.938089 0.1637337 0.09655835 0.0000000 0.05201801 0.0000000 -3.4135717 0.05294167 0.06126667 0.00000000 -0.06126667 0.046994483 0.000217552   FALSE
MSTRG.30228.1 ENSG00000127928 HEK293_OSMI2_2hA HEK293_TMG_2hB GNGT1 protein_coding   4.191634 1.639547 6.311027 0.06334389 0.2174955 1.938089 3.3909773 1.08922634 5.5576862 0.09650457 0.1944492 2.3405894 0.74894583 0.66336667 0.88066667 0.21730000 0.000217552 0.000217552   FALSE
MSTRG.30228.4 ENSG00000127928 HEK293_OSMI2_2hA HEK293_TMG_2hB GNGT1 protein_coding   4.191634 1.639547 6.311027 0.06334389 0.2174955 1.938089 0.3913764 0.33162381 0.4522061 0.05392023 0.1530747 0.4361278 0.13363333 0.20053333 0.07173333 -0.12880000 0.052436563 0.000217552   FALSE
  • Note:
    • The comparisons made can be identified as “from ‘condition_1’ to ‘condition_2’”, meaning ‘condition_1’ is considered the ground state and ‘condition_2’ the changed state. This also means that a positive dIF value indicates that the isoform usage is increased in ‘condition_2’ compared to ‘condition_1’. Since the ‘isoformFeatures’ entry is the most relevant part of the switchAnalyzeRlist object, the most-used standard methods have also been implemented to work directly on isoformFeatures.

2 Differential Exon Usage

Counts

All exons whithin this gene region are shown and numbering below.

Expression

All exons whithin this gene region are shown and numbering below.

Splicing

All exons whithin this gene region are shown and numbering below.

Transcripts

All isoforms whithin this gene region are shown below.

Results

groupID featureID exonBaseMean dispersion pvalue padj seqnames start end width strand HEK293_TMG_2hB HEK293_OSMI2_2hA log2fold_HEK293_OSMI2_2hA_HEK293_TMG_2hB
ENSG00000127928 E001 2.2863046 0.0070937468 7.563890e-01 8.414736e-01 7 93591322 93591348 27 + 0.464 0.536 0.349
ENSG00000127928 E002 13.6907260 0.0014601034 6.238986e-01 7.403705e-01 7 93591349 93591425 77 + 1.125 1.099 -0.093
ENSG00000127928 E003 19.1619021 0.0010019363 6.173894e-01 7.352127e-01 7 93591426 93591510 85 + 1.235 1.298 0.224
ENSG00000127928 E004 12.5159149 0.0018401567 8.882098e-01 9.325413e-01 7 93591511 93591522 12 + 1.071 1.078 0.026
ENSG00000127928 E005 16.2081003 0.0013508828 7.896420e-01 8.653930e-01 7 93591523 93591572 50 + 1.178 1.177 -0.007
ENSG00000127928 E006 17.5991321 0.0011776640 7.349533e-01 8.257992e-01 7 93591573 93591656 84 + 1.217 1.210 -0.022
ENSG00000127928 E007 21.2927196 0.0008837915 1.734835e-01 2.927238e-01 7 93591657 93591771 115 + 1.315 1.226 -0.310
ENSG00000127928 E008 31.4323885 0.0006926076 3.179050e-07 2.926156e-06 7 93807204 93807585 382 + 1.523 1.158 -1.270
ENSG00000127928 E009 3.0937486 0.0085192147 2.257993e-02 5.687977e-02 7 93886506 93886549 44 + 0.437 0.812 1.658
ENSG00000127928 E010 0.1451727 0.0435840348 1.000000e+00   7 93906567 93906585 19 + 0.070 0.000 -10.520
ENSG00000127928 E011 0.1451727 0.0435840348 1.000000e+00   7 93906586 93906591 6 + 0.070 0.000 -10.520
ENSG00000127928 E012 0.5149111 0.0216928631 1.110220e-01 2.070482e-01 7 93906592 93906593 2 + 0.070 0.346 2.810
ENSG00000127928 E013 0.5911836 0.0405405439 6.325248e-03 1.946966e-02 7 93906594 93906644 51 + 0.000 0.452 14.212
ENSG00000127928 E014 0.9211812 0.0136968632 2.604715e-04 1.218679e-03 7 93906645 93906735 91 + 0.000 0.607 14.949
ENSG00000127928 E015 6.3541356 0.0408437859 5.042717e-05 2.839772e-04 7 93906736 93906842 107 + 0.577 1.174 2.327
ENSG00000127928 E016 1.5188264 0.0097559259 8.107513e-01 8.799450e-01 7 93909483 93910789 1307 + 0.379 0.346 -0.191
ENSG00000127928 E017 5.9044394 0.0033318523 7.035764e-04 2.911861e-03 7 93910790 93911265 476 + 0.634 1.078 1.730